Summary of Study ST001643
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001051. The data can be accessed directly via it's Project DOI: 10.21228/M8V702 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001643 |
| Study Title | Deletion of the diabetes candidate gene Slc16a13 in mice |
| Study Summary | The metabolome of plasma and liver lysates of Slc16a13 knockout mice was analyzed. Genome-wide association studies identified SLC16A13 as novel target gene in type 2 diabetes. The SLC16A13 gene encodes for SLC16A13/MCT13, a member of the solute carrier 16 family of monocarboxylate transporters. So far, biology and physiological function of SLC16A13 is unknown. Deletion of Slc16a13 is hypothezised to affect intrahepatocellular monocarboxylate availability, that drives increased oxidative phosphorylation, while reducing hepatic lipid content, thereby attenuating hepatic insulin resistance. |
| Institute | University of California, Davis |
| Department | West Coast Metabolomics Center |
| Laboratory | Institut für Diabetesforschung und Metabolische Erkrankungen (IDM) |
| Last Name | Birkenfeld |
| First Name | Andreas |
| Address | Ottfried-Müller Str. 10 72076 Tübingen |
| Andreas.Birkenfeld@med.uni-tuebingen.de | |
| Phone | +49(0)7071 84 20257 |
| Submit Date | 2021-01-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2021-04-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001051 |
| Project DOI: | doi: 10.21228/M8V702 |
| Project Title: | Deletion of the diabetes candidate gene Slc16a13 in mice |
| Project Summary: | The metabolome of plasma and liver lysates of Slc16a13 knockout mice was analyzed. Genome-wide association studies identified SLC16A13 as novel target gene in type 2 diabetes. The SLC16A13 gene encodes for SLC16A13/MCT13, a member of the solute carrier 16 family of monocarboxylate transporters. So far, biology and physiological function of SLC16A13 is unknown. Deletion of Slc16a13 is hypothezised to affect intrahepatocellular monocarboxylate availability, that drives increased oxidative phosphorylation, while reducing hepatic lipid content, thereby attenuating hepatic insulin resistance. |
| Institute: | University of California, Davis |
| Department: | West Coast Metabolomics Center |
| Last Name: | Birkenfeld |
| First Name: | Andreas |
| Address: | Ottfried-Müller Str. 10 72076 Tübingen |
| Email: | Andreas.Birkenfeld@med.uni-tuebingen.de |
| Phone: | +49(0)7071 84 20257 |
Subject:
| Subject ID: | SU001720 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA151276 | P3_003 | KO |
| SA151277 | P2_002 | KO |
| SA151278 | P4_004 | KO |
| SA151279 | P5_005 | KO |
| SA151280 | P6_006 | KO |
| SA151281 | L1_013 | KO |
| SA151282 | P1_001 | KO |
| SA151283 | L5_017 | KO |
| SA151284 | L3_015 | KO |
| SA151285 | L2_014 | KO |
| SA151286 | L6_018 | KO |
| SA151287 | L4_016 | KO |
| SA151288 | P7_007 | WT |
| SA151289 | P12_012 | WT |
| SA151290 | P8_008 | WT |
| SA151291 | P9_009 | WT |
| SA151292 | L7_019 | WT |
| SA151293 | P11_011 | WT |
| SA151294 | P10_010 | WT |
| SA151295 | L11_023 | WT |
| SA151296 | L9_021 | WT |
| SA151297 | L12_024 | WT |
| SA151298 | L8_020 | WT |
| SA151299 | L10_022 | WT |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO001713 |
| Collection Summary: | Mice were euthanized and plasma was collected thereafter. Liver was collected and frozen in LN2 |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001733 |
| Treatment Summary: | mouse liver and plasma, wildtype and KO |
Sample Preparation:
| Sampleprep ID: | SP001726 |
| Sampleprep Summary: | Mice were euthanized and plasma was collected thereafter. Liver was collected and frozen in LN2 |
Combined analysis:
| Analysis ID | AN002688 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus IV |
| Column | Rtx-5Sil MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | UNSPECIFIED |
| Units | counts per second |
Chromatography:
| Chromatography ID: | CH001982 |
| Chromatography Summary: | A 30 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film) with additional 10 m integrated guard column is used (Restek, Bellefonte PA). 99.9999% pure Helium with built-in purifier (Airgas, Radnor PA) is set at constant flow of 1 ml/min. The oven temperature is held constant at 50°C for 1 min and then ramped at 20°C/min to 330°C at which it is held constant for 5 min. |
| Instrument Name: | Leco Pegasus IV |
| Column Name: | Rtx-5Sil MS |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002487 |
| Analysis ID: | AN002688 |
| Instrument Name: | Leco Pegasus IV TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Injector conditions: Agilent 6890 GC is equipped with a Gerstel automatic liner exchange system (ALEX) that includes a multipurpose sample (MPS2) dual rail, and a Gerstel CIS cold injection system (Gerstel, Muehlheim, Germany) with temperature program as follows: 50°C to 275°C final temperature at a rate of 12 °C/s and hold for 3 minutes. Injection volume is 0.5 μl with 10 μl/s injection speed on a splitless injector with purge time of 25 seconds. Liner (Gerstel #011711-010-00) is changed after every 10 samples, (using the Maestro1 Gerstel software vs. 1.1.4.18). Before and after each injection, the 10 μl injection syringe is washed three times with 10 μl ethyl acetate. Gas Chromatography conditions: A 30 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film) with additional 10 m integrated guard column is used (Restek, Bellefonte PA). 99.9999% pure Helium with built-in purifier (Airgas, Radnor PA) is set at constant flow of 1 ml/min. The oven temperature is held constant at 50°C for 1 min and then ramped at 20°C/min to 330°C at which it is held constant for 5 min. Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da. |
| Ion Mode: | UNSPECIFIED |
