Summary of Study ST001742

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001115. The data can be accessed directly via it's Project DOI: 10.21228/M8KD8B This work is supported by NIH grant, U2C- DK119886.

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Study IDST001742
Study TitleMetabolomic Analysis of Canine Diabetes (part-I)
Study TypeSingle time point blood collection
Study SummaryBlood was collected from diabetic dogs and healthy control dogs. Diabetic dogs and control dogs were breed matched where possible. Timing of blood collection after a meal was matched as best as posible. Serum was frozen at -80C.
Institute
University of Florida
Last NameO'Kell
First NameAllison
Address2015 SW 16th Ave
Emailaokell@ufl.edu
Phone3522944471
Submit Date2021-03-31
Num Groups2
Total Subjects80
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-03-31
Release Version1
Allison O'Kell Allison O'Kell
https://dx.doi.org/10.21228/M8KD8B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001115
Project DOI:doi: 10.21228/M8KD8B
Project Title:Utility of canine diabetes as a model for human type 1 diabetes (part-I)
Project Summary:We are examining the etiopathogenesis of canine diabetes to determine the relevance of these animals as a naturally occurring model of human type 1 diabetes. We will undertake phenotypic, metabolic, and immunologic investigations in diabetic dogs to better characterize their diabetes and attempt to identify potential biomarkers of a pre-diabetic state.
Institute:University of Florida
Department:Diabetes Institute (Wasserfall/Atkinson)/College of Veterinary Medicine (O'Kell)
Last Name:O'Kell
First Name:Allison
Address:2015 SW 16th Ave
Email:aokell@ufl.edu
Phone:352-294-4471

Subject:

Subject ID:SU001819
Subject Type:Mammal
Subject Species:Canis lupus familiaris
Taxonomy ID:9615
Age Or Age Range:Adult
Weight Or Weight Range:3-60 kg

Factors:

Subject type: Mammal; Subject species: Canis lupus familiaris (Factor headings shown in green)

mb_sample_id local_sample_id Group Fasted vs Fed
SA163212321588Diabetic Fasted
SA163213325419Diabetic Fasted
SA163214313074Diabetic Fasted
SA163215353791Diabetic Fasted
SA163216349937Diabetic Fasted
SA163217346336Diabetic Fasted
SA163218354675Diabetic Fasted
SA163219349833Diabetic Fasted
SA163220355594Diabetic Fasted
SA163221329649Diabetic Fasted
SA163222213269Diabetic Fasted
SA163223213267Diabetic Fasted
SA163224325942Diabetic Fasted
SA163225325103Diabetic Fasted
SA163226313390Diabetic Fasted
SA163227325643Diabetic Fed
SA163228269031Diabetic Fed
SA163229279436Diabetic Fed
SA163230319373Diabetic Fed
SA163231327679Diabetic Fed
SA163232242045Diabetic Fed
SA163233348332Diabetic Fed
SA163234333987Diabetic Fed
SA163235323294Diabetic Fed
SA163236308098Diabetic Fed
SA163237309443Diabetic Fed
SA163238216422Diabetic Fed
SA163239292894Healthy Fasted
SA163240243765Healthy Fasted
SA163241360399Healthy Fasted
SA163242359130Healthy Fasted
SA163243309999Healthy Fasted
SA163244325866Healthy Fasted
SA163245298006Healthy Fasted
SA163246301154Healthy Fasted
SA163247290374Healthy Fasted
SA163248355837Healthy Fasted
SA163249344481Healthy Fasted
SA163250255293Healthy Fasted
SA163251295029Healthy Fasted
SA163252228415Healthy Fasted
SA163253288261Healthy Fasted
SA163254232368Healthy Fed
SA163255328335Healthy Fed
SA163256295509Healthy Fed
SA163257280189Healthy Fed
SA163258274821Healthy Fed
SA163259287971Healthy Fed
SA163260277908Healthy Fed
SA163261325932Healthy Fed
SA163262223169Healthy Fed
SA163263296735Healthy Fed
SA163264290195Healthy Fed
SA163265326077Healthy Fed
Showing results 1 to 54 of 54

Collection:

Collection ID:CO001812
Collection Summary:Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7.5 minutes between 25-35 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C.
Collection Protocol Filename:Protein_Precipitation_Sample_Prep.pdf
Sample Type:Blood (serum)
Collection Method:Peripheral venipuncture
Collection Location:Jugular, saphenous, or cephalic vein
Collection Frequency:Once
Volumeoramount Collected:Variable
Storage Conditions:-80℃
Collection Vials:Red top with clot activator
Storage Vials:Cryovials (polypropylene); Microcentrifuge tubes (polypropylene) for second round
Collection Tube Temp:Room temperature

Treatment:

Treatment ID:TR001832
Treatment Summary:None for research study. Variable medications may have been received by diabetic dogs. Healthy dogs are only allowed to receive flea/tick/heartworm preventatives.

Sample Preparation:

Sampleprep ID:SP001825
Sampleprep Summary:TRP Quant_Protein Precipitation Sample Prep.docx
Sampleprep Protocol Filename:Protein_Precipitation_Sample_Prep.pdf

Combined analysis:

Analysis ID AN002835
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units ng/mL

Chromatography:

Chromatography ID:CH002098
Chromatography Summary:Protein Precipitation Sample Prep.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002628
Analysis ID:AN002835
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Protein Precipitation Sample Prep.pdf
Ion Mode:POSITIVE
Analysis Protocol File:Protein_Precipitation_Sample_Prep.pdf
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