Summary of Study ST001741

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001114. The data can be accessed directly via it's Project DOI: 10.21228/M8Q39V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001741
Study TitlePhospholipid profiling of Scd1-defective mice
Study TypeTargeted lipidomics
Study SummaryMice homozygous for the Scd1ab-2J allele have a defect Scd1 gene with an in-frame stop codon in exon 2. To identify SCD1-derived phospholipid species, we analysed PI and PC species in organs and tissues that highly express SCD1 and are considered as targets for intervention with SCD1 inhibitors, i.e., liver, skin, hind leg skeletal muscle, and white abdominal fat.
Institute
University of Innsbruck
DepartmentMichael Popp Institute
Last NameKoeberle
First NameAndreas
AddressMitterweg 24, Innsbruck, Tyrol, 6020, Austria
Emailandreas.koeberle@uibk.ac.at
Phone+43 512 507 57903
Submit Date2021-04-01
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2021-04-20
Release Version1
Andreas Koeberle Andreas Koeberle
https://dx.doi.org/10.21228/M8Q39V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001114
Project DOI:doi: 10.21228/M8Q39V
Project Title:Regulation of stress signalling by SCD1-derived phosphatidylinositols
Project Type:Targeted lipidomics
Project Summary:Cytotoxic stress activates stress-activated kinases, initiates adaptive mechanisms, including the unfolded protein response (UPR) and autophagy, and induces programmed cell death. Fatty acid unsaturation, controlled by stearoyl-CoA desaturase (SCD)1, prevents cytotoxic stress but the mechanisms are diffuse. We found that 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol) [PI(18:1/18:1)] is a SCD1-derived signaling lipid, which inhibits p38 mitogen-activated protein kinase (MAPK) activation, counteracts UPR, autophagy and apoptosis induction, and maintains cell morphology and proliferation. SCD1 expression and the cellular PI(18:1/18:1) proportion decrease during the onset of cell death, thereby activating stress signaling. This counter-regulation applies to mechanistically diverse death-inducing conditions and occurs in tissues of Scd1-defective mice.
Institute:University of Innsbruck
Department:Michael Popp Institute
Last Name:Koeberle
First Name:Andreas
Address:Mitterweg 24, Innsbruck, Tyrol, 6020, Austria
Email:andreas.koeberle@uibk.ac.at
Phone:+43 512 507 57903
Funding Source:German Research Council (GRK 1715 and KO 4589/4-1), Phospholipid Research Center Heidelberg (AKO-2019-070/2-1 and AKO-2015-037/1-1), University of Jena (DRM/2013-05 and 2.7-05), Free State of Thuringia (41-5507-2016) and Leibniz ScienceCampus InfectoOptics (SAS-2015-HKI-LWC).
Publications:PI(18:1/18:1) is a SCD1-derived lipokine that limits stress signaling. Thürmer M, Gollowitzer A, Pein H, Neukirch K, Gelmez E, Waltl L, Wielsch N, Winkler R, Löser K, Grander J, Hotze M, Harder S, Döding A, Meßner M, Troisi F, Ardelt M, Schlüter H, Pachmayr J, Gutiérrez-Gutiérrez Ó, Rudolph KL, Thedieck K, Schulze-Späte U, González-Estévez C, Kosan C, Svatoš A, Kwiatkowski M, Koeberle A. Nat Commun. 2022 May 27;13(1):2982. doi: 10.1038/s41467-022-30374-9.

Subject:

Subject ID:SU001818
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:6 weeks
Gender:Male
Animal Animal Supplier:Jackson Laboratory

