Summary of Study ST001220
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000819. The data can be accessed directly via it's Project DOI: 10.21228/M8TD5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001220 |
| Study Title | Effects of cadmium exposure on the bumble bee metabolome |
| Study Summary | We exposed worker bumble bees to cadmium chloride or control through diet. We then used LCMS untargeted metabolomics on pools of 3 bees each. |
| Institute | University of California, Riverside |
| Last Name | Rothman |
| First Name | Jason |
| Address | 900 University Ave., Riverside, CA, 91766, USA |
| jroth002@ucr.edu | |
| Phone | 9518275817 |
| Submit Date | 2019-07-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000819 |
| Project DOI: | doi: 10.21228/M8TD5C |
| Project Title: | Untargeted metabolomics of bumble bees exposed to cadmium |
| Project Summary: | Bumble bee workers were exposed to cadmium chloride or control through diet. We then ran untargeted metabolomics on the bees. |
| Institute: | University of California, Riverside |
| Last Name: | Rothman |
| First Name: | Jason |
| Address: | 900 University Ave., Riverside, CA, 91766, USA |
| Email: | jroth002@ucr.edu |
| Phone: | 9518275817 |
Subject:
| Subject ID: | SU001287 |
| Subject Type: | Invertebrate |
| Subject Species: | Bombus impatiens |
| Taxonomy ID: | 132113 |
| Species Group: | Insects |
Factors:
Subject type: Invertebrate; Subject species: Bombus impatiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA086250 | BBCD-4 | Cadmium |
| SA086251 | BBCD-3 | Cadmium |
| SA086252 | BBCD-5 | Cadmium |
| SA086253 | BBCD-2 | Cadmium |
| SA086254 | BBCD-1 | Cadmium |
| SA086255 | BBCT-4 | Control |
| SA086256 | BBCT-3 | Control |
| SA086257 | BBCT-2 | Control |
| SA086258 | BBCT-1 | Control |
| SA086259 | BBCT-5 | Control |
| SA086260 | QC-1 | QCPool |
| SA086261 | QC-4 | QCPool |
| SA086262 | QC-3 | QCPool |
| SA086263 | QC-2 | QCPool |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO001281 |
| Collection Summary: | We immersed live bees in liquid nitrogen and stored the samples at -80 °C. |
| Sample Type: | Insect tissue |
Treatment:
| Treatment ID: | TR001302 |
| Treatment Summary: | We obtained five commercial Bombus impatiens colonies that each contained approximately 50 workers, a gravid queen, pollen and a Biogluc® feeder from the Biobest Group (Biobest USA Inc., Romulus, MI). We immediately replaced the Biogluc with sterile 60% sucrose and allowed the bees access ad libitum. We also provided the colony with pollen patties ad libitum and kept the colonies at 29 °C under constant darkness at the University of California, Riverside. We allowed the colonies to grow undisturbed for two weeks before starting the experiment. Once the colonies had populated, we transferred 10 bees (two cohorts of five) to 475 mL polypropylene containers (WebstaurantStore, Lancaster, PA) and reared them at 29 °C under 24-hour darkness. We then exposed the bees to treatments consisting of 60% sucrose spiked with either 0.46 mg/kg cadmium chloride and used unspiked 60% sucrose as a control. |
Sample Preparation:
| Sampleprep ID: | SP001295 |
| Sampleprep Summary: | We then pooled three bee abdomens from each cage, freeze-dried the samples, and homogenized the abdomens to a fine powder at 4° C using a bead mill homogenizer. Next, we extracted 10-12 mg of the powder in a 1.5 mL tube with 100 µL of ice-cold extraction solvent (30:30:20:20 acetonitrile:methanol:water:isopropanol) per 1 mg of tissue. We sonicated the samples for 5 minutes in an ice bath, then vortexed them for 30 min at 4° C. Lastly, we centrifuged the samples at 16,000 x g for 15 min at 4° C. |
Combined analysis:
| Analysis ID | AN002033 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt-G2-Si |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001473 |
| Chromatography Summary: | We used a Synapt G2-Si quadrupole time-of-flight mass spectrometer (Waters, Milford, MA) coupled to an I-class UPLC system (Waters) for LC-MS analyses in the UC Riverside Metabolomics Core Facility. We carried out separations on a CSH phenyl-hexyl column (2.1 x 100 mm, 1.7 µM) (Waters, Milford, MA), with the following mobile phases: A. Water with 0.1% formic acid and B. Acetonitrile with 0.1% formic acid at a flow rate of 250 µL/min at 40° C. We injected 2 µL of sample extract, and the gradient was as follows: 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
| Methods Filename: | jroth002_LC_method.docx |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity CSH Phenyl-Hexyl (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001885 |
| Analysis ID: | AN002033 |
| Instrument Name: | Waters Synapt-G2-Si |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | We operated the MS in positive ion mode (50 to 1200 m/z) with a 100 ms scan time and acquired MS/MS data at 1 MS/MS scan per MS scan. We set source and desolvation temperatures to 150° C and 600° C, respectively. We set the desolvation gas flow to 1100 L/hr and cone gas flow to 150 L/h, with all gases being nitrogen except the collision gas, which was argon, and set capillary voltage to 1 kV. We generated a quality control sample by pooling equal aliquots of each sample and analyzed this pool every 3-4 injections to monitor system stability and performance. We analyzed samples in random order and used a leucine enkephalin infusion for mass correction. We processed the metabolite data (peak picking, alignment, deconvolution, integration, normalization, and spectral matching) with Progenesis Qi software (Nonlinear Dynamics, Durham, NC). We normalized the resulting data to total ion abundance and removed features with a coefficient of variation greater than 20% or an average abundance less than 200 in the quality control injections as in Barupal et al. 2018 and Dunn et al. 2011 (Barupal et al., 2018; Dunn et al., 2011). To aid in the identification of features belonging to the same metabolite, we assigned features a cluster ID using RAMClust (Broeckling, Afsar, Neumann, Ben-Hur, & Prenni, 2014). Next, we used a slightly modified version of the metabolomics standard initiative guidelines to assign annotation level confidence (Schymanski et al., 2014; Sumner et al., 2007): Annotation level 1 indicates an MS and MS/MS match or MS and retention time match to an in-house database generated with authentic standards. Level 2a indicates an MS and MS/MS match to an external database. Level 2b indicates an MS and MS/MS match to the LipidBlast database (Kind et al., 2013) or an MS match and diagnostic evidence (i.e. the dominant presence of an m/z 85 fragment ion for acylcarnitines). We searched against several mass spectral metabolite databases including Metlin, Massbank of North America (Blaženović et al., 2019; Kind et al., 2013), and an in-house database in the UC Riverside Metabolomics Core Facility. |
| Ion Mode: | POSITIVE |
