Summary of Study ST001297
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000878. The data can be accessed directly via it's Project DOI: 10.21228/M86D7J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001297 |
| Study Title | High Cellulose diet impact on gut inflammation |
| Study Summary | Dietary cellulose prevents gut inflammation by modulating lipid metabolism and gut microbiota |
| Institute | Asan Medical Center |
| Last Name | Yoo |
| First Name | Hyun Ju |
| Address | 88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea |
| yoohyunju@amc.seoul.kr | |
| Phone | 82-02-3010-4029 |
| Submit Date | 2019-12-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000878 |
| Project DOI: | doi: 10.21228/M86D7J |
| Project Title: | High Cellulose diet impact on gut inflammation |
| Project Summary: | Dietary cellulose prevents gut inflammation by modulating lipid metabolism and gut microbiota |
| Institute: | Asan Medical Center |
| Last Name: | Yoo |
| First Name: | Hyun Ju |
| Address: | 88, Olympic-ro, 43-gil, Songpa-gu, Seoul 05505, South Korea |
| Email: | yoohyunju@amc.seoul.kr |
| Phone: | 82-02-3010-4029 |
Subject:
| Subject ID: | SU001371 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cellulose(%) |
|---|---|---|
| SA094168 | Low_3 | 0.3 |
| SA094169 | Low_4 | 0.3 |
| SA094170 | Low_5 | 0.3 |
| SA094171 | Low_2 | 0.3 |
| SA094172 | Low_1 | 0.3 |
| SA094173 | High_2 | 30 |
| SA094174 | High_3 | 30 |
| SA094175 | High_4 | 30 |
| SA094176 | High_5 | 30 |
| SA094177 | High_1 | 30 |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO001366 |
| Collection Summary: | Cecum contents were collected and freeze-dried for further analysis. |
| Sample Type: | Cecum |
Treatment:
| Treatment ID: | TR001386 |
| Treatment Summary: | Freeze-dried cecum contents were stored at liquid N2 until metabolomics study. |
Sample Preparation:
| Sampleprep ID: | SP001379 |
| Sampleprep Summary: | Metabolites were extracted from cecum contents using conventional liquid-liquid extraction method. (Folch method) Internal standard were added to the samples before extraction. Nonpolar and polar metabolites were obtained from organic and aqueous phases, respectively. |
Combined analysis:
| Analysis ID | AN002158 | AN002159 | AN002160 | AN002161 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Pursuit C18 (150 x 2.1mm) | Pursuit C18 (150 x 2.1mm) | Zorbax HILIC plus (100 x 2.1mm,3.5um) | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | cps | cps | cps | cps |
Chromatography:
| Chromatography ID: | CH001576 |
| Chromatography Summary: | C18_pos |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Pursuit C18 (150 x 2.1mm) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001577 |
| Chromatography Summary: | C18_neg |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Pursuit C18 (150 x 2.1mm) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001578 |
| Chromatography Summary: | HILIC_pos |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001579 |
| Chromatography Summary: | HILIC_neg |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002007 |
| Analysis ID: | AN002158 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | POSITIVE |
| MS ID: | MS002008 |
| Analysis ID: | AN002159 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002009 |
| Analysis ID: | AN002160 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | POSITIVE |
| MS ID: | MS002010 |
| Analysis ID: | AN002161 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | NEGATIVE |
