Summary of Study ST001318
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000895. The data can be accessed directly via it's Project DOI: 10.21228/M80Q38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001318 |
| Study Title | Atg32-mediated mitophagy promotes heat stress tolerance in Saccharomyces cerevisiae by sustaining spermidine and nitric oxide levels |
| Study Type | metabolomic identification |
| Study Summary | Looking for mitochondrial metabolites. |
| Institute | University of California, Davis |
| Last Name | Paglia |
| First Name | Kelly |
| Address | 451 Health Sciences Drive, Room 1313, Davis, CA, 95616, USA |
| kpaglia@ucdavis.edu | |
| Phone | 5307528129 |
| Submit Date | 2020-02-06 |
| Study Comments | West Coast Metabolomics Center - UC Davis |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2020-07-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000895 |
| Project DOI: | doi: 10.21228/M80Q38 |
| Project Title: | Atg32-mediated mitophagy promotes heat stress tolerance in Saccharomyces cerevisiae by sustaining spermidine and nitric oxide levels |
| Project Type: | metabolomics |
| Project Summary: | Looking for mitochondrial metabolites. |
| Institute: | University of California, Davis |
| Last Name: | Paglia |
| First Name: | Kelly |
| Address: | 451 Health Sciences Drive, Room 1313, Davis, CA, 95616, USA |
| Email: | kpaglia@ucdavis.edu |
| Phone: | 5307528129 |
Subject:
| Subject ID: | SU001392 |
| Subject Type: | Other |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
Factors:
Subject type: Other; Subject species: Saccharomyces cerevisiae (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA095232 | WT_30 Rep 4_004 | 1 - WT_30 |
| SA095233 | WT_30 Rep 5_005 | 1 - WT_30 |
| SA095234 | WT_30 Rep 1_001 | 1 - WT_30 |
| SA095235 | WT_30 Rep 3_003 | 1 - WT_30 |
| SA095236 | WT_30 Rep 2_002 | 1 - WT_30 |
| SA095237 | datg3_30 Rep 3_008 | 2 - datg3_30 |
| SA095238 | datg3_30 Rep 2_007 | 2 - datg3_30 |
| SA095239 | datg3_30 Rep 5_010 | 2 - datg3_30 |
| SA095240 | datg3_30 Rep 1_006 | 2 - datg3_30 |
| SA095241 | datg3_30 Rep 4_009 | 2 - datg3_30 |
| SA095242 | datg32_30 Rep 2_012 | 3 - datg32_30 |
| SA095243 | datg32_30 Rep 3_013 | 3 - datg32_30 |
| SA095244 | datg32_30 Rep 1_011 | 3 - datg32_30 |
| SA095245 | datg32_30 Rep 4_014 | 3 - datg32_30 |
| SA095246 | datg32_30 Rep 5_015 | 3 - datg32_30 |
| SA095247 | WT_37 Rep 2_017 | 4 - WT_37 |
| SA095248 | WT_37 Rep 5_020 | 4 - WT_37 |
| SA095249 | WT_37 Rep 1_016 | 4 - WT_37 |
| SA095250 | WT_37 Rep 4_019 | 4 - WT_37 |
| SA095251 | WT_37 Rep 3_018 | 4 - WT_37 |
| SA095252 | datg3_37 Rep 4_024 | 5 - datg3_37 |
| SA095253 | datg3_37 Rep 3_023 | 5 - datg3_37 |
| SA095254 | datg3_37 Rep 5_025 | 5 - datg3_37 |
| SA095255 | datg3_37 Rep 1_021 | 5 - datg3_37 |
| SA095256 | datg3_37 Rep 2_022 | 5 - datg3_37 |
| SA095257 | datg32_37 Rep 4_029 | 6 - datg32_37 |
| SA095258 | datg32_37 Rep 5_030 | 6 - datg32_37 |
| SA095259 | datg32_37 Rep 2_027 | 6 - datg32_37 |
| SA095260 | datg32_37 Rep 3_028 | 6 - datg32_37 |
| SA095261 | datg32_37 Rep 1_026 | 6 - datg32_37 |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO001387 |
| Collection Summary: | Yeast cells (WT and mutants of datg3 and datg32 were grown at 30 or 37 degrees in YPGE media to logarithmic phase, quenched with -20 chilled metanol:water (3:2), spun at 1000g for 5 minutes and the supernatant was discarded. Pellets were then saved at -80. |
| Sample Type: | Yeast cells |
Treatment:
| Treatment ID: | TR001407 |
| Treatment Summary: | Three genotypes- WT, atg3 null and atg32 null cells were grown at 30 or 37 degree for each group. (Total 6 samples per treatment). |
Sample Preparation:
| Sampleprep ID: | SP001400 |
| Sampleprep Summary: | Added 500 uL 3:3:2 and vortex before transferring to 2 mL eppendorf tube. Added another 500 uL 3:3:2 to sample tubes and vortexed before transferring to 2 mL eppendorf tube. |
Combined analysis:
| Analysis ID | AN002194 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | normalized peak height |
Chromatography:
| Chromatography ID: | CH001609 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002041 |
| Analysis ID: | AN002194 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Raw files are processed directly after data acquisition and stored as ChromaTOF-specific. |
| Ion Mode: | POSITIVE |
