Summary of study ST001377

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000942. The data can be accessed directly via it's Project DOI: 10.21228/M8XM5C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001377
Study TitleStirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells (hPSCs)
Study SummaryAlthough cell therapies require large numbers of quality-controlled hPSCs, existing technologies are limited in their ability to efficiently grow and scale stem cells. We report here that cell-state conversion from primed-to-naïve pluripotency enhances the biomanufacturing of hPSCs. Naïve hPSCs exhibit superior growth kinetics and aggregate formation characteristics in stirred suspension bioreactors compared to their primed counterparts. Moreover, we demonstrate the role of the bioreactor mechanical environment in the maintenance of naïve pluripotency, through transcriptomic enrichment of mechano-sensing signaling for cells in the bioreactor along with a decrease in expression of lineage-specific and primed pluripotency hallmarks. Bioreactor-cultured, naïve hPSCs express epigenetic regulatory transcripts associated with naïve pluripotency, and display hallmarks of X-chromosome reactivation. They exhibit robust production of naïve pluripotency metabolites and display reduced expression of primed pluripotency cell surface markers. We also show that these cells retain the ability to undergo targeted differentiation into beating cardiomyocytes, hepatocytes, and neural rosettes. They additionally display faster kinetics of teratoma formation compared to their primed counterparts. Naïve bioreactor hPSCs also retain structurally stable chromosomes. Our research corroborates that converting hPSCs to the naïve state enhances hPSC manufacturing and indicates a potentially important role for the bioreactor’s mechanical environment in maintaining naïve pluripotency.
Institute
University of Calgary
DepartmentBiochemistry and Molecular Biology
LaboratoryStem Cell Research
Last NameRohani
First NameLeili
Address405J, 1919 University Drive, NW
Emailleili.rohanisarvesta@ucalgary.ca
Phone+1 5879681647
Submit Date2020-05-08
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2020-07-20
Release Version1
Leili Rohani Leili Rohani
https://dx.doi.org/10.21228/M8XM5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000942
Project DOI:doi: 10.21228/M8XM5C
Project Title:Inferring metabolism dynamics between naïve and primed human pluripotent stem cells in the stirred suspension bioreactor
Project Type:Targeted and untargeted MS
Project Summary:We used high-resolution LC-MS targeted metabolomics to screen for extracellular metabolites in the media of bioreactor-cultured, naïve and primed human pluripotent stem cells.
Institute:University of Calgary
Department:Biochemistry and Molecular Biology
Laboratory:Stem Cell Research
Last Name:Rohani
First Name:Leili
Address:405J, 1919 University Drive, NW
Email:leili.rohanisarvesta@ucalgary.ca
Phone:5879681647
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