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Tissue
SA163124200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#51 - 200916_SCD_1_KO_F7_KO_n1Scd1ab-2J Fat
SA163125200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#54 - 200916_SCD_1_KO_F10_KO_n4Scd1ab-2J Fat
SA163126200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#52 - 200916_SCD_1_KO_F8_KO_n2Scd1ab-2J Fat
SA163127200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#51 - 200916_SCD_1_KO_F7_KO_n1Scd1ab-2J Fat
SA163128200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#53 - 200916_SCD_1_KO_F9_KO_n3Scd1ab-2J Fat
SA163129200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#55 - 200916_SCD_1_KO_F11_KO_n5Scd1ab-2J Fat
SA163130200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#52 - 200916_SCD_1_KO_F8_KO_n2Scd1ab-2J Fat
SA163131200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#55 - 200916_SCD_1_KO_F11_KO_n5Scd1ab-2J Fat
SA163132200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#54 - 200916_SCD_1_KO_F10_KO_n4Scd1ab-2J Fat
SA163133200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#53 - 200916_SCD_1_KO_F9_KO_n3Scd1ab-2J Fat
SA163134200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#41 - 200916_SCD_1_KO_L11_KO_n5Scd1ab-2J Liver
SA163135200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#40 - 200916_SCD_1_KO_L10_KO_n4Scd1ab-2J Liver
SA163136200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#37 - 200916_SCD_1_KO_L7_KO_n1Scd1ab-2J Liver
SA163137200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#38 - 200916_SCD_1_KO_L8_KO_n2Scd1ab-2J Liver
SA163138200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#39 - 200916_SCD_1_KO_L9_KO_n3Scd1ab-2J Liver
SA163139200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#41 - 200916_SCD_1_KO_L11_KO_n5Scd1ab-2J Liver
SA163140200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#40 - 200916_SCD_1_KO_L10_KO_n4Scd1ab-2J Liver
SA163141200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#37 - 200916_SCD_1_KO_L7_KO_n1Scd1ab-2J Liver
SA163142200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#39 - 200916_SCD_1_KO_L9_KO_n3Scd1ab-2J Liver
SA163143200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#38 - 200916_SCD_1_KO_L8_KO_n2Scd1ab-2J Liver
SA163144200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#23 - 200916_SCD_1_KO_M7_KO_n1Scd1ab-2J Skeletal muscle
SA163145200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#24 - 200916_SCD_1_KO_M8_KO_n2Scd1ab-2J Skeletal muscle
SA163146200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#26 - 200916_SCD_1_KO_M10_KO_n4Scd1ab-2J Skeletal muscle
SA163147200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#25 - 200916_SCD_1_KO_M9_KO_n3Scd1ab-2J Skeletal muscle
SA163148200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#24 - 200916_SCD_1_KO_M8_KO_n2Scd1ab-2J Skeletal muscle
SA163149200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#23 - 200916_SCD_1_KO_M7_KO_n1Scd1ab-2J Skeletal muscle
SA163150200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#25 - 200916_SCD_1_KO_M9_KO_n3Scd1ab-2J Skeletal muscle
SA163151200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#27 - 200916_SCD_1_KO_M11_KO_n5Scd1ab-2J Skeletal muscle
SA163152200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#26 - 200916_SCD_1_KO_M10_KO_n4Scd1ab-2J Skeletal muscle
SA163153200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#27 - 200916_SCD_1_KO_M11_KO_n5Scd1ab-2J Skeletal muscle
SA163154200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#13 - 200916_SCD_1_KO_S11_KO_n5_repeatScd1ab-2J Skin
SA163155200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#9 - 200916_SCD_1_KO_S7_KO_n1Scd1ab-2J Skin
SA163156200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#10 - 200916_SCD_1_KO_S8_KO_n2Scd1ab-2J Skin
SA163157200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#11 - 200916_SCD_1_KO_S9_KO_n3Scd1ab-2J Skin
SA163158200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#12 - 200916_SCD_1_KO_S10_KO_n4Scd1ab-2J Skin
SA163159200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#10 - 200916_SCD_1_KO_S8_KO_n2Scd1ab-2J Skin
SA163160200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#12 - 200916_SCD_1_KO_S10_KO_n4Scd1ab-2J Skin
SA163161200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#58 - 200916_SCD_1_KO_S11_KO_n5_repeatScd1ab-2J Skin
SA163162200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#11 - 200916_SCD_1_KO_S9_KO_n3Scd1ab-2J Skin
SA163163200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#9 - 200916_SCD_1_KO_S7_KO_n1Scd1ab-2J Skin
SA163164200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#48 - 200916_SCD_1_KO_F5_WT_n5WT Fat
SA163165200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#47 - 200916_SCD_1_KO_F4_WT_n4WT Fat
SA163166200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#45 - 200916_SCD_1_KO_F2_WT_n2WT Fat
SA163167200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#46 - 200916_SCD_1_KO_F3_WT_n3WT Fat
SA163168200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#49 - 200916_SCD_1_KO_F6_WT_n6WT Fat
SA163169200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#44 - 200916_SCD_1_KO_F1_WT_n1WT Fat
SA163170200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#49 - 200916_SCD_1_KO_F6_WT_n6WT Fat
SA163171200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#46 - 200916_SCD_1_KO_F3_WT_n3WT Fat
