Summary of Study ST001632

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001043. The data can be accessed directly via it's Project DOI: 10.21228/M8W41Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST001632
Study TitleLipidomics of Ginseng Total Secondary Saponins on Myocardial Ischemia Rat
Study SummaryCardioprotective Effect of Ginseng Total Secondary Saponins on Myocardial Ischemia Rat via Regulating Disturbed Lipids Based on Lipidomics.
Institute
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
Last NameWang
First NameZhe
AddressNo.2 nanwei street, xichen district, Beijing, China
Emailwangzhe@imm.ac.cn
Phone+861083154880
Submit Date2020-11-22
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-01-02
Release Version1
Zhe Wang Zhe Wang
https://dx.doi.org/10.21228/M8W41Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001043
Project DOI:doi: 10.21228/M8W41Q
Project Title:Lipidomics of Ginseng Total Secondary Saponins on Myocardial Ischemia Rat
Project Summary:Cardioprotective Effect of Ginseng Total Secondary Saponins on Myocardial Ischemia Rat via Regulating Disturbed Lipids Based on Lipidomics.
Institute:Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
Last Name:Wang
First Name:Zhe
Address:No.2 nanwei street, xichen district, Beijing, China
Email:wangzhe@imm.ac.cn
Phone:+861083154880

Subject:

Subject ID:SU001709
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Sprague-Dawley
Age Or Age Range:6 weeks-age
Weight Or Weight Range:200 ± 10
Gender:Male
Animal Animal Supplier:Vital River Laboratories Co., Ltd (Beijng, China)
Animal Housing:25 ± 2℃, 50±5% relative humidity
Animal Light Cycle:12 h light/12 h dark photoperiod

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id class Tissue
SA1380521-C5-HLControl heart
SA1380531-A3-N-HLControl heart
SA1380541-A5-N-HLControl heart
SA1380551-B2-HLControl heart
SA1380561-B1-HLControl heart
SA1380571-A3-HLControl heart
SA1380581-A5-HLControl heart
SA1380591-B1-N-HLControl heart
SA1380601-C5-N-HLControl heart
SA1380611-B1-HSControl heart
SA1380621-B2-HSControl heart
SA1380631-B5-HSControl heart
SA1380641-A5-HSControl heart
SA1380651-A3-HSControl heart
SA1380661-A1-HLControl heart
SA1380671-A1-HSControl heart
SA1380681-B2-N-HLControl heart
SA1380691-A1-N-HLControl heart
SA1380701-A3-PLControl plasma
SA1380711-A2-PSControl plasma
SA1380721-B3-PLControl plasma
SA1380731-A3-N-PLControl plasma
SA1380741-B2-PLControl plasma
SA1380751-B5-PLControl plasma
SA1380761-C1-PLControl plasma
SA1380771-A5-N-PLControl plasma
SA1380781-A5-PSControl plasma
SA1380791-A3-PSControl plasma
SA1380801-B2-PSControl plasma
SA1380811-B5-PSControl plasma
SA1380821-A5-PLControl plasma
SA1380831-B5-N-PLControl plasma
SA1380841-B3-N-PLControl plasma
SA1380851-B3-PSControl plasma
SA1380861-B2-N-PLControl plasma
SA1380871-C1-N-PLControl plasma
SA1380888-C5-HLGTSS heart
SA1380898-A4-HLGTSS heart
SA1380908-B2-HLGTSS heart
SA1380918-B4-HLGTSS heart
SA1380928-B2-N-HLGTSS heart
SA1380938-B4-N-HLGTSS heart
SA1380948-C5-N-HLGTSS heart
SA1380958-A2-HLGTSS heart
SA1380968-A4-N-HLGTSS heart
SA1380978-A2-N-HLGTSS heart
SA1380988-A1-N-HLGTSS heart
SA1380998-A1-HLGTSS heart
SA1381008-A2-HSGTSS heart
SA1381018-A1-HSGTSS heart
SA1381028-A4-HSGTSS heart
SA1381038-B2-HSGTSS heart
SA1381048-B3-HSGTSS heart
SA1381058-C5-HSGTSS heart
SA1381068-C2-N-PLGTSS plasma
SA1381078-C4-PLGTSS plasma
SA1381088-C4-N-PLGTSS plasma
SA1381098-C1-N-PLGTSS plasma
SA1381108-A1-N-PLGTSS plasma
SA1381118-B2-PSGTSS plasma
SA1381128-B3-PSGTSS plasma
SA1381138-A2-N-PLGTSS plasma
SA1381148-B3-N-PLGTSS plasma
SA1381158-C2-PLGTSS plasma
SA1381168-B3-PLGTSS plasma
SA1381178-C2-PSGTSS plasma
SA1381188-A1-PLGTSS plasma
SA1381198-C1-PLGTSS plasma
SA1381208-A2-PLGTSS plasma
SA1381218-A2-PSGTSS plasma
SA1381228-C4-PSGTSS plasma
SA1381238-C5-PSGTSS plasma
SA1381242-A3-HSModel heart
SA1381252-A2-HSModel heart
SA1381262-A4-HSModel heart
SA1381272-C5-HSModel heart
SA1381282-A1-HSModel heart
SA1381292-A5-HSModel heart
SA1381302-B2-N-HLModel heart
SA1381312-A1-N-HLModel heart
SA1381322-A1-HLModel heart
SA1381332-A5-N-HLModel heart
SA1381342-A5-HLModel heart
SA1381352-B1-HLModel heart
SA1381362-B3-HLModel heart
SA1381372-B2-HLModel heart
SA1381382-C5-HLModel heart
SA1381392-B1-N-HLModel heart
SA1381402-B3-N-HLModel heart
SA1381412-C5-N-HLModel heart
SA1381422-B1-N-PLModel plasma
SA1381432-A1-N-PLModel plasma
SA1381442-B4-N-PLModel plasma
SA1381452-C3-N-PLModel plasma
SA1381462-C4-N-PLModel plasma
SA1381472-C1-N-PLModel plasma
SA1381482-C1-PLModel plasma
SA1381492-A4-PSModel plasma
SA1381502-C4-PSModel plasma
SA1381512-C3-PSModel plasma
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Collection:

