Summary of Study ST001257

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000843. The data can be accessed directly via it's Project DOI: 10.21228/M8QH5G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001257
Study TitleNutrimetabolomics and DASH diet
Study SummaryAlthough health benefits of the Dietary Approaches to Stop Hypertension (DASH) diet are established, it is not understood which food compounds result in these benefits. We used a step-wise approach to identify unique compounds from individual foods of a DASH-style diet, determined if these Food-Specific Compounds (FSC) are detectable in urine, and then examined relationships between urinary FSC and blood pressure (BP). Nineteen subjects were randomized into 6-week controlled DASH-style diet interventions. Untargeted, LC/MS-based metabolomics was performed on 24-hour urine samples collected before and after each intervention and on 12 representative DASH-style foods.
Institute
University of Colorado Denver
DepartmentAnschutz Medical Campus
Last NameReisdorph
First NameNichole
Address12850 East Montview Blvd, Aurora, CO, 80045, USA
Emailnichole.reisdorph@ucdenver.edu
Phone3037249234
Submit Date2019-09-22
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2020-09-22
Release Version1
Nichole Reisdorph Nichole Reisdorph
https://dx.doi.org/10.21228/M8QH5G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000843
Project DOI:doi: 10.21228/M8QH5G
Project Title:Foodomics Dash Diet
Project Summary:Untargeted metabolomics was used to analyze urine and a variety of foods from the Dietary Approaches to Stop Hypertension (DASH) diet in order to understand which food compounds result in health benefits.
Institute:University of Colorado Denver
Department:Anschutz Medical Campus
Last Name:Reisdorph
First Name:Nichole
Address:12850 East Montview Blvd, Aurora, CO, 80045, USA
Email:nichole.reisdorph@ucdenver.edu
Phone:3037249234
Funding Source:NIH (NIDDK, NCATS, NCRR), National Pork Board, NIH Indiana CCTSI, USDA

Subject:

