Summary of Study ST001399

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000959. The data can be accessed directly via it's Project DOI: 10.21228/M8QX3V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001399
Study Title	Activation of ectopic olfactory receptor 544 induces GLP-1 secretion, alters gut microbiome, and improves intestinal permeability.
Study Type	Fecal metabolome
Study Summary	Metabolome data set from mouse fecal samples Group - WT_AZA: fecal samples from wild type mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - WT_DW: fecal samples from wild type mouse fed with high fat diet Group - KO_AZA: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - KO_DW: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet
Institute	Korea University
Last Name	Kim
First Name	Jungyeon
Address	145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South
Email	kim131812@korea.ac.kr
Phone	821082248015
Submit Date	2020-06-07
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2020-06-30
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000959
Project DOI:	doi: 10.21228/M8QX3V
Project Title:	Activation of ectopic olfactory receptor 544 induces GLP-1 secretion, alters gut microbiome, and improves intestinal permeability
Project Summary:	Metabolome data from the mouse fecal samples. Mouse were fed with high fat diet or high fat diet with azelaic acid (0.05%; w/w) Fecal samples were analyzed by GC/TOF MS.
Institute:	Graduate school of Korea University
Last Name:	Kim
First Name:	Jungyeon
Address:	145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South
Email:	kim131812@korea.ac.kr
Phone:	821082248015

Subject:

Subject ID:	SU001473
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	Samtako (Gyeonggi-do, Korea)
Gender:	Male

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Genotype	Treatment
SA113588	KO_DW_4	Olfr544 KO mouse	HFD
SA113589	KO_DW_2	Olfr544 KO mouse	HFD
SA113590	KO_DW_1	Olfr544 KO mouse	HFD
SA113591	KO_DW_5	Olfr544 KO mouse	HFD
SA113592	KO_DW_3	Olfr544 KO mouse	HFD
SA113593	KO_DW_6	Olfr544 KO mouse	HFD
SA113594	KO_DW_8	Olfr544 KO mouse	HFD
SA113595	KO_DW_7	Olfr544 KO mouse	HFD
SA113596	KO_AZA_3	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113597	KO_AZA_2	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113598	KO_AZA_4	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113599	KO_AZA_7	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113600	KO_AZA_1	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113601	KO_AZA_6	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113602	KO_AZA_5	Olfr544 KO mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113603	WT_DW_8	WT mouse	HFD
SA113604	WT_DW_3	WT mouse	HFD
SA113605	WT_DW_2	WT mouse	HFD
SA113606	WT_DW_4	WT mouse	HFD
SA113607	WT_DW_1	WT mouse	HFD
SA113608	WT_DW_7	WT mouse	HFD
SA113609	WT_DW_6	WT mouse	HFD
SA113610	WT_DW_5	WT mouse	HFD
SA113611	WT_AZA_4	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113612	WT_AZA_2	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113613	WT_AZA_5	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113614	WT_AZA_3	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113615	WT_AZA_7	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113616	WT_AZA_1	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113617	WT_AZA_8	WT mouse	HFD + Azelaic acid 0.05 % (w/w)
SA113618	WT_AZA_6	WT mouse	HFD + Azelaic acid 0.05 % (w/w)

Showing results 1 to 31 of 31

Showing results 1 to 31 of 31

Collection:

Collection ID:	CO001468
Collection Summary:	Fecal samples were thawed on ice, and 50 mg of each sample was transferred to a fresh 1.5-mL Eppendorf tube. Then, 1 mL of pure methanol (Merck Millipore, Burlington, MA, USA) was added to each sample. The mixture was homogenised using a needle, sonicated for 30 min, vortexed for 10 min, and centrifuged at 16,100 × g for 10 min at 4°C. The supernatant was filtered by using a syringe filter (0.20-µm pore size; Thermo Fisher Scientific, Waltham, MA, USA). Aliquots of 500 μL of the supernatant were dried under vacuum at room temperature.
Sample Type:	Feces

