Summary of Study ST001295
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000876. The data can be accessed directly via it's Project DOI: 10.21228/M8FX1M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001295 |
| Study Title | Estimating Platelet Mitochondrial Function in Patients with Sepsis - WB NMRs (part-II) |
| Study Type | single timepoint |
| Study Summary | Relationships between platelet mitochondrial oxygen consumption rates (mOCR) and metabolites in platelets as measured by quantitative 1H-NMR metabolomics in WB. Comparison of mitochondrial function and metabolomics in patients with sepsis and non-sepsis ED patients |
| Institute | University of Michigan; University of Mississippi; University of Minnesota |
| Department | Clinical Pharmacy (UMich); Emergency Medicine (UMiss) |
| Laboratory | Stringer NMR Metabolomics Laboratory |
| Last Name | McHugh |
| First Name | Cora |
| Address | 428 Church St, Ann Arbor, MI, 48103, USA |
| NMRmetabolomics@umich.edu | |
| Phone | 7343530164 |
| Submit Date | 2019-12-13 |
| Num Groups | 2 |
| Total Subjects | 26 |
| Num Males | 14 |
| Num Females | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2020-05-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000876 |
| Project DOI: | doi: 10.21228/M8FX1M |
| Project Title: | Estimating Platelet Mitochondrial Function in Patients with Sepsis |
| Project Type: | Quantitative NMR Metabolomics |
| Project Summary: | Sepsis patients and ill controls admitted to the ED had matched whole blood (WB) and isolated platelet samples collected. The control group was chosen to represent an acutely ill 127cohort of the same racial, sex, and age as the sepsis cohort. NMR was run on extracted WB and platelets to determine how the metabolome reflects mitochondrial function |
| Institute: | University of Michigan; University of Mississippi; University of Minnesota |
| Department: | Clinical Pharmacy (UMich); Emergency Medicine (UMiss) |
| Laboratory: | Stringer NMR Metabolomics Laboratory |
| Last Name: | McHugh |
| First Name: | Cora |
| Address: | 428 Church St, Ann Arbor, MI, 48103, USA |
| Email: | NMRmetabolomics@umich.edu |
| Phone: | 7343530164 |
| Funding Source: | GM111400, K23GM113041 |
| Contributors: | Kathleen Stringer, Mike Puskarich, Marc McCann |
Subject:
| Subject ID: | SU001369 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 32-67 |
| Gender: | Male and female |
| Human Race: | Caucasian (C) and African American (AA) |
| Human Ethnicity: | non-hispanic |
| Human Inclusion Criteria: | Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc) |
| Human Exclusion Criteria: | Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent. |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Condition |
|---|---|---|
| SA094121 | 233_69 | Control |
| SA094122 | 233_67 | Control |
| SA094123 | 233_62 | Control |
| SA094124 | 233_70 | Control |
| SA094125 | 233_76 | Control |
| SA094126 | 233_80 | Control |
| SA094127 | 233_78 | Control |
| SA094128 | 233_42 | Control |
| SA094129 | 233_71 | Control |
| SA094130 | 233_61 | Control |
| SA094131 | 233_47 | Control |
| SA094132 | 233_51 | Control |
| SA094133 | 233_52 | Control |
| SA094134 | 233_54 | Control |
| SA094135 | 233_74 | Sepsis |
| SA094136 | 233_43 | Sepsis |
| SA094137 | 233_72 | Sepsis |
| SA094138 | 233_48 | Sepsis |
| SA094139 | 233_75 | Sepsis |
| SA094140 | 233_44 | Sepsis |
| SA094141 | 233_77 | Sepsis |
| SA094142 | 233_49 | Sepsis |
| SA094143 | 233_79 | Sepsis |
| SA094144 | 233_46 | Sepsis |
| SA094145 | 233_68 | Sepsis |
| SA094146 | 233_59 | Sepsis |
| SA094147 | 233_58 | Sepsis |
| SA094148 | 233_57 | Sepsis |
| SA094149 | 233_56 | Sepsis |
| SA094150 | 233_60 | Sepsis |
| SA094151 | 233_63 | Sepsis |
| SA094152 | 233_55 | Sepsis |
| SA094153 | 233_66 | Sepsis |
| SA094154 | 233_64 | Sepsis |
| SA094155 | 233_50 | Sepsis |
| Showing results 1 to 35 of 35 |
Collection:
| Collection ID: | CO001364 |
| Collection Summary: | WB samples (12 mL each) were collected by direct venipuncture or from an indwelling line into K2 EDTA-containing tubes. Samples were flash frozen and stored at -80C. |
| Sample Type: | Blood (whole) |
| Collection Method: | direct venipuncture or indwelling line |
| Collection Frequency: | once |
| Volumeoramount Collected: | 12mL |
| Storage Conditions: | -80℃ |
| Collection Vials: | K2 EDTA-containing Vacutainer tubes (BD 134367863; Becton-Dickinson, Franklin Lakes, NJ USA) |
| Additives: | K2 EDTA |
Treatment:
| Treatment ID: | TR001384 |
| Treatment Summary: | No treatment. Sepsis and non-sepsis controls had platelets isolated. Mitochondrial function was measured via mitochondrial oxygen consumption (mOCR) |
Sample Preparation:
| Sampleprep ID: | SP001377 |
| Sampleprep Summary: | Methanol:cholorform precipitation |
| Sampleprep Protocol ID: | WB methanol Chlorform precipitation |
| Sampleprep Protocol Filename: | 233.5.1_-_HAPS_WB_MeOHCHCl3_Precipitation |
| Processing Method: | precipitation |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Methanol chloroform precipitation |
| Extract Enrichment: | Lyophilization |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 500uL 50mM sodium phosphate buffer in D2O |
| Sample Spiking: | 4.99 mM DSS internal standard |
Analysis:
| Analysis ID: | AN002156 |
| Laboratory Name: | University of Michigan BioNMR core |
| Analysis Type: | NMR |
| Acquisition Date: | 04/05/2018, 04/09/2018, 04/10/2018 |
| Software Version: | TopSpin 4.0.7 |
| Operator Name: | Larisa Yeomans, Jae Hyun Kim |
| Num Factors: | 2 |
| Num Metabolites: | 31 |
| Units: | µM |
NMR:
| NMR ID: | NM000158 |
| Analysis ID: | AN002156 |
| Instrument Name: | Bruker 18.8 Tesla (800 MHz) NMR spectrometer ascend |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| NMR Comments: | Arrayed for water saturation frequency and 90deg. pulse width for each sample |
| Standard Concentration: | 10% (~0.5mM) |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | Triple resonance inverse detection TCI cryoprobe |
| NMR Solvent: | D20 |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming) |
| Pulse Sequence: | noesygppr1d |
| Water Suppression: | saturation at 80 Hz induced field strength |
| Pulse Width: | 5.5ms |
| Offset Frequency: | around -178Hz |
| Presaturation Power Level: | 80db |
| Chemical Shift Ref Cpd: | DSS-d6 |
| Temperature: | 25 |
| Number Of Scans: | 32 |
