Summary of Study ST001362

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000932. The data can be accessed directly via it's Project DOI: 10.21228/M8710D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001362
Study TitleCalifornia mouse fecal metabolite analysis
Study TypeEffect of BPA and genistein exposure on the fecal metabolome
Study SummaryXenoestrogens are found in plant products, such as genistein (GEN), or industrial chemicals, such as bisphenol A (BPA), present in consumer products that are also pervasive in the environment. Early exposure to such endocrine disrupting chemicals (EDC) may affect neural development by inducing direct neural effects and/or through the microbiome-gut-brain axis. To test this hypothesis, California mice (Peromyscus californicus) offspring were exposed through the maternal diet to GEN (250 mg/kg feed weight) or BPA (5 mg/kg feed weight, low dose- LD and 50 mg/kg, upper dose-UD), and dams were placed on these diets two weeks prior to breeding, throughout gestation, and lactation. Various behaviors, gut microbiome, and fecal metabolome were assessed starting at 90 days of age. The LD but not UD of BPA resulted in individuals spending more time engaging in repetitive behaviors. GEN exposed individuals were more likely to exhibit such behaviors and showed socio-communicative disturbances. BPA and GEN exposed females had increased number of metabolites involved in carbohydrate metabolism and synthesis.. Males exposed to BPA or GEN showed alterations in lysine degradation and phenylalanine and tyrosine metabolism. Current findingsindicate cause for concern that developmental exposure to BPA or GEN might affect the microbiome-gut-brain axis.
Institute
University of Missouri
DepartmentMU Metabolomics Center
Last NameSarma
First NameSaurav
Address1201 Rollins street, 243 Bond Life Science Center, University of Missouri, Columbia, MO 65211, USA
Emailsarmas@missouri.edu
Phone3366713357
Submit Date2020-04-12
Num Groups8
Total Subjects78
Num Males37
Num Females41
Raw Data AvailableYes
Raw Data File Type(s)baf
Analysis Type DetailGC-MS/LC-MS
Release Date2020-05-13
Release Version1
Saurav Sarma Saurav Sarma
https://dx.doi.org/10.21228/M8710D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000932
Project DOI:doi: 10.21228/M8710D
Project Title:California mouse fecal metabolite analysis
Project Summary:Xenoestrogens are found in plant products, such as genistein (GEN), or industrial chemicals, such as bisphenol A (BPA), present in consumer products that are also pervasive in the environment. Early exposure to such endocrine disrupting chemicals (EDC) may affect neural development by inducing direct neural effects and/or through the microbiome-gut-brain axis. To test this hypothesis, California mice (Peromyscus californicus) offspring were exposed through the maternal diet to GEN (250 mg/kg feed weight) or BPA (5 mg/kg feed weight, low dose- LD and 50 mg/kg, upper dose-UD), and dams were placed on these diets two weeks prior to breeding, throughout gestation, and lactation. Various behaviors, gut microbiome, and fecal metabolome were assessed starting at 90 days of age. The LD but not UD of BPA resulted in individuals spending more time engaging in repetitive behaviors. GEN exposed individuals were more likely to exhibit such behaviors and showed socio-communicative disturbances. BPA and GEN exposed females had increased number of metabolites involved in carbohydrate metabolism and synthesis.. Males exposed to BPA or GEN showed alterations in lysine degradation and phenylalanine and tyrosine metabolism. Current findingsindicate cause for concern that developmental exposure to BPA or GEN might affect the microbiome-gut-brain axis.
Institute:University of Missouri
Department:MU Metabolomics Center
Last Name:Sarma
First Name:Saurav
Address:1201 Rollins Street, 243 Bond Life Sciences Center, University of Missouri, Columbia, MO, 65201, USA
Email:sarmas@missouri.edu
Phone:5738825596

Subject:

Subject ID:SU001436
Subject Type:Mammal
Subject Species:Peromyscus Californicus
Taxonomy ID:42520
Age Or Age Range:PND90
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Peromyscus Californicus (Factor headings shown in green)