Subject ID:SU001325
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample type Food type Pre or Post and food
SA09130802 Apple JuiceFood Apple Juice na
SA09130905 AppleFood Apple na
SA09131012 Beef TenderloinFood Beef na
SA09131103 BlueberriesFood Blueberries na
SA09131204 BroccoliFood Broccoli na
SA09131309 Chicken TenderloinFood Chicken na
SA09131413 Coffee dilutedFood Coffee diluted na
SA09131514 Coffee NeatFood Coffee neat na
SA09131611 coffeeFood Coffee na
SA09131707 CucumberFood Cucumber na
SA09131801 GrapefruitFood Grapefruit na
SA09131908 Peanut Butter 8 ulFood Peanut Butter na
SA09132006 Pork LoinFood Pork Loin na
SA09132110 TilapiaFood Tilapia na
SA091322S31-141 Post-ChUrine urine Post Chicken
SA091323S31-148 Post-ChUrine urine Post Chicken
SA091324S31-107 Post-ChUrine urine Post Chicken
SA091325S31-109 Post-ChUrine urine Post Chicken
SA091326S31-108 Post-ChUrine urine Post Chicken
SA091327S31-114 Post-ChUrine urine Post Chicken
SA091328S31-119 Post PoUrine urine Post Chicken
SA091329S31-116 Post-ChUrine urine Post Chicken
SA091330S31-115 Post-ChUrine urine Post Chicken
SA091331S31-120 Post-ChUrine urine Post Chicken
SA091332S31-111 Post-ChUrine urine Post Chicken
SA091333S31-106 Post-ChUrine urine Post Chicken
SA091334S31-144 Post ChUrine urine Post Chicken
SA091335S31-142 Post-ChUrine urine Post Chicken
SA091336S31-105 Post-ChUrine urine Post Chicken
SA091337S31-145 Post-ChUrine urine Post Chicken
SA091338S31-146 Post-ChUrine urine Post Chicken
SA091339S31-101 Post-ChUrine urine Post Chicken
SA091340S31-102 Post-ChUrine urine Post Chicken
SA091341S31-111 Post-PoUrine urine Post Pork
SA091342S31-114 Post-PoUrine urine Post Pork
SA091343S31-102 Post-PoUrine urine Post Pork
SA091344S31-146 Post-PoUrine urine Post Pork
SA091345S31-115 Post-PoUrine urine Post Pork
SA091346S31-101 Post-PoUrine urine Post Pork
SA091347S31-142 Post-PoUrine urine Post Pork
SA091348S31-116 Post-PoUrine urine Post Pork
SA091349S31-109 Post-PoUrine urine Post Pork
SA091350S31-108 Post PoUrine urine Post Pork
SA091351S31-141 Post-PoUrine urine Post Pork
SA091352S31-107 Post-PoUrine urine Post Pork
SA091353S31-148 Post-PoUrine urine Post Pork
SA091354S31-105 Post-PoUrine urine Post Pork
SA091355S31-145 Post-PoUrine urine Post Pork
SA091356S31-119 Post-ChUrine urine Post Pork
SA091357S31-106 Post-PoUrine urine Post Pork
SA091358S31-144 Post-PoUrine urine Post Pork
SA091359S31-120 Post-PoUrine urine Post Pork
SA091360S31-141 Pre-ChUrine urine Pre Chicken
SA091361S31-142 Pre-ChUrine urine Pre Chicken
SA091362S31-120 Pre-ChUrine urine Pre Chicken
SA091363S31-145 Pre-ChUrine urine Pre Chicken
SA091364S31-119 Pre-ChUrine urine Pre Chicken
SA091365S31-108 Pre-ChUrine urine Pre Chicken
SA091366S31-148 Pre-ChUrine urine Pre Chicken
SA091367S31-106 Pre-ChUrine urine Pre Chicken
SA091368S31-105 Pre-ChUrine urine Pre Chicken
SA091369S31-102 Pre-ChUrine urine Pre Chicken
SA091370S31-101 Pre-ChUrine urine Pre Chicken
SA091371S31-107 Pre-ChUrine urine Pre Chicken
SA091372S31-144 Pre-ChUrine urine Pre Chicken
SA091373S31-114 Pre-ChUrine urine Pre Chicken
SA091374S31-115 Pre-ChUrine urine Pre Chicken
SA091375S31-111 Pre-ChUrine urine Pre Chicken
SA091376S31-146 Pre ChUrine urine Pre Chicken
SA091377S31-116 Pre-ChUrine urine Pre Chicken
SA091378S31-109 Pre-ChUrine urine Pre Chicken
SA091379S31-144 Pre-PoUrine urine Pre Pork
SA091380S31-146 Pre-PoUrine urine Pre Pork
SA091381S31-148 Pre-PoUrine urine Pre Pork
SA091382S31-145 Pre-PoUrine urine Pre Pork
SA091383S31-111 Pre-PoUrine urine Pre Pork
SA091384S31-107 Pre-PoUrine urine Pre Pork
SA091385S31-108 Pre-PoUrine urine Pre Pork
SA091386S31-106 Pre-PoUrine urine Pre Pork
SA091387S31-105 Pre-PoUrine urine Pre Pork
SA091388S31-101 Pre-PoUrine urine Pre Pork
SA091389S31-102 Pre-PoUrine urine Pre Pork
SA091390S31-109 Pre-PoUrine urine Pre Pork
SA091391S31-114 Pre-PoUrine urine Pre Pork
SA091392S31-120 Pre-PoUrine urine Pre Pork
SA091393S31-141 Pre-PoUrine urine Pre Pork
SA091394S31-119 Pre-PoUrine urine Pre Pork
SA091395S31-116 Pre-PoUrine urine Pre Pork
SA091396S31-115 Pre-PoUrine urine Pre Pork
SA091397S31-142 Pre-PoUrine urine Pre Pork
Showing results 1 to 90 of 90

Collection:

