Summary of Study ST001845

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000667. The data can be accessed directly via it's Project DOI: 10.21228/M8FX07 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001845
Study TitleIdentification of unique metabolite networks between Latino and Caucasian patients with nonalcoholic fatty liver disease (NAFLD) (part V)
Study SummaryNonalcoholic fatty liver disease (NAFLD) is a spectrum of liver pathology ranging from simple steatosis to nonalcoholic steatohepatitis (NASH); the latter is characterized by inflammation and fibrosis. Risk factors for NALFD include obesity, diabetes, hyperlipidemia, and hypertension—all of which are features of metabolic syndrome. NAFLD is a very heterogeneous disease, as it presents in different patterns in males and females and in patients from different ethnicities, with unclear predictors for development and severity of disease. Previous studies have shown that NAFLD is 1.4 times more frequent in Hispanics than in Caucasians. One of the major challenges in NAFLD is the lack of accurate, noninvasive biomarkers for the detection of the most aggressive presentation, NASH. The gold standard for the diagnosis is liver biopsy, which is an invasive procedure associated with possible complications. Noninvasive diagnosis of NASH is a major unmet medical need and there are no ethnicity-specific biomarkers that can diagnose this condition and predict its progression. Therefore, the main gap in knowledge that this proposal and line of research will address is the characterizing the different plasma and liver metabolomics profile of patients with fatty liver from two ethnicities (Latinos vs. Caucasians) and of both sexes. The overall hypothesis of the present study is that the higher incidence of nonalcoholic fatty liver (NAFL) in Latino patients is reflected in a different plasma and liver metabolomics profile compared to Caucasian patients with further sex-related differences. Characterization of metabolite networks can aid in identifying the mechanistic underpinnings of sex and ethnic driven differences in NAFL which could help diagnose and establish a prognosis of this condition, especially in the critical transition from NAFL to the more aggressive nonalcoholic steatohepatitis (NASH).To address this hypothesis, plasma metabolomics profile of samples from male and female Latino and Caucasian bariatric surgery patients with NAFL and from healthy subjects will be compared. Metabolomics findings will be related with liver pathology and liver transcriptome profiles from intraoperatively obtained liver biopsies using correlation, network, and pathway analysis.
Institute
University of California, Davis
DepartmentDepartment of Internal Medicine, Division of Gastroenterology and Hepatology
LaboratoryMedici Lab
Last NameMedici
First NameValentina
Address4150 V Street - PSSB Suite 3500 - 95817 Sacramento CA
Emailvmedici@ucdavis.edu
Phone(916) 734 3751
Submit Date2021-06-21
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailGC-MS
Release Date2021-07-05
Release Version1
Valentina Medici Valentina Medici
https://dx.doi.org/10.21228/M8FX07
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000667
Project DOI:doi: 10.21228/M8FX07
Project Title:Identification of unique metabolite networks between Latino and Caucasian patients with nonalcoholic fatty liver disease (NAFLD)
Project Summary:Nonalcoholic fatty liver disease (NAFLD) is a spectrum of liver pathology ranging from simple steatosis to nonalcoholic steatohepatitis (NASH); the latter is characterized by inflammation and fibrosis. Risk factors for NALFD include obesity, diabetes, hyperlipidemia, and hypertension—all of which are features of metabolic syndrome. NAFLD is a very heterogeneous disease, as it presents in different patterns in males and females and in patients from different ethnicities, with unclear predictors for development and severity of disease. Previous studies have shown that NAFLD is 1.4 times more frequent in Hispanics than in Caucasians. One of the major challenges in NAFLD is the lack of accurate, noninvasive biomarkers for the detection of the most aggressive presentation, NASH. The gold standard for the diagnosis is liver biopsy, which is an invasive procedure associated with possible complications. Noninvasive diagnosis of NASH is a major unmet medical need and there are no ethnicity-specific biomarkers that can diagnose this condition and predict its progression. Therefore, the main gap in knowledge that this proposal and line of research will address is the characterizing the different plasma and liver metabolomics profile of patients with fatty liver from two ethnicities (Latinos vs. Caucasians) and of both sexes. The overall hypothesis of the present study is that the higher incidence of nonalcoholic fatty liver (NAFL) in Latino patients is reflected in a different plasma and liver metabolomics profile compared to Caucasian patients with further sex-related differences. Characterization of metabolite networks can aid in identifying the mechanistic underpinnings of sex and ethnic driven differences in NAFL which could help diagnose and establish a prognosis of this condition, especially in the critical transition from NAFL to the more aggressive nonalcoholic steatohepatitis (NASH).To address this hypothesis, plasma metabolomics profile of samples from male and female Latino and Caucasian bariatric surgery patients with NAFL and from healthy subjects will be compared. Metabolomics findings will be related with liver pathology and liver transcriptome profiles from intraoperatively obtained liver biopsies using correlation, network, and pathway analysis.
Institute:University of California, Davis
Department:Department of Internal Medicine, Division of Gastroenterology and Hepatology
Laboratory:Medici Lab
Last Name:Medici
First Name:Valentina
Address:GI and Hepatology Division Academic Office - 4150 V Street - PSSB Suite 3500 - 95817 Sacramento CA
Email:vmedici@ucdavis.edu
Phone:(916) 734 3751

