Summary of Study ST001257

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000843. The data can be accessed directly via it's Project DOI: 10.21228/M8QH5G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001257
Study Title	Nutrimetabolomics and DASH diet
Study Summary	Although health benefits of the Dietary Approaches to Stop Hypertension (DASH) diet are established, it is not understood which food compounds result in these benefits. We used a step-wise approach to identify unique compounds from individual foods of a DASH-style diet, determined if these Food-Specific Compounds (FSC) are detectable in urine, and then examined relationships between urinary FSC and blood pressure (BP). Nineteen subjects were randomized into 6-week controlled DASH-style diet interventions. Untargeted, LC/MS-based metabolomics was performed on 24-hour urine samples collected before and after each intervention and on 12 representative DASH-style foods.
Institute	University of Colorado Denver
Department	Anschutz Medical Campus
Last Name	Reisdorph
First Name	Nichole
Address	12850 East Montview Blvd, Aurora, CO, 80045, USA
Email	nichole.reisdorph@ucdenver.edu
Phone	3037249234
Submit Date	2019-09-22
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2020-09-22
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000843
Project DOI:	doi: 10.21228/M8QH5G
Project Title:	Foodomics Dash Diet
Project Summary:	Untargeted metabolomics was used to analyze urine and a variety of foods from the Dietary Approaches to Stop Hypertension (DASH) diet in order to understand which food compounds result in health benefits.
Institute:	University of Colorado Denver
Department:	Anschutz Medical Campus
Last Name:	Reisdorph
First Name:	Nichole
Address:	12850 East Montview Blvd, Aurora, CO, 80045, USA
Email:	nichole.reisdorph@ucdenver.edu
Phone:	3037249234
Funding Source:	NIH (NIDDK, NCATS, NCRR), National Pork Board, NIH Indiana CCTSI, USDA

Subject:

