Summary of Study ST001684
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001083. The data can be accessed directly via it's Project DOI: 10.21228/M8Q98J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001684 |
| Study Title | An overexpression of lipoprotein lipase leads to an alteration in the skeletal muscle metabolome in transgenic rabbits |
| Study Summary | The purpose of the current study was to characterize the skeletal muscle metabolome in the lipoprotein lipase (LPL) transgenic rabbits. The skeletal muscle metabolite profile was analyzed using capillary electrophoresis time-of flight mass spectrometry in the control rabbits and LPL transgenic rabbits (n = 9, each group). |
| Institute | Saga University |
| Last Name | Nishida |
| First Name | Yuichiro |
| Address | Department of Preventive Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501 |
| ynishida@cc.saga-u.ac.jp | |
| Phone | +81 952 34 2287 |
| Submit Date | 2020-12-03 |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001083 |
| Project DOI: | doi: 10.21228/M8Q98J |
| Project Title: | An overexpression of lipoprotein lipase leads to an alteration in the skeletal muscle metabolome in transgenic rabbits |
| Institute: | Saga University |
| Last Name: | Nishida |
| First Name: | Yuichiro |
| Email: | ynishida@cc.saga-u.ac.jp |
| Phone: | +81 952 34 2287 |
Subject:
| Subject ID: | SU001761 |
| Subject Type: | Mammal |
| Subject Species: | Oryctolagus cuniculus |
| Taxonomy ID: | 9986 |
Factors:
Subject type: Mammal; Subject species: Oryctolagus cuniculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA155392 | 2284 | Transgenic |
| SA155393 | 2285 | Transgenic |
| SA155394 | 2311 | Transgenic |
| SA155395 | 2316 | Transgenic |
| SA155396 | 2258 | Transgenic |
| SA155397 | 2312 | Transgenic |
| SA155398 | 2314 | Transgenic |
| SA155399 | 2313 | Transgenic |
| SA155400 | 2315 | Transgenic |
| SA155401 | 2262 | Wild-type |
| SA155402 | 2287 | Wild-type |
| SA155403 | 2289 | Wild-type |
| SA155404 | 2260 | Wild-type |
| SA155405 | 2317 | Wild-type |
| SA155406 | 2318 | Wild-type |
| SA155407 | 2319 | Wild-type |
| SA155408 | 2320 | Wild-type |
| SA155409 | 2322 | Wild-type |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO001754 |
| Collection Summary: | Immediately after the scarification, gastrocnemius muscle was dissected. These muscle samples were immediately frozen in liquid nitrogen, and stored at -80 degree until a metabolome analysis. |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR001774 |
| Treatment Summary: | At the end of high fat diet feeding for 16 weeks (40 weeks old), after overnight fasting, rabbits were sacrificed between 08:30 and 12:30 by overdose of thiamylal sodium. |
Sample Preparation:
| Sampleprep ID: | SP001767 |
| Sampleprep Summary: | The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement. |
Combined analysis:
| Analysis ID | AN002750 | AN002751 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Agilent Fused silica capillary (800 mm x 50 um) | Agilent Fused silica capillary (800 mm x 50 um) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative Area | Relative Area |
Chromatography:
| Chromatography ID: | CH002032 |
| Instrument Name: | Agilent CE |
| Column Name: | Agilent Fused silica capillary (800 mm x 50 um) |
| Chromatography Type: | CE |
MS:
| MS ID: | MS002547 |
| Analysis ID: | AN002750 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS002548 |
| Analysis ID: | AN002751 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
