Summary of Study ST001794

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001138. The data can be accessed directly via it's Project DOI: 10.21228/M8M68K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001794
Study TitleMetabolomics Analysis of Time-Series Gastrointestinal Lumen Samples
Study SummarySamples were retrieved from a human small intestine samples over 8 hours. Metabolomics analysis followed resulting in many annotated metabolites. Intensity profiles gives insight into gastrointestinal functions.
Institute
University of California, Davis
Last NameFolz
First NameJacob
Address451 Health Science Drive
Emailjfolz@ucdavis.edu
Phone7155636311
Submit Date2021-05-17
Total Subjects1
Num Males1
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-06-01
Release Version1
Jacob Folz Jacob Folz
https://dx.doi.org/10.21228/M8M68K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001138
Project DOI:doi: 10.21228/M8M68K
Project Title:Metabolomics Analysis of Time-Series Gastrointestinal Lumen Samples
Project Type:Exploratory
Project Summary:Samples were retrieved from a human small intestine samples over 8 hours. Metabolomics analysis followed resulting in many annotated metabolites. Intensity profiles gives insight into gastrointestinal functions.
Institute:University of California, Davis
Last Name:Folz
First Name:Jacob
Address:451 Health Science Drive
Email:jfolz@ucdavis.edu
Phone:7155636311

Subject:

Subject ID:SU001871
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA167125QC_01QC
SA167126QC_03QC
SA167127QC_02QC
SA167128QC_04QC
SA167129Sa_01Time point 1
SA167130Sa_10Time point 10
SA167131Sa_11Time point 11
SA167132Sa_12Time point 12
SA167133Sa_13Time point 13
SA167134Sa_14Time point 14
SA167135Sa_15Time point 15
SA167136Sa_16Time point 16
SA167137Sa_17Time point 17
SA167138Sa_18Time point 18
SA167139Sa_19Time point 19
SA167140Sa_02Time point 2
SA167141Sa_20Time point 20
SA167142Sa_03Time point 3
SA167143Sa_04Time point 4
SA167144Sa_05Time point 5
SA167145Sa_06Time point 6
SA167146Sa_07Time point 7
SA167147Sa_08Time point 8
SA167148Sa_09Time point 9
Showing results 1 to 24 of 24

Collection:

Collection ID:CO001864
Collection Summary:Samples were retrieved from the lumen of the jejunum of a human subject over 8 hours collected at approximately 30 minute intervals.
Sample Type:Jejunum

Treatment:

Treatment ID:TR001884
Treatment Summary:Diet and acetaminophen consumption were recorded throughout the collection period.

Sample Preparation:

Sampleprep ID:SP001877
Sampleprep Summary:20 uL of jejunum supernatant was extracted using a biphasic liquid liquid extraction for HILIC and CSH LC-MS/MS analysis as in doi: 10.3390/metabo9050101

Chromatography:

Chromatography ID:CH002157
Chromatography Summary:HILIC
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:The gradient started at 100% B, was held at 100% B from 0-2 minutes, decreased to 70% B between 2-7.7 minutes, decreased to 40% between 7.7 and 9.5 minutes, was held at 40% B from 9.5-12.75 minutes, returned to 100% B between 12.75-12.85 minutes, and was held at 100% B from 12.85-17 minutes.
Flow Rate:0.4 ml/min
Sample Injection:3 uL
Solvent A:100% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:95% acetonitrile/5% water; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:HILIC
  
Chromatography ID:CH002158
Chromatography Summary:CSH
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65
Flow Gradient:The LC gradient started at 15% B, increased to 30% B from 0-2 minutes, increased to 48% B from 2-2.5 minutes, increased to 82% B from 2.5-11 minutes, increased to 99% B from 11-11.5 minutes, held at 99% B from 11.5-12 minutes, returned to 15% B from 12-12.1 minutes and held at 15% B from 12.1-15 minutes.
Flow Rate:0.6 ml/min
Sample Injection:4 uL
Solvent A:40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate(+ mode); 40% water/60% acetonitrile; 10 mM ammonium acetate(- mode)
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate for positive mode ionization, and 10mM ammonium acetate for negative mode ionization.
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN002911
Laboratory Name:Fiehn Lab
Analysis Type:MS
Operator Name:Jacob Folz
Chromatography ID:CH002157
Num Factors:21
Num Metabolites:339
Rt Units:Minutes
Units:Peak Height Intensity
  
Analysis ID:AN002912
Laboratory Name:Fiehn Lab
Analysis Type:MS
Operator Name:Jacob Folz
Chromatography ID:CH002157
Num Factors:21
Num Metabolites:242
Rt Units:Minutes
Units:Peak Height Intensity
  
Analysis ID:AN002913
Laboratory Name:Fiehn Lab
Analysis Type:MS
Operator Name:Jacob Folz
Chromatography ID:CH002158
Num Factors:21
Num Metabolites:204
Rt Units:Minutes
Units:Peak Height Intensity
  
Analysis ID:AN002914
Laboratory Name:Fiehn Lab
Analysis Type:MS
Operator Name:Jacob Folz
Chromatography ID:CH002158
Num Factors:21
Num Metabolites:56
Rt Units:Minutes
Units:Peak Height Intensity
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