Treatment:

Treatment ID:	TR001488
Treatment Summary:	The mice were fed on 60% high fat diet (HFD) for 4 weeks and subsequently randomly assigned into the test or vehicle group. HFD used in this study is rodent diet containing 60% of calories from fat (D12492, Research Diets, Inc). The mice in the test group were fed with a HFD and 0.05% (w/w) AzA for 6 weeks or a HFD and orally administered AzA (50 mg/kg body weight) for 8 weeks. The former feeding was to confirm antiobesogenic effects of AzA in diet and the latter was to investigate microbiome and metabolomic analyses. The mice in the vehicle group were administered ddH2O. The body weight and food intake of the mice were measured twice a week. Mice were fasted overnight before being sacrificed.

Treatment ID:

TR001488

Treatment Summary:

The mice were fed on 60% high fat diet (HFD) for 4 weeks and subsequently randomly assigned into the test or vehicle group. HFD used in this study is rodent diet containing 60% of calories from fat (D12492, Research Diets, Inc). The mice in the test group were fed with a HFD and 0.05% (w/w) AzA for 6 weeks or a HFD and orally administered AzA (50 mg/kg body weight) for 8 weeks. The former feeding was to confirm antiobesogenic effects of AzA in diet and the latter was to investigate microbiome and metabolomic analyses. The mice in the vehicle group were administered ddH2O. The body weight and food intake of the mice were measured twice a week. Mice were fasted overnight before being sacrificed.

Sample Preparation:

Sampleprep ID:	SP001481
Sampleprep Summary:	Methoximation and silylation were performed for qualification and relative quantification of metabolites using GC/TOF–MS, For methoximation, 10 µL of 40 mg/mL methoxyamine hydrochloride in pyridine (Sigma-Aldrich) was added to the dried sample, and the mixture was incubated at 30°C for 90 minutes. For silylation, 45 µL of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) was added to the mixture, and the mixture was incubated at 37°C for 30 minutes.

Sampleprep ID:

SP001481

Sampleprep Summary:

Methoximation and silylation were performed for qualification and relative quantification of metabolites using GC/TOF–MS, For methoximation, 10 µL of 40 mg/mL methoxyamine hydrochloride in pyridine (Sigma-Aldrich) was added to the dried sample, and the mixture was incubated at 30°C for 90 minutes. For silylation, 45 µL of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) was added to the mixture, and the mixture was incubated at 37°C for 30 minutes.

Combined analysis:

Analysis ID	AN002339
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890B
Column	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type	EI
MS instrument type	GC-TOF
MS instrument name	Leco Pegasus HT TOF
Ion Mode	POSITIVE
Units	Arbtrary unit

Chromatography:

Chromatography ID:	CH001714
Instrument Name:	Agilent 7890B
Column Name:	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:	GC

MS:

MS ID:	MS002181
Analysis ID:	AN002339
Instrument Name:	Leco Pegasus HT TOF
Instrument Type:	GC-TOF
MS Type:	EI
MS Comments:	Mass spectra were recorded over a mass range of 85–500 m/z at an acquisition rate of 16 spectra/s. The temperatures of the ion source and transfer line of the TOF–MS were 250°C and 280°C, respectively. The electron ionisation energy was 70 eV.Chroma TOF Software C version (LECO) was used for the detection and deconvolution of peaks and mass spectra. BinBase was used for the identification and relative quantification of metabolites from preprocessed data by matching mass spectra and retention indices of peaks with the customised reference mass spectral and retention index libraries, acquired using authentic standards with identical data acquisition parameters for the Fiehn and NIST libraries. Peaks with mass spectral similarities above 700, in comparison to their authentic standards in the libraries, were regarded as authentic metabolites. Intensities of metabolites were reported as peak heights of their unique ion intensities. For management of missing values, the lowest background intensity was subtracted from the intensity of the quantified ion in its retention time region ± 5s, using MZmine.
Ion Mode:	POSITIVE