Summary of Study ST001277

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000862. The data can be accessed directly via it's Project DOI: 10.21228/M8896R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001277
Study TitlePseudoexfoliation Aqueous Humor Metabolites from Veterans Affairs Patients
Study SummaryWe performed high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) Isotopic Ratio Outlier Analysis of aqueous humor samples from patients from patients with pseudoefoliation syndrome (PEX).
Institute
University of Miami
DepartmentOphthalmology, Bascom Palmer Eye Institute
LaboratorySanjoy K. Bhattacharya
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Avenue, Miami, FL 33136
EmailSBhattacharya@med.miami.edu
Phone305-482-4103
Submit Date2019-11-14
Num Groups1
Total Subjects31
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-05-14
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M8896R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000862
Project DOI:doi: 10.21228/M8896R
Project Title:Pseudoexfoliation Syndrome Humor Metabolites from Veterans Affairs Patients
Project Type:untargeted LC-MS/MS IROA metabolomics
Project Summary:We performed high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) Isotopic Ratio Outlier Analysis of aqueous humor samples from patients with pseudoexfoliation syndrome (PEX).
Institute:University of Miami
Department:Ophthalmology, Bascom Palmer Eye Institute
Last Name:Bhattacharya
First Name:Sanjoy
Address:1638 NW 10th Avenue, Miami, Florida, 33136, USA
Email:sbhattacharya@med.miami.edu
Phone:3054824103
Contributors:Leila Abdelrahman

Subject:

Subject ID:SU001349
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment1
SA093235S0012_POSPXE
SA093236S0011_POSPXE
SA093237S0013_POSPXE
SA093238S0015_POSPXE
SA093239S0016_POSPXE
SA093240S0010_POSPXE
SA093241S0014_POSPXE
SA093242S008_POSPXE
SA093243S004_POSPXE
SA093244S003_POSPXE
SA093245S005_POSPXE
SA093246S006_POSPXE
SA093247S0017_POSPXE
SA093248S007_POSPXE
SA093249S009_POSPXE
SA093250S0018_POSPXE
SA093251S0027_POSPXE
SA093252S0026_POSPXE
SA093253S0028_POSPXE
SA093254S0029_POSPXE
SA093255S0031_POSPXE
SA093256S0030_POSPXE
SA093257S0025_POSPXE
SA093258S0024_POSPXE
SA093259S0020_POSPXE
SA093260S0019_POSPXE
SA093261S0021_POSPXE
SA093262S0022_POSPXE
SA093263S0023_POSPXE
SA093264S002_POSPXE
SA093265S001_POSPXE
SA093266S0011_NEGPXE
SA093267S0010_NEGPXE
SA093268S0012_NEGPXE
SA093269S0013_NEGPXE
SA093270S0015_NEGPXE
SA093271S0014_NEGPXE
SA093272S009_NEGPXE
SA093273S008_NEGPXE
SA093274S003_NEGPXE
SA093275S002_NEGPXE
SA093276S004_NEGPXE
SA093277S005_NEGPXE
SA093278S007_NEGPXE
SA093279S006_NEGPXE
SA093280S0016_NEGPXE
SA093281S0017_NEGPXE
SA093282S0027_NEGPXE
SA093283S0026_NEGPXE
SA093284S0028_NEGPXE
SA093285S0029_NEGPXE
SA093286S0031_NEGPXE
SA093287S0030_NEGPXE
SA093288S0025_NEGPXE
SA093289S0024_NEGPXE
SA093290S0019_NEGPXE
SA093291S0018_NEGPXE
SA093292S0020_NEGPXE
SA093293S0021_NEGPXE
SA093294S0023_NEGPXE
SA093295S0022_NEGPXE
SA093296S001_NEGPXE
Showing results 1 to 62 of 62

Collection:

Collection ID:CO001343
Collection Summary:All materials were collected from human donors without identifiers under institutional review board exemption/approval. We acquired 31 aqueous humor (AH) samples from participants at the Veterans Administration (VA) Medical Center (Miami, FL). Patient samples used for Isotopic Ratio Outlier Analysis (IROA) consisted of individuals with PEX (n=31).
Sample Type:Eye tissue
Collection Location:1201 NW 16th St, Miami, FL 33125

Treatment:

Treatment ID:TR001364
Treatment Summary:No Treatment

Sample Preparation:

Sampleprep ID:SP001357
Sampleprep Summary:The 31 samples were prepared for isotopic ratio outlier analysis (IROA). Approximately 800 µL of precipitate solution (8:1:1 Acetonitrile: Methanol: Acetone) was added to the 50 µL of aqueous humor. The metabolites were vortexed and incubated at 4 °C for 30 minutes. This was followed by another incubation period at -20 °C for 1 hour. Each sample was centrifuged at 20,000 x g for 10 minutes at 4 °C (Beckman Microfuge 18) to form a pellet and 375 µL of supernatant was collected. The supernatant was dried in a speed vacuum for approximately 20 minutes or until the sample was fully dry and stored at -20 °C. The IROA internal standard (IROA-IS, IROA technologies) (U-95% 13C) was reconstituted in 1.2 mL of LC-MS grade water. The samples were reconstituted in 25 µL of LC-MS grade water and 10 µL was combined with 20 µL of IROA-IS and subjected to LC-MS/MS analysis. A long-term reference standard (LTRS) was reconstituted in 50 µL of LC-MS grade water and subjected to LC-MS/MS as the first, last, and every 10 samples.

Combined analysis:

Analysis ID AN002118
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela 600
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units C12:C13 Ratio

Chromatography:

Chromatography ID:CH001551
Instrument Name:Thermo Accela 600
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Column Temperature:40 C
Flow Rate:350 ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS001973
Analysis ID:AN002118
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Samples were ran in positive and negative ion mode Data dependent MS/MS
Ion Mode:UNSPECIFIED
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