SA163172200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#45 - 200916_SCD_1_KO_F2_WT_n2WT Fat
SA163173200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#47 - 200916_SCD_1_KO_F4_WT_n4WT Fat
SA163174200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#48 - 200916_SCD_1_KO_F5_WT_n5WT Fat
SA163175200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#44 - 200916_SCD_1_KO_F1_WT_n1WT Fat
SA163176200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#30 - 200916_SCD_1_KO_L1_WT_n1WT Liver
SA163177200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#30 - 200916_SCD_1_KO_L1_WT_n1WT Liver
SA163178200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#31 - 200916_SCD_1_KO_L2_WT_n2WT Liver
SA163179200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#31 - 200916_SCD_1_KO_L2_WT_n2WT Liver
SA163180200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#35 - 200916_SCD_1_KO_L6_WT_n6WT Liver
SA163181200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#32 - 200916_SCD_1_KO_L3_WT_n3WT Liver
SA163182200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#34 - 200916_SCD_1_KO_L5_WT_n5WT Liver
SA163183200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#33 - 200916_SCD_1_KO_L4_WT_n4WT Liver
SA163184200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#32 - 200916_SCD_1_KO_L3_WT_n3WT Liver
SA163185200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#33 - 200916_SCD_1_KO_L4_WT_n4WT Liver
SA163186200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#34 - 200916_SCD_1_KO_L5_WT_n5WT Liver
SA163187200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#35 - 200916_SCD_1_KO_L6_WT_n6WT Liver
SA163188200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#19 - 200916_SCD_1_KO_M4_WT_n4WT Skeletal muscle
SA163189200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#20 - 200916_SCD_1_KO_M5_WT_n5WT Skeletal muscle
SA163190200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#18 - 200916_SCD_1_KO_M2_WT_n3WT Skeletal muscle
SA163191200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#16 - 200916_SCD_1_KO_M1_WT_n1WT Skeletal muscle
SA163192200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#17 - 200916_SCD_1_KO_M2_WT_n2WT Skeletal muscle
SA163193200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#16 - 200916_SCD_1_KO_M1_WT_n1WT Skeletal muscle
SA163194200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#21 - 200916_SCD_1_KO_M6_WT_n6WT Skeletal muscle
SA163195200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#20 - 200916_SCD_1_KO_M5_WT_n5WT Skeletal muscle
SA163196200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#19 - 200916_SCD_1_KO_M4_WT_n4WT Skeletal muscle
SA163197200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#18 - 200916_SCD_1_KO_M2_WT_n3WT Skeletal muscle
SA163198200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#17 - 200916_SCD_1_KO_M2_WT_n2WT Skeletal muscle
SA163199200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#21 - 200916_SCD_1_KO_M6_WT_n6WT Skeletal muscle
SA163200200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#2 - 200916_SCD_1_KO_S1_WT_n1WT Skin
SA163201200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#6 - 200916_SCD_1_KO_S2_WT_n5WT Skin
SA163202200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#5 - 200916_SCD_1_KO_S2_WT_n4WT Skin
SA163203200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#4 - 200916_SCD_1_KO_S2_WT_n3WT Skin
SA163204200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#3 - 200916_SCD_1_KO_S2_WT_n2WT Skin
SA163205200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#7 - 200916_SCD_1_KO_S2_WT_n6WT Skin
SA163206200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#2 - 200916_SCD_1_KO_S1_WT_n1WT Skin
SA163207200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#6 - 200916_SCD_1_KO_S2_WT_n5WT Skin
SA163208200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#5 - 200916_SCD_1_KO_S2_WT_n4WT Skin
SA163209200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#4 - 200916_SCD_1_KO_S2_WT_n3WT Skin
SA163210200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#3 - 200916_SCD_1_KO_S2_WT_n2WT Skin
SA163211200916_SCD_1_KO_in_vivo_PC_dil_1_20_SMLF_#7 - 200916_SCD_1_KO_S2_WT_n6WT Skin
Showing results 1 to 88 of 88

Collection:

Collection ID:CO001811
Collection Summary:Mouse tissues were homogenized in PBS pH 7.4 for lipid extraction and UPLC-MS/MS analysis
Sample Type:Tissue
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001831
Treatment Summary:Tissues from 6-week old male C57BL/6J mice (wildtype or homozygous for the Scd1ab-2J allele) were purchased from Jackson Laboratory.

Sample Preparation:

Sampleprep ID:SP001824
Sampleprep Summary:Phospholipids were extracted from tissue homogenates by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS.
Extract Storage:-20℃

Combined analysis:

Analysis ID AN002834
Analysis type MS
Chromatography type Reversed phase
Chromatography system ExionLC
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 6500+ Qtrap
Ion Mode NEGATIVE
Units relative intensities

Chromatography:

Chromatography ID:CH002097
Chromatography Summary:Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an ExionLC UHPLC.
Instrument Name:ExionLC
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002627
Analysis ID:AN002834
Instrument Name:ABI Sciex API 6500+ Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.7 (Sciex).
Ion Mode:NEGATIVE
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