mb_sample_id local_sample_id Exposure Gender
SA0989891642-F-AINAIN F
SA0989901587-F-AINAIN F
SA0989911765-F-AINAIN F
SA0989921702-F-AINAIN F
SA0989931622-F-AINAIN F
SA0989941618-F-AINAIN F
SA0989951745-F-AINAIN F
SA0989961626-F-AINAIN F
SA0989971672-F-AINAIN F
SA0989981561-F-AINAIN F
SA0989991609-M-AINAIN M
SA0990001560-M-AINAIN M
SA0990011744-M-AINAIN M
SA0990021619-M-AINAIN M
SA0990031627-M-AINAIN M
SA0990041632-M-AINAIN M
SA0990051620-M-AINAIN M
SA0990061623-M-AINAIN M
SA0990071764-M-AINAIN M
SA0990082040-F-GENGEN F
SA0990091940-F-GENGEN F
SA0990101829-F-GENGEN F
SA0990111926-F-GENGEN F
SA0990122022-F-GENGEN F
SA0990131925-F-GENGEN F
SA0990141788-F-GENGEN F
SA0990151911-F-GENGEN F
SA0990161763-F-GENGEN F
SA0990171912-F-GENGEN F
SA0990182041-M-GENGEN M
SA0990192021-M-GENGEN M
SA0990201830-M-GENGEN M
SA0990211884-M-GENGEN M
SA0990221975-M-GENGEN M
SA0990231738-M-GENGEN M
SA0990241993-M-GENGEN M
SA0990251819-M-GENGEN M
SA0990261965-M-GENGEN M
SA0990271808-M-GENGEN M
SA0990282009-F-LD BPALD BPA F
SA0990291923-F-LD BPALD BPA F
SA0990301953-F-LD BPALD BPA F
SA0990312005-F-LD BPALD BPA F
SA0990321991-F-LD BPALD BPA F
SA0990331848-F-LD BPALD BPA F
SA0990342001-F-LD BPALD BPA F
SA0990351791-F-LD BPALD BPA F
SA0990361914-F-LD BPALD BPA F
SA0990372007-F-LD BPALD BPA F
SA0990381854-F-LD BPALD BPA F
SA0990391992-M-LD BPALD BPA M
SA0990402010-M-LD BPALD BPA M
SA0990411910-M-LD BPALD BPA M
SA0990421913-M-LD BPALD BPA M
SA0990432008-M-LD BPALD BPA M
SA0990442056-M-LD BPALD BPA M
SA0990452049-M-LD BPALD BPA M
SA0990462019-M-LD BPALD BPA M
SA0990471689-F-UD BPAUD BPA F
SA0990481652-F-UD BPAUD BPA F
SA0990491827-F-UD BPAUD BPA F
SA0990501682-F-UD BPAUD BPA F
SA0990511614-F-UD BPAUD BPA F
SA0990522038-F-UD BPAUD BPA F
SA0990532004-F-UD BPAUD BPA F
SA0990541607-F-UD BPAUD BPA F
SA0990551612-F-UD BPAUD BPA F
SA0990562006-F-UD BPAUD BPA F
SA0990571800-M-UD BPAUD BPA M
SA0990581690-M-UD BPAUD BPA M
SA0990591651-M-UD BPAUD BPA M
SA0990601747-M-UD BPAUD BPA M
SA0990611615-M-UD BPAUD BPA M
SA0990621613-M-UD BPAUD BPA M
SA0990632037-M-UD BPAUD BPA M
SA0990641688-M-UD BPAUD BPA M
SA0990651606-M-UD BPAUD BPA M
SA0990662028-M-UD BPAUD BPA M
Showing results 1 to 78 of 78

Collection:

Collection ID:CO001431
Collection Summary:Fecal boli were collected at PND 90, snap frozen in LN2, stored in -80 freezer until processing
Sample Type:Feces
Collection Method:Snap freezen in LN2 right after excretion
Collection Location:Animal Science Research Center, University of Missouri, Columbia
Collection Frequency:1 time from each animal
Volumeoramount Collected:3-4 boli
Storage Conditions:-20℃
Collection Vials:2mL vials
Storage Vials:2mL vials

Treatment:

Treatment ID:TR001451
Treatment Summary:2 weeks before breeding and during gestation, dams were fed estrogen-free AIN 93G or AIN 93G diet containing LD BPA, UD BPA or genistein untill one male and one female pups weaned. F1 pups fed on AIN 93G diet after weaning.
Treatment:diet
Treatment Compound:Genistein and Bisphenol A
Treatment Route:oral
Treatment Dose:Genistein: 250 mg/kg feed weight; BPA: 5 mg/kg feed weight for LD BPA and 50 mg/kg feed weight for UD BPA group
Treatment Doseduration:2 weeks before breeding and untill one male and one female pups weaned.
Treatment Vehicle:AIN control diet
Animal Endp Tissue Coll List:Fecal

Sample Preparation:

