Summary of Study ST001780

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001133. The data can be accessed directly via it's Project DOI: 10.21228/M87X24 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001780
Study TitleComparative analysis of metabolomic profiles in cerebrospinal fluid before and after endurance exercise
Study SummaryThis study compared the metabolomic differences in the cerebrospinal fluid of young adult volunteers between before (Pre), and 60 minutes after the 90-min run (Post).
Institute
University of California, San Diego School of Medicine
Last NameNaviaux
First NameRobert
Address214 Dickinson St., Room C-107, San Diego, CA, 92103, USA
Emailrnaviaux@health.ucsd.edu
Phone619-543-2904
Submit Date2021-05-03
Num Groups2
Total Subjects19
Num Males13
Num Females6
Analysis Type DetailLC-MS
Release Date2021-11-25
Release Version1
Robert Naviaux Robert Naviaux
https://dx.doi.org/10.21228/M87X24
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001133
Project DOI:doi: 10.21228/M87X24
Project Title:Metabolomic changes of cerebrospinal fluid in response to endurance exercise
Project Summary:Metabolomics has emerged as a powerful new tool in precision medicine for gaining mechanistic insight into health and disease. The objective of this project was to investigate the metabolomic changes in cerebrospinal fluid (CSF) in response to endurance exercise. Young adult volunteers were enrolled, and the participants completed a monitored outdoor 90-minute run. CSF samples were collected during rest (Pre), and 60 minutes after the run (Post). Broad-spectrum targeted metabolomics was conducted using the liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This study provided insights into the neuroprotective and mental health benefits of endurance exercise.
Institute:University of California, San Diego School of Medicine
Last Name:Naviaux
First Name:Robert
Address:214 Dickinson St., Room C-107, San Diego, CA, 92103, USA
Email:rnaviaux@health.ucsd.edu
Phone:619-543-2904

Subject:

Subject ID:SU001857
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:25(IQR 22-31)
Gender:Male and female
Human Race:European
Human Medications:None
Human Prescription Otc:None
Human Smoking Status:None
Human Alcohol Drug Use:None

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA165061LP16_post_CSF_MPost
SA165062LP18_post_CSF_MPost
SA165063LP21_post_CSF_MPost
SA165064LP14_post_CSF_MPost
SA165065LP23_post_CSF_FPost
SA165066LP19_post_CSF_FPost
SA165067LP20_post_CSF_FPost
SA165068LP22_post_CSF_MPost
SA165069LP30_post_CSF_FPost
SA165070LP24_post_CSF_MPost
SA165071LP29_post_CSF_MPost
SA165072LP31_post_CSF_MPost
SA165073LP32_post_CSF_MPost
SA165074LP28_post_CSF_MPost
SA165075LP27_post_CSF_MPost
SA165076LP25_post_CSF_MPost
SA165077LP26_post_CSF_MPost
SA165078LP15_post_CSF_FPost
SA165079LP11_post_CSF_FPost
SA165080LP14_pre_CSF_MPre
SA165081LP16_pre_CSF_MPre
SA165082LP18_pre_CSF_MPre
SA165083LP30_pre_CSF_FPre
SA165084LP23_pre_CSF_FPre
SA165085LP15_pre_CSF_FPre
SA165086LP19_pre_CSF_FPre
SA165087LP20_pre_CSF_FPre
SA165088LP21_pre_CSF_MPre
SA165089LP22_pre_CSF_MPre
SA165090LP29_pre_CSF_MPre
SA165091LP31_pre_CSF_MPre
SA165092LP32_pre_CSF_MPre
SA165093LP28_pre_CSF_MPre
SA165094LP27_pre_CSF_MPre
SA165095LP24_pre_CSF_MPre
SA165096LP25_pre_CSF_MPre
SA165097LP26_pre_CSF_MPre
SA165098LP11_pre_CSF_FPre
Showing results 1 to 38 of 38

Collection:

Collection ID:CO001850
Collection Summary:Measurements and samples were collected on two days separated by 4 weeks. Samples were drawn at the same time on the two experimental days to control for circadian rhythms. A baseline cerebrospinal fluid (CSF) sample (Pre) was collected. Participants were given a banana (~100g) then completed a 90-minute outdoor run at local time 09:00 to 10:30 am. After 60 minutes of recovery (1130 am local time), a post-run CSF sample was collected (Post).
Sample Type:CSF
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001870
Treatment Summary:Participants were given a banana (~100 g) then completed a 90-minute outdoor run at local time 09:00 to 10:30 am, maintaining an effort of 75% -80% of their heart rate. A baseline cerebrospinal fluid sample (CSF) (Pre) was collected before the run. After 60 minutes of recovery (1130 am local time), a post-run CSF sample was collected (Post).