Subject ID:	SU001325
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample type	Food type	Pre or Post and food
SA091308	02 Apple Juice	Food	Apple Juice	na
SA091309	05 Apple	Food	Apple	na
SA091310	12 Beef Tenderloin	Food	Beef	na
SA091311	03 Blueberries	Food	Blueberries	na
SA091312	04 Broccoli	Food	Broccoli	na
SA091313	09 Chicken Tenderloin	Food	Chicken	na
SA091314	13 Coffee diluted	Food	Coffee diluted	na
SA091315	14 Coffee Neat	Food	Coffee neat	na
SA091316	11 coffee	Food	Coffee	na
SA091317	07 Cucumber	Food	Cucumber	na
SA091318	01 Grapefruit	Food	Grapefruit	na
SA091319	08 Peanut Butter 8 ul	Food	Peanut Butter	na
SA091320	06 Pork Loin	Food	Pork Loin	na
SA091321	10 Tilapia	Food	Tilapia	na
SA091322	S31-141 Post-Ch	Urine	urine	Post Chicken
SA091323	S31-148 Post-Ch	Urine	urine	Post Chicken
SA091324	S31-107 Post-Ch	Urine	urine	Post Chicken
SA091325	S31-109 Post-Ch	Urine	urine	Post Chicken
SA091326	S31-108 Post-Ch	Urine	urine	Post Chicken
SA091327	S31-114 Post-Ch	Urine	urine	Post Chicken
SA091328	S31-119 Post Po	Urine	urine	Post Chicken
SA091329	S31-116 Post-Ch	Urine	urine	Post Chicken
SA091330	S31-115 Post-Ch	Urine	urine	Post Chicken
SA091331	S31-120 Post-Ch	Urine	urine	Post Chicken
SA091332	S31-111 Post-Ch	Urine	urine	Post Chicken
SA091333	S31-106 Post-Ch	Urine	urine	Post Chicken
SA091334	S31-144 Post Ch	Urine	urine	Post Chicken
SA091335	S31-142 Post-Ch	Urine	urine	Post Chicken
SA091336	S31-105 Post-Ch	Urine	urine	Post Chicken
SA091337	S31-145 Post-Ch	Urine	urine	Post Chicken
SA091338	S31-146 Post-Ch	Urine	urine	Post Chicken
SA091339	S31-101 Post-Ch	Urine	urine	Post Chicken
SA091340	S31-102 Post-Ch	Urine	urine	Post Chicken
SA091341	S31-111 Post-Po	Urine	urine	Post Pork
SA091342	S31-114 Post-Po	Urine	urine	Post Pork
SA091343	S31-102 Post-Po	Urine	urine	Post Pork
SA091344	S31-146 Post-Po	Urine	urine	Post Pork
SA091345	S31-115 Post-Po	Urine	urine	Post Pork
SA091346	S31-101 Post-Po	Urine	urine	Post Pork
SA091347	S31-142 Post-Po	Urine	urine	Post Pork
SA091348	S31-116 Post-Po	Urine	urine	Post Pork
SA091349	S31-109 Post-Po	Urine	urine	Post Pork
SA091350	S31-108 Post Po	Urine	urine	Post Pork
SA091351	S31-141 Post-Po	Urine	urine	Post Pork
SA091352	S31-107 Post-Po	Urine	urine	Post Pork
SA091353	S31-148 Post-Po	Urine	urine	Post Pork
SA091354	S31-105 Post-Po	Urine	urine	Post Pork
SA091355	S31-145 Post-Po	Urine	urine	Post Pork
SA091356	S31-119 Post-Ch	Urine	urine	Post Pork
SA091357	S31-106 Post-Po	Urine	urine	Post Pork
SA091358	S31-144 Post-Po	Urine	urine	Post Pork
SA091359	S31-120 Post-Po	Urine	urine	Post Pork
SA091360	S31-141 Pre-Ch	Urine	urine	Pre Chicken
SA091361	S31-142 Pre-Ch	Urine	urine	Pre Chicken
SA091362	S31-120 Pre-Ch	Urine	urine	Pre Chicken
SA091363	S31-145 Pre-Ch	Urine	urine	Pre Chicken
SA091364	S31-119 Pre-Ch	Urine	urine	Pre Chicken
SA091365	S31-108 Pre-Ch	Urine	urine	Pre Chicken
SA091366	S31-148 Pre-Ch	Urine	urine	Pre Chicken
SA091367	S31-106 Pre-Ch	Urine	urine	Pre Chicken
SA091368	S31-105 Pre-Ch	Urine	urine	Pre Chicken
SA091369	S31-102 Pre-Ch	Urine	urine	Pre Chicken
SA091370	S31-101 Pre-Ch	Urine	urine	Pre Chicken
SA091371	S31-107 Pre-Ch	Urine	urine	Pre Chicken
SA091372	S31-144 Pre-Ch	Urine	urine	Pre Chicken
SA091373	S31-114 Pre-Ch	Urine	urine	Pre Chicken
SA091374	S31-115 Pre-Ch	Urine	urine	Pre Chicken
SA091375	S31-111 Pre-Ch	Urine	urine	Pre Chicken
SA091376	S31-146 Pre Ch	Urine	urine	Pre Chicken
SA091377	S31-116 Pre-Ch	Urine	urine	Pre Chicken
SA091378	S31-109 Pre-Ch	Urine	urine	Pre Chicken
SA091379	S31-144 Pre-Po	Urine	urine	Pre Pork
SA091380	S31-146 Pre-Po	Urine	urine	Pre Pork
SA091381	S31-148 Pre-Po	Urine	urine	Pre Pork
SA091382	S31-145 Pre-Po	Urine	urine	Pre Pork
SA091383	S31-111 Pre-Po	Urine	urine	Pre Pork
SA091384	S31-107 Pre-Po	Urine	urine	Pre Pork
SA091385	S31-108 Pre-Po	Urine	urine	Pre Pork
SA091386	S31-106 Pre-Po	Urine	urine	Pre Pork
SA091387	S31-105 Pre-Po	Urine	urine	Pre Pork
SA091388	S31-101 Pre-Po	Urine	urine	Pre Pork
SA091389	S31-102 Pre-Po	Urine	urine	Pre Pork
SA091390	S31-109 Pre-Po	Urine	urine	Pre Pork
SA091391	S31-114 Pre-Po	Urine	urine	Pre Pork
SA091392	S31-120 Pre-Po	Urine	urine	Pre Pork
SA091393	S31-141 Pre-Po	Urine	urine	Pre Pork
SA091394	S31-119 Pre-Po	Urine	urine	Pre Pork
SA091395	S31-116 Pre-Po	Urine	urine	Pre Pork
SA091396	S31-115 Pre-Po	Urine	urine	Pre Pork
SA091397	S31-142 Pre-Po	Urine	urine	Pre Pork

Showing results 1 to 90 of 90

Showing results 1 to 90 of 90

Collection:

