Summary of Study ST001395

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000893. The data can be accessed directly via it's Project DOI: 10.21228/M8869V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001395
Study Title	Time-course experiment of Microchloropsis gaditana cells supplemented with CO2 (part-II)
Study Type	Time-course experiment
Study Summary	Experiments were conducted with Microchloropsis gaditana supplemented with very-low CO2 and high CO2. Sampling was done on the following time points: Day 3, 6 and 9.
Institute	International Centre for Genetic Engineering and Biotechnology
Department	Integrative Biology
Laboratory	Omics of Algae
Last Name	Jutur
First Name	Pannaga Pavan
Address	2nd Floor, New Building, ICGEB, Aruna Asaf Ali Marg, New Delhi - 110067
Email	jppavan@icgeb.res.in
Phone	+91 11 26741358
Submit Date	2020-06-08
Study Comments	Former name of species: Nannochloropsis gaditana Lubián
Raw Data Available	Yes
Raw Data File Type(s)	mzdata.xml
Analysis Type Detail	LC-MS
Release Date	2020-07-05
Release Version	1

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Project:

Project ID:	PR000893
Project DOI:	doi: 10.21228/M8869V
Project Title:	Carbon Partitioning Metabolomic Studies
Project Type:	MS Qualitative Analysis
Project Summary:	Qualitative metabolomic studies in response to very-low CO2 and high CO2 in Microchloropsis gaditana
Institute:	International Centre for Genetic Engineering and Biotechnology
Department:	Integrative Biology
Laboratory:	Omics of Algae
Last Name:	Jutur
First Name:	Pannaga Pavan
Address:	2nd Floor, New Building, ICGEB, Aruna Asaf Ali Marg, New Delhi - 110067
Email:	jppavan@icgeb.res.in
Phone:	+91 11 26741358
Project Comments:	http://www.icgeb.res.in/

Subject:

Subject ID:	SU001469
Subject Type:	Other
Subject Species:	Nannochloropsis gaditana
Taxonomy ID:	72520
Genotype Strain:	NIES 2587
Species Group:	Algae

Factors:

Subject type: Other; Subject species: Nannochloropsis gaditana (Factor headings shown in green)

mb_sample_id	local_sample_id	Condition	Sample Collection (in Days)
SA113496	VLC3_1	Control	3
SA113499	VLC3_2	Control	3
SA113501	VLC3_3	Control	3
SA113497	VLC6_3	Control	6
SA113500	VLC6_1	Control	6
SA113502	VLC6_2	Control	6
SA113494	VLC9_2	Control	9
SA113495	VLC9_3	Control	9
SA113498	VLC9_1	Control	9
SA113507	HC3_1	Treatment	3
SA113508	HC3_2	Treatment	3
SA113509	HC3_3	Treatment	3
SA113506	HC6_3	Treatment	6
SA113510	HC6_1	Treatment	6
SA113511	HC6_2	Treatment	6
SA113503	HC9_1	Treatment	9
SA113504	HC9_2	Treatment	9
SA113505	HC9_3	Treatment	9

Showing results 1 to 18 of 18

Showing results 1 to 18 of 18

Collection:

Collection ID:	CO001464
Collection Summary:	Marine microalgae Microchloropsis gaditana NIES 2587 is procured from Microbial Culture Collection, National Institute for Environmental Studies (NIES), Tsukuba, Japan. The strain was grown in minimal medium F/2 (Guillard and Ryther, 1962) under a light regime of 16:8 h and an illumination of 150 µmol m−2 s−1 photosynthetically active radiation (PAR) in a multi-cultivator MC 1000-OD (Photon Systems Instruments, Czech Republic) with a flow rate of 800 mL min-1 with continuous bubbling of air at 24 °C.
Sample Type:	Algae

Treatment:

Treatment ID:	TR001484
Treatment Summary:	Microchloropsis gaditana cells were grown in a Multicultivator in the presence of very-low CO2 (300 ppm) and high CO2 (30,000 ppm) for 9 days with an illumination intensity of 150 uE.
Treatment Compound:	CO2

Sample Preparation:

Sampleprep ID:	SP001477
Sampleprep Summary:	Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis.

Sampleprep ID:

SP001477

Sampleprep Summary:

Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis.

Chromatography:

Chromatography ID:	CH001710
Chromatography Summary:	GC-triple quadrupole analysis was performed on an HP-5 gas chromatograph with standard liners containing glass wool in split mode (1:5) at 250°C injector temperature. The GC was operated at constant flow of 1 ml/min helium on a 30-m, 0.25-mm i.d., 0.25-μm HP-5 column, a start temperature of 60°C, 3 min isothermal, temperature ramping by 5°C/min to 180°C, 3 min isothermal and finally temperature ramping of 10°C/min to 310°C.
Instrument Name:	Agilent 7890A
Column Name:	Agilent HP5-MS (30m x 0.25mm, 0.25 um)
Chromatography Type:	GC

Analysis:

Analysis ID:	AN002334
Analysis Type:	MS
Chromatography ID:	CH001710
Num Factors:	6
Num Metabolites:	31
Units:	area