Collection ID:CO001702
Collection Summary:Blood samples of the rats were collected to obtain plasma by centrifugation (3000r/min, 15 min) using heparin as an anticoagulant and kept at -80℃ until analysis. Hearts were collected, washed with cold saline and cut in half. One part was grinded into powder in liquid nitrogen and kept at -80℃. Heart samples were homogenized in saline on ice at the ratio of tissue to solution was 1:4 (w/v) before analysis.
Sample Type:Heart

Treatment:

Treatment ID:TR001722
Treatment Summary:Rats were divided into six rats in each group randomly as following: control rats group, MI model rats group, MI model rats pre-treated with diltiazem group (50 mg/kg, i.g.), and MI model rats pre-treated with GTSS group (200 mg/kg, i.g.). Pre-treated rats were consecutively administrated for 4 weeks. Except control rats group received saline, other rat groups received two injections of isoproterenol (85 mg/kg, s.c.) on the 27th and 28th days at an interval of 24 hours.

Sample Preparation:

Sampleprep ID:SP001715
Sampleprep Summary:100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v).

Combined analysis:

Analysis ID AN002667 AN002668
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1100 Thermo Accela 1250
Column Peeke C8 SR (150×3.0mm, 3μm) Waters Xterra MS C8 (100 x 2.1mm,3.5um)
MS Type ESI ESI
MS instrument type Triple quadrupole LTQ-FT
MS instrument name Agilent 6410 QQQ Thermo LTQ-FT
Ion Mode POSITIVE UNSPECIFIED
Units pmol/ml(PL) & pmol/mg(HL) pmol/ml(PS) & pmol/mg(HS)

Chromatography:

Chromatography ID:CH001962
Instrument Name:Agilent 1100
Column Name:Peeke C8 SR (150×3.0mm, 3μm)
Chromatography Type:Reversed phase
  
Chromatography ID:CH001963
Instrument Name:Thermo Accela 1250
Column Name:Waters Xterra MS C8 (100 x 2.1mm,3.5um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002466
Analysis ID:AN002667
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The parameters for electrospray ionization tandem MS in positive ion mode were as follows: gas temperature, 350 ℃; gas flow rate, 10 l/min; nebulizer, 30 psi; and capillary voltage, 4000V. Multiple reaction monitoring was performed using the characteristic precursor-to-product ion transitions, optimized fragmentor voltages, and collision energies.
Ion Mode:POSITIVE
  
MS ID:MS002467
Analysis ID:AN002668
Instrument Name:Thermo LTQ-FT
Instrument Type:LTQ-FT
MS Type:ESI
MS Comments:The LTQ-FT was run in full-scan mode at 100000 resolution from m/z50–1200 with MS parameters: sheath gas flow rate, 50 arb; aux gas flow rate, 20 arb; sweep gas flow rate, 3 arb; capillary temperature, 275 ℃ in both positive and negative mode. And spray voltage, 4.5kV; capillary voltage, 35.0V; tube lens, 120V were used for the positive mode. Spray voltage, -4.0kV; capillary voltage, -35.0V; tube lens, -120V were used for the negative mode.
Ion Mode:UNSPECIFIED
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