Summary of Study ST001437

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000988. The data can be accessed directly via it's Project DOI: 10.21228/M80680 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001437
Study TitleSub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - EBC
Study SummaryThe human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. The first set of samples tested for this approach included exhaled breath condensates (EBC) collected from cystic fibrosis (CF) patients with impaired glucose tolerance to study the metabolome changes before and after the oral glucose tolerance test.
Institute
Georgia Institute of Technology
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW
Emailfernandez@gatech.edu
Phone(404) 385-4432
Submit Date2020-07-29
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailMS(Dir. Inf.)
Release Date2020-09-14
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M80680
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000988
Project DOI:doi: 10.21228/M80680
Project Title:Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples
Project Summary:The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. Samples tested for this approach included exhaled breath condensates (EBC) collected from cystic fibrosis (CF) patients as well as in vitro-cultured human mesenchymal stromal cells (MSCs). Both test samples were only available in minimum amounts. Experiments showed that picoliter-volume spray pulses sufficed to generate high-quality spectral fingerprints, which increased the information density produced per unit sample volume. This TENGi nanoESI strategy has the potential to fill in the gap in metabolomics where liquid chromatography-MS-based analyses cannot be applied. Our method could open up new avenues for future investigations into understanding metabolic changes caused by diseases or external stimuli.
Institute:Georgia Institute of Technology
Last Name:Fernandez
First Name:Facundo
Address:901 Atlantic Dr NE, Atlanta, GA, 30332, USA
Email:fernandez@gatech.edu
Phone:404-385-4432

Subject:

Subject ID:SU001511
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA122018Blank02Blank
SA122019Blank03Blank
SA12202017B03Post
SA12202117B02Post
SA1220228B03Post
SA1220239B03Post
SA12202416B02Post
SA12202511B01Post
SA1220269B01Post
SA1220278B02Post
SA12202813B01Post
SA12202916B01Post
SA12203012B01Post
SA12203111B02Post
SA12203213B03Post
SA12203312B02Post
SA12203414B01Post
SA12203515B02Post
SA12203615B01Post
SA12203714B02Post
SA1220382A03Pre
SA1220392A02Pre
SA12204017A02Pre
SA1220419A01Pre
SA12204217A01Pre
SA1220439A02Pre
SA1220448A03Pre
SA1220458A02Pre
SA12204613A03Pre
SA12204711A01Pre
SA12204810A02Pre
SA12204916A02Pre
SA12205010A01Pre
SA12205112A01Pre
SA12205211A02Pre
SA12205312A02Pre
SA12205416A01Pre
SA12205514A01Pre
SA12205614A02Pre
SA12205713A01Pre
SA122058QC_D02QC
SA122059QC_E02QC
SA122060QC_E03QC
SA122061QC_D01QC
SA122062QC_A01QC
SA122063QC_A03QC
SA122064QC_B02QC
SA122065QC_B03QC
SA122066QC_C01QC
SA122067QC_C02QC
Showing results 1 to 50 of 50

Collection:

Collection ID:CO001506
Collection Summary:EBC from 11 CF patients with abnormalities in glucose homeostasis (as defined by having prediabetes/impaired glucose tolerance [CF IGT] on an oral glucose tolerance test) was collected both fasting and 2 hours following ingestion of a glucose drink. They were termed as “Pre” and “Post”, respectively. Of these 11 subjects, one did not have an adequate sample for the “Pre” and another did not have a sample for the “Post” study giving a total of 20 samples analyzed (one of then failed to purduce enough features as others and was regarded as an outlier and removed). EBC sample collection followed the guidelines approved by the Georgia Institute of Technology and the Emory University Institutional Review Boards (approval number IRB00000372). Samples were collected with an R-Tube collector (Respiratory Research, Inc., Austin, TX, USA). After collection, samples were immediately frozen at -80℃ until processed.
Sample Type:Exhaled Breath condensate

Treatment:

Treatment ID:TR001526
Treatment Summary:A total of 20 EBC samples (10 for each group), each 50 µL in volume before concentration, were phenotyped. A pooled sample, which was used as a QC, was prepared by taking 5 µL from each EBC sample and mixing the aliquots together. Then, the 20 samples, together with the pooled QC sample and the blank sample (containing only ultrapure water) were lyophilized at -40 C and 100 mTorr for 24h in a VirTis Benchtop freeze-drier (LP Industries, Stone Ridge, NY, USA). Residues were then reconstituted in 9 μL of methanol/water 1:9 (v:v) with 1×10-6 M 13C-tyrosine spiked in. This resulted in a 5-fold concentration factor.

Sample Preparation:

Sampleprep ID:SP001519
Sampleprep Summary:A total of 20 EBC samples (10 for each group), each 50 µL in volume before concentration, were phenotyped. A pooled sample, which was used as a QC, was prepared by taking 5 µL from each EBC sample and mixing the aliquots together. Then, the 20 samples, together with the pooled QC sample and the blank sample (containing only ultrapure water) were lyophilized at -40 C and 100 mTorr for 24h in a VirTis Benchtop freeze-drier (LP Industries, Stone Ridge, NY, USA). Residues were then reconstituted in 9 μL of methanol/water 1:9 (v:v) with 1×10-6 M 13C-tyrosine spiked in. This resulted in a 5-fold concentration factor.

Combined analysis:

Analysis ID AN002401
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Synapt G2
Ion Mode NEGATIVE
Units Normalized Intensity (Intensity ratio against internal standard signal))

Chromatography:

Chromatography ID:CH001764
Instrument Name:none
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002242
Analysis ID:AN002401
Instrument Name:Waters Synapt G2
Instrument Type:QTOF
MS Type:ESI
MS Comments:Negative ion mode in the 50-750 m/z range was used for experiments Detailed data process see attached Method file
Ion Mode:NEGATIVE
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