Summary of Study ST001749

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001121. The data can be accessed directly via it's Project DOI: 10.21228/M8SX23 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001749
Study TitleREACH Metabolomics Study
Study SummaryIncreasing evidence indicates that physical activity and exercise training may delay or prevent the onset of Alzheimer’s disease (AD). However, systemic biomarkers that can measure exercise effects on brain function and that link to relevant metabolic responses are lacking. This study utilized blood samples of 23 asymptomatic late middle-aged adults with familial and genetic risk for AD who underwent 26 weeks of supervised treadmill training. Metabolomic profiles were evaluated using MS.
Institute
University of Wisconsin-Madison
DepartmentMedicine
LaboratoryWisconsin Alzheimer's Disease Research Center
Last NameGaitán
First NameJulian
Address600 Highland Ave. J5/1M CSC MC2420, Madison, WI 53792
Emailjgaitan@medicine.wisc.edu
Phone608-263-2245
Submit Date2021-04-15
Num Groups2
Total Subjects23
Num Males12
Num Females11
Analysis Type DetailLC-MS
Release Date2021-05-11
Release Version1
Julian Gaitán Julian Gaitán
https://dx.doi.org/10.21228/M8SX23
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001121
Project DOI:doi: 10.21228/M8SX23
Project Title:REACH Metabolomics Study
Project Summary:Increasing evidence indicates that physical activity and exercise training may delay or prevent the onset of Alzheimer’s disease (AD). However, systemic biomarkers that can measure exercise effects on brain function and that link to relevant metabolic responses are lacking. This study utilized blood samples of 23 asymptomatic late middle-aged adults with familial and genetic risk for AD who underwent 26 weeks of supervised treadmill training. Metabolomic profiles were evaluated using MS.
Institute:University of Wisconsin - Madison
Department:Medicine
Laboratory:Wisconsin Alzheimer's Disease Research Center
Last Name:Gaitán
First Name:Julian M
Address:600 Highland Ave. J5/1M CSC MC2420, Madison, WI 53792
Email:jgaitan@medicine.wisc.edu
Phone:608-263-2245

Subject:

Subject ID:SU001826
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:55 - 73
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Timepoint
SA163785UWIM-11196Enhanced Physical Activity Post
SA163786UWIM-11195Enhanced Physical Activity Post
SA163787UWIM-11193Enhanced Physical Activity Post
SA163788UWIM-11200Enhanced Physical Activity Post
SA163789UWIM-11194Enhanced Physical Activity Post
SA163790UWIM-11201Enhanced Physical Activity Post
SA163791UWIM-11206Enhanced Physical Activity Post
SA163792UWIM-11204Enhanced Physical Activity Post
SA163793UWIM-11202Enhanced Physical Activity Post
SA163794UWIM-11190Enhanced Physical Activity Post
SA163795UWIM-11187Enhanced Physical Activity Post
SA163796UWIM-11171Enhanced Physical Activity Pre
SA163797UWIM-11164Enhanced Physical Activity Pre
SA163798UWIM-11172Enhanced Physical Activity Pre
SA163799UWIM-11173Enhanced Physical Activity Pre
SA163800UWIM-11167Enhanced Physical Activity Pre
SA163801UWIM-11170Enhanced Physical Activity Pre
SA163802UWIM-11183Enhanced Physical Activity Pre
SA163803UWIM-11178Enhanced Physical Activity Pre
SA163804UWIM-11177Enhanced Physical Activity Pre
SA163805UWIM-11179Enhanced Physical Activity Pre
SA163806UWIM-11181Enhanced Physical Activity Pre
SA163807UWIM-11199Usual Physical Activity Post
SA163808UWIM-11198Usual Physical Activity Post
SA163809UWIM-11197Usual Physical Activity Post
SA163810UWIM-11203Usual Physical Activity Post
SA163811UWIM-11192Usual Physical Activity Post
SA163812UWIM-11188Usual Physical Activity Post
SA163813UWIM-11191Usual Physical Activity Post
SA163814UWIM-11207Usual Physical Activity Post
SA163815UWIM-11209Usual Physical Activity Post
SA163816UWIM-11189Usual Physical Activity Post
SA163817UWIM-11205Usual Physical Activity Post
SA163818UWIM-11208Usual Physical Activity Post
SA163819UWIM-11165Usual Physical Activity Pre
SA163820UWIM-11166Usual Physical Activity Pre
SA163821UWIM-11168Usual Physical Activity Pre
SA163822UWIM-11174Usual Physical Activity Pre
SA163823UWIM-11184Usual Physical Activity Pre
SA163824UWIM-11182Usual Physical Activity Pre
SA163825UWIM-11180Usual Physical Activity Pre
SA163826UWIM-11185Usual Physical Activity Pre
SA163827UWIM-11186Usual Physical Activity Pre
SA163828UWIM-11176Usual Physical Activity Pre
SA163829UWIM-11175Usual Physical Activity Pre
SA163830UWIM-11169Usual Physical Activity Pre
Showing results 1 to 46 of 46

Collection:

Collection ID:CO001819
Collection Summary:Fasting blood samples were collected into a 10mL EDTA vacutainer which was gently inverted 10–12 times. Within one hour of collection, EDTA tubes were centrifuged at 3000rpm for 15 minutes at room temperature. Cell-free plasma was aliquoted into cryovials and stored at –80° C and was not thawed until assays were run.
Sample Type:Blood (plasma)
Collection Method:Fasting venipuncture
Storage Conditions:-80℃
Collection Vials:EDTA Vacutainer
Storage Vials:Cryovial

Treatment:

Treatment ID:TR001839
Treatment Summary:Participants were randomized after baseline assessment to either Usual Physical Activity (UPA) or Enhanced Physical Activity (EPA) using 1:1 block randomization accounting for age and sex. UPA group was given educational material about maintaining a healthy and active lifestyle. The EPA group engaged in a supervised, progressive, moderate-to-vigorous intensity aerobic exercise training program three times per week for 50 minutes per session from week seven through 26.

Sample Preparation:

Sampleprep ID:SP001832
Sampleprep Summary:Samples were prepared using the MicroLab STAR system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.

Combined analysis:

Analysis ID AN002848 AN002849 AN002850 AN002851
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase HILIC
Chromatography system Waters Acquity Waters Acquity Waters Acquity Waters Acquity
Column Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE POSITIVE NEGATIVE NEGATIVE
Units Raw area counts Raw area counts Raw area counts Raw area counts

Chromatography:

Chromatography ID:CH002109
Chromatography Summary:Two fractions were analyzed using positive ion mode electrospray ionization optimized for hydrophilic compounds. One fraction was analyzed using negative ion mode electrospray ionization.
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH002110
Chromatography Summary:One fraction was analyzed via negative ion mode electrospray ionization from Hydrophilic-Interaction Chromatography (HILIC) column.
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS002641
Analysis ID:AN002848
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:POSITIVE
  
MS ID:MS002642
Analysis ID:AN002849
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:POSITIVE
  
MS ID:MS002643
Analysis ID:AN002850
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:NEGATIVE
  
MS ID:MS002644
Analysis ID:AN002851
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:NEGATIVE
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