Summary of Study ST001227

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000822. The data can be accessed directly via it's Project DOI: 10.21228/M8F69D This work is supported by NIH grant, U2C- DK119886.

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Study IDST001227
Study TitleAquamin and Prevention of Colon Cancer (part-III)
Study TypeMS analysis
Study SummaryWe propose to evaluate microbial and metabolic profiles in baseline and endpoint colonic mucosal, fecal, and serum samples from human patients at risk for CRC and enrolled in a 90-day phase I clinical trial. Patients will receive daily supplementation with calcium alone, a calcium-rich multimineral (Aquamin?), or placebo (maltodextrin) (n=10 per group). We hypothesize that dietary supplementation will correlate with CRC-protective metabolic profiles and that multimineral supplementation will generate more favorable profiles than calcium supplementation alone.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-07-24
Num Groups24
Total Subjects18
Study CommentsColorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death when both genders are combined. Epidemiologically, calcium intake has been protective against colonic adenomas and even colon cancer. Calcium supplementation has reduced the risk of colon adenoma formation in subjects with a history of previous colon polyps. The utility of calcium supplementation for colon cancer prevention is somewhat modulated by the modest or inconsistent level of protection afforded. Our preliminary data in mice and human enterocyte models shows that dietary supplementation with a multimineral supplement (Aquamin?) containing calcium in combination with 72 measureable trace minerals is more protective against tumors and epithelial growth dysregulation than calcium alone. One potential mechanism, supported by our rodent data, is that multimineral supplementation alters gut microbial populations to generate bile acid and short chain fatty acid (SCFA) profiles that are CRC-protective.
Raw Data AvailableYes
Analysis Type DetailGC-MS
Release Date2019-09-23
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8F69D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000822
Project DOI:doi: 10.21228/M8F69D
Project Title:Aquamin and Prevention of Colon Cancer
Project Type:MS analysis
Project Summary:Effects of calcium and multi-mineral supplementation on gut microbes and microbially-derived metabolites in patients at risk for colon cancer
Institute:University of Michigan
Department:Pathology
Laboratory:Aslam Lab
Last Name:Aslam
First Name:Muhammad Nadeem
Address:Ann Arbor, MI
Email:mnaslam@umich.edu
Phone:734-936-1897

Subject:

Subject ID:SU001294
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time Point
SA086933S00031741Baseline
SA086934S00031739Baseline
SA086935S00031738Baseline
SA086936S00031742Baseline
SA086937S00031743Baseline
SA086938S00031727Baseline
SA086939S00031744Baseline
SA086940S00031737Baseline
SA086941S00031740Baseline
SA086942S00031731Baseline
SA086943S00031730Baseline
SA086944S00031736Baseline
SA086945S00031728Baseline
SA086946S00031732Baseline
SA086947S00031729Baseline
SA086948S00031733Baseline
SA086949S00031734Baseline
SA086950S00031735Baseline
SA086951S00031758Day 90
SA086952S00031756Day 90
SA086953S00031757Day 90
SA086954S00031759Day 90
SA086955S00031761Day 90
SA086956S00031755Day 90
SA086957S00031762Day 90
SA086958S00031760Day 90
SA086959S00031745Day 90
SA086960S00031748Day 90
SA086961S00031747Day 90
SA086962S00031746Day 90
SA086963S00031749Day 90
SA086964S00031750Day 90
SA086965S00031753Day 90
SA086966S00031752Day 90
SA086967S00031751Day 90
SA086968S00031754Day 90
Showing results 1 to 36 of 36

Collection:

Collection ID:CO001288
Collection Summary:Not available
Sample Type:Colon

Treatment:

Treatment ID:TR001309
Treatment Summary:Not available.

Sample Preparation:

Sampleprep ID:SP001302
Sampleprep Summary:Cold extraction of short chain fatty acids, measured by EI- GCMS without derivatization. SCFA species are reported as uM, with CV's generally ~10%.
Sampleprep Protocol Filename:Short_chain_fatty_acids_analysis.doc

Combined analysis:

Analysis ID AN002040
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Agilent HP5-MS (15m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units nmol/mg (wet weight)

Chromatography:

Chromatography ID:CH001480
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS001892
Analysis ID:AN002040
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:NA
Ion Mode:POSITIVE
Acquisition Parameters File:WAXPLUS_100-250C_3_SPLITLESS_SIM.M.zip
Analysis Protocol File:Short_chain_fatty_acids_analysis.doc
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