Collection ID:CO001319
Collection Summary:Twenty-four-hour urine collections were obtained before and near the end of each of the two DASH-style diet periods. A total of 76 urine samples were used for untargeted metabolomics analysis, including 38 collected while the 19 participants consumed their typical diets (19 before each intervention period) and 38 while the DASH-style diet was consumed (19 during each intervention period). For simplicity, these are referred to as pre- and post-diet urine samples. Food was purchased from a local grocery store for metabolomic analyses. All four types of meat included in the diet (pork, chicken, fish, and beef loin) were analyzed. Fruits and vegetables, if not pre-washed, were washed with tap water and prepared with inedible parts (i.e. leaves or peels) removed. Meat and eggs were cooked as instructed to match prescribed cooked weight.
Sample Type:Urine
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001340
Treatment Summary:During the 18-week study period, participants initially consumed their self-chosen, unrestricted (typical) diets for two weeks. Participants were then randomly assigned to consume a DASH-style diet with either lean pork or chicken and fish as the predominant sources of dietary protein for six weeks. This time period was followed by a four-week washout period during which participants again consumed their typical diets prior to consuming the DASH-style diet containing the other predominant protein sources. Samples utilized for the current research consisted of 12 individual foods consumed during the DASH-style diet and urine samples collected prior to each intervention and during the final two weeks of each controlled feeding period.

Sample Preparation:

Sampleprep ID:SP001333
Sampleprep Summary:Individual food and urine samples were stored at -80 °C prior to sample preparation. Individual foods (including apple juice) were individually dried using a FreeZone 2.5 plus lyophilizer (Labconco, Kansas City, MO, USA). Dried samples were then divided and macerated using a chilled bio-pulverizer (BioSpec Products, Bartlesville, OK). Approximately 50 mg of each sample was measured into chilled microcentrifuge tubes and stored at -80o C prior to further processes and analysis. 10 µL of coffee was diluted with 90 µL of water, vortexed, aliquotted and stored at -80° C until analysis. An aliquot of coffee was also reserved for neat (i.e. unprocessed) analysis, and stored at -80° C until analysis. Freeze-dried foods were removed from -80° C and allowed to thaw on ice. To each sample, 480 μl of chilled methanol, 10 µl each of a hydrophilic spike mix, hydrophilic positive control mix, hydrophobic spike mix and hydrophobic positive control mix were added (see Supplemental Table S1). Samples were gently vortexed for 10 seconds, placed at -80o C for 60 minutes, and then centrifuged 0o C at 18,000 x g for 15 minutes to facilitate protein precipitation. Supernatants were transferred to new microcentrifuge tubes and dried using vacuum centrifugation at 45o C for approximately 60 minutes. Each sample was suspended in 50 µl of 95:5 LC/MS grade water-acetonitrile and gently vortexed for 30 seconds. Following a quick centrifugation, each sample was then divided into replicate 20 µl aliquots, while 10 µl was removed from each sample to generate a pooled QC sample. Samples were stored at -80° C until analysis. Urine samples were analyzed neat (i.e. without any preparation) except that authentic standards were spiked into urine samples to monitor variability in instrument response or batch effects.
Processing Storage Conditions:Described in summary

Combined analysis:

Analysis ID AN002086
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 6520
Column Agilent Zorbax Rapid Resolution HT (RRHT) SB-AQ (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode POSITIVE
Units Abundance (Counts Log2 Transformed)

Chromatography:

Chromatography ID:CH001523
Instrument Name:Agilent 6520
Column Name:Agilent Zorbax Rapid Resolution HT (RRHT) SB-AQ (100 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001937
Analysis ID:AN002086
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Agilent 6520 Time-of-Flight (TOF-MS) with dual electrospray ionization (ESI) source, scan rate 2.21 spectra/second, mass range 50-1700 m/z, gas temperature 325 °C, gas flow 12.0 L/min, nebulizer 30 psi, skimmer 60 V, capillary voltage 4000 V, fragmentor 120 V, reference masses 121.050873 and 922.009798 (Reference mix, Agilent Technologies,).
Ion Mode:POSITIVE
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