Summary of Study ST001292

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000874. The data can be accessed directly via it's Project DOI: 10.21228/M8Q975 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001292
Study TitleMetabolomics of blood plasma and kidney tissue from control (db/m) and diabetic (db/db) mice.
Study TypeMS/MSMS analysis
Study SummaryPlasma and kidney from control (db/m) and diabetic (db/db) mice on regular or eNOS -/- background. Some mice received treatment (20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age). Samples paired (plasma/tissue from individual mice). Samples snap frozen and stored at -80 until use.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address1000 wall St., Ann Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-12-16
Study CommentsThe wrong mouse number was recorded for P_29 and P_59 so they were excluded from analysis.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2019-12-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8Q975
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000874
Project DOI:doi: 10.21228/M8Q975
Project Title:Metabolomics of blood plasma and kidney tissue from control (db/m) and diabetic (db/db) mice.
Project Type:MS/MSMS analysis
Project Summary:Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models
Institute:Michigan Center for Translational Pathology
Department:UMich Internal Medicine
Laboratory:Pennathur Lab
Last Name:Pennathur
First Name:Subramaniam
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:spennath@umich.edu
Phone:734 615 5757

Subject:

Subject ID:SU001366
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id ACE/ARB Inhibitor control/diabetic eNOS background
SA093900S00030150No Treat db/db diabetic eNOS +/+
SA093901S00030144No Treat db/db diabetic eNOS +/+
SA093902S00030128No Treat db/db diabetic eNOS +/+
SA093903S00030157No Treat db/db diabetic eNOS +/+
SA093904S00030166No Treat db/db diabetic eNOS +/+
SA093905S00030173No Treat db/db diabetic eNOS +/+
SA093906S00030171No Treat db/db diabetic eNOS +/+
SA093907S00030121No Treat db/db diabetic eNOS +/+
SA093908S00030164No Treat db/db diabetic eNOS +/+
SA093909S00030136No Treat db/db diabetic eNOS +/+
SA093910S00030167No Treat db/db diabetic eNOS -/-
SA093911S00030117No Treat db/db diabetic eNOS -/-
SA093912S00030152No Treat db/db diabetic eNOS -/-
SA093913S00030131No Treat db/db diabetic eNOS -/-
SA093914S00030159No Treat db/db diabetic eNOS -/-
SA093915S00030098No Treat db/db diabetic eNOS -/-
SA093916S00030146No Treat db/db diabetic eNOS -/-
SA093917S00030139No Treat db/db diabetic eNOS -/-
SA093918S00030110No Treat db/db diabetic eNOS -/-
SA093919S00030170No Treat db/db diabetic eNOS -/-
SA093920S00030129No Treat db/m control eNOS +/+
SA093921S00030158No Treat db/m control eNOS +/+
SA093922S00030151No Treat db/m control eNOS +/+
SA093923S00030137No Treat db/m control eNOS +/+
SA093924S00030145No Treat db/m control eNOS +/+
SA093925S00030103No Treat db/m control eNOS +/+
SA093926S00030108No Treat db/m control eNOS +/+
SA093927S00030115No Treat db/m control eNOS +/+
SA093928S00030165No Treat db/m control eNOS +/+
SA093929S00030122No Treat db/m control eNOS +/+
SA093930S00030119No Treat db/m control eNOS -/-
SA093931S00030099No Treat db/m control eNOS -/-
SA093932S00030172No Treat db/m control eNOS -/-
SA093933S00030112No Treat db/m control eNOS -/-
SA093934S00030154No Treat db/m control eNOS -/-
SA093935S00030141No Treat db/m control eNOS -/-
SA093936S00030161No Treat db/m control eNOS -/-
SA093937S00030133No Treat db/m control eNOS -/-
SA093938S00030125No Treat db/m control eNOS -/-
SA093939S00030105No Treat db/m control eNOS -/-
SA093940S00030148Treat db/db diabetic eNOS +/+
SA093941S00030162Treat db/db diabetic eNOS +/+
SA093942S00030168Treat db/db diabetic eNOS +/+
SA093943S00030102Treat db/db diabetic eNOS +/+
SA093944S00030155Treat db/db diabetic eNOS +/+
SA093945S00030142Treat db/db diabetic eNOS +/+
SA093946S00030126Treat db/db diabetic eNOS +/+
SA093947S00030134Treat db/db diabetic eNOS +/+
SA093948S00030113Treat db/db diabetic eNOS +/+
SA093949S00030106Treat db/db diabetic eNOS +/+
SA093950S00030116Treat db/db diabetic eNOS -/-
SA093951S00030130Treat db/db diabetic eNOS -/-
SA093952S00030174Treat db/db diabetic eNOS -/-
SA093953S00030169Treat db/db diabetic eNOS -/-
SA093954S00030138Treat db/db diabetic eNOS -/-
SA093955S00030109Treat db/db diabetic eNOS -/-
SA093956S00030123Treat db/db diabetic eNOS -/-
SA093957S00030127Treat db/m control eNOS +/+
SA093958S00030114Treat db/m control eNOS +/+
SA093959S00030120Treat db/m control eNOS +/+
SA093960S00030163Treat db/m control eNOS +/+
SA093961S00030156Treat db/m control eNOS +/+
SA093962S00030149Treat db/m control eNOS +/+
SA093963S00030143Treat db/m control eNOS +/+
SA093964S00030107Treat db/m control eNOS +/+
SA093965S00030135Treat db/m control eNOS +/+
SA093966S00030101Treat db/m control eNOS +/+
SA093967S00030111Treat db/m control eNOS -/-
SA093968S00030100Treat db/m control eNOS -/-
SA093969S00030160Treat db/m control eNOS -/-
SA093970S00030140Treat db/m control eNOS -/-
SA093971S00030147Treat db/m control eNOS -/-
SA093972S00030153Treat db/m control eNOS -/-
SA093973S00030132Treat db/m control eNOS -/-
SA093974S00030124Treat db/m control eNOS -/-
SA093975S00030104Treat db/m control eNOS -/-
SA093976S00030118Treat db/m control eNOS -/-
Showing results 1 to 77 of 77

Collection:

Collection ID:CO001361
Collection Summary:Kidney samples snap-frozen and stored at -80 until use.
Sample Type:Kidney
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001381
Treatment Summary:Treatment group mice received 20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age), controls didn't receive any drugs.

Sample Preparation:

Sampleprep ID:SP001374
Sampleprep Summary:Standard tissue lipidomics protocol
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN002149 AN002150
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH001572
Instrument Name:Shimadzu Nexera X2
Column Name:Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH001573
Instrument Name:Shimadzu Nexera X2
Column Name:Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002000
Analysis ID:AN002149
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids
Ion Mode:POSITIVE
  
MS ID:MS002001
Analysis ID:AN002150
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids
Ion Mode:NEGATIVE
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