Sampleprep ID:SP001444
Sampleprep Summary:Fecal samples were snap frozen in liqud nitrogen and no further preparation
Processing Method:Fecal samples were lyophilized for 24 h before weighing
Processing Storage Conditions:-20℃
Extraction Method:For LCMS analysis lyophilized fecal sample was extracted with 80% Methanol containg 18 µg/ml of umbelliferon, sonicated and vortexed 20 seconds each, shaken at 140 rpmfor 2 h. For GCMS-polar analysis samples were extracted with water, containing 25 μg/ml ribitol, vortex for 20 seconds, sonicated for 20 seconds, incubated at 50C for 1h. For GCMS-nonpolar samples were extracted with chloroform, containing 10 μl/ml docosanol, vortex for 20 seconds, sonicated for 20 second, incubated at 50C for 1h.
Extract Enrichment:Solution were centrifuged at 3000g for 40 min then extracted 0.5 mL of supernatant for LCMS, 1 mL each of supernatant was collected for GCMS-polar and GCMS-nonpolar analysis.
Extract Storage:-20℃
Sample Resuspension:For GCMS analysis extracts dried under nitrogen flow and storred at -20C then resuspended in 25 μl of pyridine containing 15 mg/ml methoxyamine hydrochloride
Sample Derivatization:GCMS samples were derivatized with 25 μl MSTFA (N-methyl-N-(trimethyl-silyl)trifluoroacetamide) + 1% TMCS (chlorotrimethylsilane)
Sample Spiking:Umbelliferon, ribitol and docosanol were used as internal standard for LCMS, GCMS-polar and GCMS-nonpolar respectively.

Combined analysis:

Analysis ID AN002266 AN002267 AN002268
Analysis type MS MS MS
Chromatography type Reversed phase GC GC
Chromatography system Waters Acquity I-Class Agilent 6890N Agilent 6890N
Column Waters Acquity BEH C18 (150 x 2mm,1.7um) DB-5MS (J&W Scientific) 60m DB-5MS (J&W Scientific) 60m
MS Type ESI EI EI
MS instrument type QTOF Single quadrupole Single quadrupole
MS instrument name Bruker maXis Impact qTOF Agilent 5973 Agilent 5973
Ion Mode POSITIVE POSITIVE POSITIVE
Units intensity intensity intensity

Chromatography:

Chromatography ID:CH001665
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity BEH C18 (150 x 2mm,1.7um)
Column Pressure:>11000 psi
Column Temperature:60
Flow Gradient:Solvent B (acetonitrile) was increased from 5% to 70% over 30 min, then to 95% over 3 min, held at 95% for 3 min, then returned to 5% for equilibrium
Flow Rate:0.560 ml/min
Internal Standard:Umbelliferon
Retention Time:1 to 35 min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Analytical Time:40 min
Chromatography Type:Reversed phase
  
Chromatography ID:CH001666
Chromatography Summary:5:1 split for GCMS-polar injection with 1uL injection volume.
Instrument Name:Agilent 6890N
Column Name:DB-5MS (J&W Scientific) 60m
Column Pressure:variable
Column Temperature:variable between 80-315
Flow Rate:1mL/Min of Helium gas
Internal Standard:Ribitol
Retention Time:9 to 61 min
Sample Injection:5:1 split injection with 1uL injection volume
Analytical Time:61 min
Chromatography Type:GC
  
Chromatography ID:CH001667
Chromatography Summary:1:1 split for GCMS-nonpolar injection with 1uL injection volume.
Instrument Name:Agilent 6890N
Column Name:DB-5MS (J&W Scientific) 60m
Column Pressure:variable
Column Temperature:variable between 80-315
Flow Rate:1mL/Min of Helium gas
Internal Standard:Docosanol
Retention Time:9 to 61 min
Sample Injection:1:1 split injection with 1uL injection volume
Analytical Time:61 min
Chromatography Type:GC

MS:

MS ID:MS002110
Analysis ID:AN002266
Instrument Name:Bruker maXis Impact qTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Bruker Hystar and otof for acquisition and Metaboscape 4.0 and MS-DIAL for data analysis
Ion Mode:POSITIVE
Collision Energy:Varied, depended on procursor m/z
Collision Gas:Nitrogen
Dry Gas Flow:12 l/min
Dry Gas Temp:250
Fragmentation Method:CID
Gas Pressure:43.5 psi of nebulizer gas
Dataformat:.d
Nebulizer:Nitrogen
  
MS ID:MS002111
Analysis ID:AN002267
Instrument Name:Agilent 5973
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:Agilent Chemstation software for data acquisition and AMDIS and MetIdea for data analysis
Ion Mode:POSITIVE
Helium Flow:1mL/min
Ion Source Temperature:230
Ionization Energy:70ev
Reagent Gas:Helium
Source Temperature:230
Dataformat:.d
  
MS ID:MS002112
Analysis ID:AN002268
Instrument Name:Agilent 5973
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:Agilent Chemstation software for data acquisition and AMDIS and MetIdea for data analysis
Ion Mode:POSITIVE
Helium Flow:1mL/min
Ion Source Temperature:230
Ionization Energy:70ev
Reagent Gas:Helium
Source Temperature:230
Dataformat:.d
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