Summary of Study ST001653
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001059. The data can be accessed directly via it's Project DOI: 10.21228/M8T69T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001653 |
Study Title | Mucosal-Associated-Invariant-T (MAIT) cells stimulated with IL-12/IL-12, anti-CD3/CD28 or both for 16 hours |
Study Summary | The untargeted metabolomics analysis was performed after metabolite extraction from vital cells. The main object of the study was to define the activation of MAIT cells on the molecular level. |
Institute | Helmholtz Centre for Environmental Research |
Last Name | Engelmann |
First Name | Beatrice |
Address | Permoserstraße 15, Leipzig, Saxony, 03418, Germany |
beatrice.engelmann@ufz.de | |
Phone | 00493412351099 |
Submit Date | 2021-01-12 |
Num Groups | 4 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2021-02-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001059 |
Project DOI: | doi: 10.21228/M8T69T |
Project Title: | Untargeted metabolomics analysis of Mucosal-Associated-Invariant-T (MAIT) cells stimulated with IL-12/IL-12, anti-CD3/CD28 or both |
Project Type: | Untargeted metabolomics |
Project Summary: | MAIT cells are unique for their ability to recognize bacterial metabolites, inducing an antigen(ag)-dependent activation, but can also be activated in an ag-independent manner but the molecular details of MAIT cell activation are not completely understood. In order to define the activation of MAIT cells on the molecular level, among other things we applied untargeted metabolomics. |
Institute: | Helmholtz Centre for Environmental Research - UFZ |
Department: | Molecular Systems Biology |
Last Name: | Engelmann |
First Name: | Beatrice |
Address: | Permoserstraße 15, Leipzig, Saxony, 03418, Germany |
Email: | beatrice.engelmann@ufz.de |
Phone: | 00493412351099 |
Subject:
Subject ID: | SU001730 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | CD161+ TCR V 7.2+ MAIT cells were obtained by positive magnetic separation after isolation of Peripheral blood mononuclear cells from male human donors. |
Species Group: | Mammals |
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA151753 | Spender60_CD3_p | a-CD3/a-CD28 activation |
SA151754 | Spender59_CD3_p | a-CD3/a-CD28 activation |
SA151755 | Spender53_CD3_p | a-CD3/a-CD28 activation |
SA151756 | Spender42_CD3_p | a-CD3/a-CD28 activation |
SA151757 | Spender49_CD3_p | a-CD3/a-CD28 activation |
SA151758 | Spender42_combi_p | combinated activation |
SA151759 | Spender60_combi_p | combinated activation |
SA151760 | Spender53_combi_p | combinated activation |
SA151761 | Spender59_combi_p | combinated activation |
SA151762 | Spender49_combi_p | combinated activation |
SA151748 | Spender49_IL12_p | IL12/IL18 activation |
SA151749 | Spender60_IL12_p | IL12/IL18 activation |
SA151750 | Spender53_IL12_p | IL12/IL18 activation |
SA151751 | Spender59_IL12_p | IL12/IL18 activation |
SA151752 | Spender42_IL12_p | IL12/IL18 activation |
SA151763 | Spender59_unstim_p | unstimulated |
SA151764 | Spender42_unstim_p | unstimulated |
SA151765 | Spender49_unstim_p | unstimulated |
SA151766 | Spender60_unstim_p | unstimulated |
SA151767 | Spender53_unstim_p | unstimulated |
Showing results 1 to 20 of 20 |
Collection:
Collection ID: | CO001723 |
Collection Summary: | Peripheral blood mononuclear cells were isolated from male human donors at age 20-50 by Ficoll-Paque™ density-gradient centrifugation. CD161+ TCR Valpha 7.2+ MAIT cells were obtained by positive magnetic separation after isolation of Peripheral blood mononuclear cells from male human donors. |
Sample Type: | Blood (whole) |
Treatment:
Treatment ID: | TR001743 |
Treatment Summary: | MAIT cells were stimulated with 50ng/ml IL-12 and 50ng/ml IL-18, 10µg/ml plate-bound anti-CD3 and 1µg/ml soluble anti-CD28 or a combination of both. |
Sample Preparation:
Sampleprep ID: | SP001736 |
Sampleprep Summary: | Cells were quenched 3 times with 1 ml ice-cold 0.9% sodium chloride. After removing the quenching solution, cells were resuspended in 100 µl ice-cold acetonitrile followed by 100 µl ice-cold Milli-Q water. After vortexing for 1 min, cells were centrifuged (14000 rpm, 4 °C, 10 min). Supernatants were transferred to new tubes. Intracellular metabolites were extracted again with 500 µl Methanol:ACN:Milli-Q water (2:3:1). After centrifugation (14000 rpm, 4 ° C, 10 min) both supernatants were combined, evaporated to dryness and stored at -80 °C until measurement. |
Combined analysis:
Analysis ID | AN002700 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 |
Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6540 QTOF |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH001992 |
Instrument Name: | Agilent 1290 |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 45 |
Flow Rate: | 0.3 ml/min |
Sample Injection: | 10 µl |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 50% acetonitrile/50% MeOH; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002498 |
Analysis ID: | AN002700 |
Instrument Name: | Agilent 6540 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Mass Hunter software was used to obtain raw data.Eluted metabolites were measured with the QTOF operated in centroid mode. Full scan data was generated with a scan range of 60-1600 m/z in positive ionization mode. Out of the survey scan the 5 most abundant precursor ions with charge state = 1 were subjected to fragmentation. The dynamic exclusion time was set at 30 s. Peak picking and database search was done using Progenesis QI software. |
Ion Mode: | POSITIVE |