Subject:

Subject ID:SU001922
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:23-73
Gender:Male and female
Human Race:Hispanic and Caucasian

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id factor
SA171645840422-28_021N / A
SA171646840422-20_014NAFLD-CAU
SA171647840422-19_013NAFLD-CAU
SA171648840422-24_016NAFLD-CAU
SA171649840422-27_018NAFLD-CAU
SA171650840422-30_019NAFLD-CAU
SA171651840422-01_001NAFLD-CAU
SA171652840422-25_017NAFLD-CAU
SA171653840422-17_011NAFLD-CAU
SA171654840422-16_010NAFLD-CAU
SA171655840422-08_006NAFLD-CAU
SA171656840422-04_003NAFLD-CAU
SA171657840422-13_008NAFLD-CAU
SA171658840422-03_002NAFLD-CAU
SA171659840422-14_009NAFLD-CAU
SA171660840422-05_004NAFLD-CAU
SA171661840422-44_020NAFLD-HIS
SA171662840422-10_007NAFLD-HIS
SA171663840422-18_012NAFLD-HIS
SA171664840422-21_015NAFLD-HIS
SA171665840422-07_005NAFLD-HIS
Showing results 1 to 21 of 21

Collection:

Collection ID:CO001915
Collection Summary:Blood was collected as a part of a routine/pre-operation check up, not more than 2 weeks prior to operation day (bariatric surgery) for the NAFLD group and among normal BMI healthy volunteers for the control group. All volunteers were fasted 10-12 hours before collection. Liver obtained during surgery (no preservatives, flash-frozen)
Sample Type:Blood

Treatment:

Treatment ID:TR001934
Treatment Summary:Subjects were divided into two groups either: Healthy control or Nonalcoholic fatty liver disease (NAFLD)

Sample Preparation:

Sampleprep ID:SP001928
Sampleprep Summary:1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis

Combined analysis:

Analysis ID AN002988
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 4000 QTrap
Ion Mode UNSPECIFIED
Units nM

Chromatography:

Chromatography ID:CH002217
Chromatography Summary:Targeted oxylipin analysis
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS002778
Analysis ID:AN002988
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:analyzed by a Waters UPLC with a 2.1×150 mm, 1.7 µm Acquity BEH column, ESI (+) and (-) switching and detection by multi-reaction monitoring on an Sciex 6500+ QTRAP mass spectrometer. We validated this method for linearity, limit of detection, limit of quantitation, inter- and intra-day reproducibility, and recovery for all targets, yielding better than 15% relative standard deviation and (spiked) recoveries of greater than 80%.
Ion Mode:UNSPECIFIED
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