Collection ID:	CO001319
Collection Summary:	Twenty-four-hour urine collections were obtained before and near the end of each of the two DASH-style diet periods. A total of 76 urine samples were used for untargeted metabolomics analysis, including 38 collected while the 19 participants consumed their typical diets (19 before each intervention period) and 38 while the DASH-style diet was consumed (19 during each intervention period). For simplicity, these are referred to as pre- and post-diet urine samples. Food was purchased from a local grocery store for metabolomic analyses. All four types of meat included in the diet (pork, chicken, fish, and beef loin) were analyzed. Fruits and vegetables, if not pre-washed, were washed with tap water and prepared with inedible parts (i.e. leaves or peels) removed. Meat and eggs were cooked as instructed to match prescribed cooked weight.
Sample Type:	Urine
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR001340
Treatment Summary:	During the 18-week study period, participants initially consumed their self-chosen, unrestricted (typical) diets for two weeks. Participants were then randomly assigned to consume a DASH-style diet with either lean pork or chicken and fish as the predominant sources of dietary protein for six weeks. This time period was followed by a four-week washout period during which participants again consumed their typical diets prior to consuming the DASH-style diet containing the other predominant protein sources. Samples utilized for the current research consisted of 12 individual foods consumed during the DASH-style diet and urine samples collected prior to each intervention and during the final two weeks of each controlled feeding period.

Treatment ID:

TR001340

Treatment Summary:

During the 18-week study period, participants initially consumed their self-chosen, unrestricted (typical) diets for two weeks. Participants were then randomly assigned to consume a DASH-style diet with either lean pork or chicken and fish as the predominant sources of dietary protein for six weeks. This time period was followed by a four-week washout period during which participants again consumed their typical diets prior to consuming the DASH-style diet containing the other predominant protein sources. Samples utilized for the current research consisted of 12 individual foods consumed during the DASH-style diet and urine samples collected prior to each intervention and during the final two weeks of each controlled feeding period.

Sample Preparation:

Sampleprep ID:	SP001333
Sampleprep Summary:	Individual food and urine samples were stored at -80 °C prior to sample preparation. Individual foods (including apple juice) were individually dried using a FreeZone 2.5 plus lyophilizer (Labconco, Kansas City, MO, USA). Dried samples were then divided and macerated using a chilled bio-pulverizer (BioSpec Products, Bartlesville, OK). Approximately 50 mg of each sample was measured into chilled microcentrifuge tubes and stored at -80o C prior to further processes and analysis. 10 µL of coffee was diluted with 90 µL of water, vortexed, aliquotted and stored at -80° C until analysis. An aliquot of coffee was also reserved for neat (i.e. unprocessed) analysis, and stored at -80° C until analysis. Freeze-dried foods were removed from -80° C and allowed to thaw on ice. To each sample, 480 μl of chilled methanol, 10 µl each of a hydrophilic spike mix, hydrophilic positive control mix, hydrophobic spike mix and hydrophobic positive control mix were added (see Supplemental Table S1). Samples were gently vortexed for 10 seconds, placed at -80o C for 60 minutes, and then centrifuged 0o C at 18,000 x g for 15 minutes to facilitate protein precipitation. Supernatants were transferred to new microcentrifuge tubes and dried using vacuum centrifugation at 45o C for approximately 60 minutes. Each sample was suspended in 50 µl of 95:5 LC/MS grade water-acetonitrile and gently vortexed for 30 seconds. Following a quick centrifugation, each sample was then divided into replicate 20 µl aliquots, while 10 µl was removed from each sample to generate a pooled QC sample. Samples were stored at -80° C until analysis. Urine samples were analyzed neat (i.e. without any preparation) except that authentic standards were spiked into urine samples to monitor variability in instrument response or batch effects.
Processing Storage Conditions:	Described in summary

Combined analysis:

Analysis ID	AN002086
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Agilent 6520
Column	Agilent Zorbax Rapid Resolution HT (RRHT) SB-AQ (100 x 2.1mm,1.8um)
MS Type	ESI
MS instrument type	QTOF
MS instrument name	Agilent 6520 QTOF
Ion Mode	POSITIVE
Units	Abundance (Counts Log2 Transformed)

Chromatography:

Chromatography ID:	CH001523
Instrument Name:	Agilent 6520
Column Name:	Agilent Zorbax Rapid Resolution HT (RRHT) SB-AQ (100 x 2.1mm,1.8um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001937
Analysis ID:	AN002086
Instrument Name:	Agilent 6520 QTOF
Instrument Type:	QTOF
MS Type:	ESI
MS Comments:	Agilent 6520 Time-of-Flight (TOF-MS) with dual electrospray ionization (ESI) source, scan rate 2.21 spectra/second, mass range 50-1700 m/z, gas temperature 325 °C, gas flow 12.0 L/min, nebulizer 30 psi, skimmer 60 V, capillary voltage 4000 V, fragmentor 120 V, reference masses 121.050873 and 922.009798 (Reference mix, Agilent Technologies,).
Ion Mode:	POSITIVE