Sample Preparation:

Sampleprep ID:SP001863
Sampleprep Summary:The cerebrospinal fluid samples (90 µl) were mixed with 5 µl of commercial internal standards and 5 µl of custom-synthesized 13C labeled standards and then incubated for 10 min at room temperature to permit small molecules and vitamins in the internal standards to associate with binding proteins. The metabolites were extracted with 400 µl of cold (-20 °C) methanol: acetonitrile (50:50, v/v), yielding a final concentration of methanol: acetonitrile: water (40:40:20, v/v/v). The samples were then vortexed vigorously, incubated on crushed ice for 10 min, and then centrifuged at 16,000 g for 10 min at 4 °C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80 °C prior to LC-MS/MS.
Processing Storage Conditions:On ice
Extract Cleanup:None
Extract Storage:-80℃
Sample Resuspension:None
Sample Derivatization:None

Combined analysis:

Analysis ID AN002890 AN002891
Analysis type MS MS
Chromatography type HILIC Reversed phase
Chromatography system Shimadzu 20AD Shimadzu 20AD
Column Shodex NH2P-40 2E (250 x 2mm,4um) Raptor Biphenyl (150 × 2.1 mm,2.7um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED UNSPECIFIED
Units peak area peak area

Chromatography:

Chromatography ID:CH002142
Chromatography Summary:Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in HILIC mode using a polymer-based NH2 column (250 × 2 mm, 4 µm) (Asahipak NH2P-40 2E, Shodex, Japan). The optimal LC conditions were as follows: Mobile phase A: 95% H2O with 20 mM (NH4)2CO3 and 5% ACN, pH 9.8. Mobile phase B: 100% ACN. The gradient was: 0 - 3.5 min 95% B, 3.6 - 8 min 85% B, 8.1 - 13 min 75% B, 14 - 30 min 0% B, 31 - 41 min 95% B, 41.1 min stop. The flow rate was 200 µL/min, and the column temperature was held at 25 ºC.
Instrument Name:Shimadzu 20AD
Column Name:Shodex NH2P-40 2E (250 x 2mm,4um)
Column Pressure:900 psi - 2400 psi
Column Temperature:25
Flow Gradient:Constant
Flow Rate:200 µL/min
Injection Temperature:4
Sample Injection:10 uL
Solvent A:95% water/5% acetonitrile; 20 mM ammonium acetate, pH 9.8
Solvent B:100% acetonitrile
Analytical Time:45 min
Oven Temperature:25
Sample Loop Size:10
Sample Syringe Size:100
Chromatography Type:HILIC
  
Chromatography ID:CH002143
Chromatography Summary:Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in the RP model using a Raptor Biphenyl column (150 × 2.1 mm, 2.7 µm) (Restek, PA). The LC conditions were as follows: Mobile phase A: 90% H2O with 0.1% formic acid and 10% MEOH, pH 4.0. Mobile phase B: MEOH-IPA (50:50, v/v) with 0.1% formic acid. The gradient was: 0 - 2 min 10% B, 2.1 - 4 min 40% B, 4 -12 min, linear ramping up to 100% B, 12 – 18 min 100% B, 19 - 24 min 10% B, 24.1 min stop. The flow rate was 250 µL/min, and the column temperature was controlled at 40 ºC.
Instrument Name:Shimadzu 20AD
Column Name:Raptor Biphenyl (150 × 2.1 mm,2.7um)
Column Pressure:2800 psi - 4200 psi
Column Temperature:40
Flow Gradient:Constant
Flow Rate:250 µL/min
Injection Temperature:4
Sample Injection:10 uL
Solvent A:90% water/10% methanol; 0.1% formic acid, pH 4.0
Solvent B:50% methanol/50% isopropanol; 0.1% formic acid
Analytical Time:25 min
Oven Temperature:40
Sample Loop Size:10
Sample Syringe Size:100
Chromatography Type:Reversed phase

MS:

MS ID:MS002683
Analysis ID:AN002890
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The MS/MS detection was performed using electrospray ionization (ESI) on both positive and negative modes. The MS/MS detection was performed using electrospray ionization (ESI) on both positive and negative modes. Advanced scheduled multiple reaction monitoring (MRM) with dynamic windowing and fast polarity switch were used. The ESI source conditions were set as follows: electrospray voltage of -4500 V for negative mode and 5500 V for positive mode, source temperature of 500 °C, curtain gas of 30, ion source gas 1, and gas 2 of 35 psi, respectively.
Ion Mode:UNSPECIFIED
Collision Energy:Compound-dependent
Collision Gas:Medium
Ion Source Temperature:500
Ion Spray Voltage:-4500 V for negative and 5500 V for positive
Ionization:ESI
Mass Accuracy:0.1 Da
Source Temperature:500
  
MS ID:MS002684
Analysis ID:AN002891
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:The MS/MS detection was performed using electrospray ionization (ESI) on both positive and negative modes. The MS/MS detection was performed using electrospray ionization (ESI) on both positive and negative modes. Advanced scheduled multiple reaction monitoring (MRM) with dynamic windowing and fast polarity switch were used. The ESI source conditions were set as follows: electrospray voltage of -4500 V for negative mode and 5500 V for positive mode, source temperature of 500 °C, curtain gas of 30, ion source gas 1, and gas 2 of 35 psi, respectively.
Ion Mode:UNSPECIFIED
Collision Energy:Compound-dependent
Collision Gas:Medium
Ion Source Temperature:500
Ion Spray Voltage:-4500 V for negative and 5500 V for positive
Ionization:ESI
Mass Accuracy:0.1 Da
Source Temperature:500
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