Summary of Study ST001228

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000822. The data can be accessed directly via it's Project DOI: 10.21228/M8F69D This work is supported by NIH grant, U2C- DK119886.

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Study IDST001228
Study TitleAquamin and Prevention of Colon Cancer (part-IV)
Study TypeMS analysis
Study SummaryWe propose to evaluate microbial and metabolic profiles in baseline and endpoint colonic mucosal, fecal, and serum samples from human patients at risk for CRC and enrolled in a 90-day phase I clinical trial. Patients will receive daily supplementation with calcium alone, a calcium-rich multimineral (Aquamin?), or placebo (maltodextrin) (n=10 per group). We hypothesize that dietary supplementation will correlate with CRC-protective metabolic profiles and that multimineral supplementation will generate more favorable profiles than calcium supplementation alone.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-07-24
Num Groups24
Total Subjects18
Study CommentsColorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death when both genders are combined. Epidemiologically, calcium intake has been protective against colonic adenomas and even colon cancer. Calcium supplementation has reduced the risk of colon adenoma formation in subjects with a history of previous colon polyps. The utility of calcium supplementation for colon cancer prevention is somewhat modulated by the modest or inconsistent level of protection afforded. Our preliminary data in mice and human enterocyte models shows that dietary supplementation with a multimineral supplement (Aquamin?) containing calcium in combination with 72 measureable trace minerals is more protective against tumors and epithelial growth dysregulation than calcium alone. One potential mechanism, supported by our rodent data, is that multimineral supplementation alters gut microbial populations to generate bile acid and short chain fatty acid (SCFA) profiles that are CRC-protective.
Raw Data AvailableYes
Analysis Type DetailGC-MS
Release Date2019-09-23
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8F69D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000822
Project DOI:doi: 10.21228/M8F69D
Project Title:Aquamin and Prevention of Colon Cancer
Project Type:MS analysis
Project Summary:Effects of calcium and multi-mineral supplementation on gut microbes and microbially-derived metabolites in patients at risk for colon cancer
Institute:University of Michigan
Department:Pathology
Laboratory:Aslam Lab
Last Name:Aslam
First Name:Muhammad Nadeem
Address:Ann Arbor, MI
Email:mnaslam@umich.edu
Phone:734-936-1897

Subject:

Subject ID:SU001295
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time Point
SA086969S00031777Baseline
SA086970S00031775Baseline
SA086971S00031774Baseline
SA086972S00031778Baseline
SA086973S00031779Baseline
SA086974S00031763Baseline
SA086975S00031780Baseline
SA086976S00031773Baseline
SA086977S00031776Baseline
SA086978S00031767Baseline
SA086979S00031766Baseline
SA086980S00031772Baseline
SA086981S00031764Baseline
SA086982S00031768Baseline
SA086983S00031765Baseline
SA086984S00031769Baseline
SA086985S00031770Baseline
SA086986S00031771Baseline
SA086987S00031794Day 90
SA086988S00031792Day 90
SA086989S00031793Day 90
SA086990S00031795Day 90
SA086991S00031797Day 90
SA086992S00031791Day 90
SA086993S00031798Day 90
SA086994S00031796Day 90
SA086995S00031781Day 90
SA086996S00031784Day 90
SA086997S00031783Day 90
SA086998S00031782Day 90
SA086999S00031785Day 90
SA087000S00031786Day 90
SA087001S00031789Day 90
SA087002S00031788Day 90
SA087003S00031787Day 90
SA087004S00031790Day 90
Showing results 1 to 36 of 36

Collection:

Collection ID:CO001289
Collection Summary:Not available
Sample Type:Feces

Treatment:

Treatment ID:TR001310
Treatment Summary:Not avaialble

Sample Preparation:

Sampleprep ID:SP001303
Sampleprep Summary:Cold extraction of short chain fatty acids, measured by EI- GCMS without derivatization. SCFA species are reported as uM, with CV's generally ~10%.
Sampleprep Protocol Filename:Short_chain_fatty_acids_analysis.doc

Combined analysis:

Analysis ID AN002041
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Agilent HP5-MS (15m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units nmol/mg (wet weight)

Chromatography:

Chromatography ID:CH001481
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS001893
Analysis ID:AN002041
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:Not available.
Ion Mode:POSITIVE
Acquisition Parameters File:WAXPLUS_100-200C_3_SPLIT10-1_SIM.M.zip
Analysis Protocol File:Short_chain_fatty_acids_analysis.doc
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