Summary of Study ST001906

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001200. The data can be accessed directly via it's Project DOI: 10.21228/M8KX3M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001906
Study TitleMetabolomic profiling of plasma in diabetes patients
Study SummaryWe performed comprehensive profiling of plasma and salivary metabolomes, and investigated multivariate covariations with clinical markers of oral and cardiometabolic health in healthy subjects and type 2 diabetes patients. The key findings highlight the potential utility of salivary metabolites for reflecting cardiometabolic changes, including hyperglycemia and dyslipidemia. Our study shows that analysis of panels of salivary metabolites may become an attractive alternative to blood testing for screening of metabolic disorders.
Institute
Osaka University
Last NameSakanaka
First NameAkito
Address1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Emailsakanaka@dent.osaka-u.ac.jp
Phone+81668792922
Submit Date2021-08-15
Analysis Type DetailGC-MS
Release Date2022-12-09
Release Version1
Akito Sakanaka Akito Sakanaka
https://dx.doi.org/10.21228/M8KX3M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001200
Project DOI:doi: 10.21228/M8KX3M
Project Title:Study on the correlation between saliva and plasma in glucose intolerance
Project Summary:Metabolomic profiling of plasma and saliva in type 2 diabetes patients was performed.
Institute:Osaka University
Last Name:Sakanaka
First Name:Akito
Address:Yamadaoka 1-8, Suita, Osaka, 565-0871, Japan
Email:sakanaka@dent.osaka-u.ac.jp
Phone:+81668792922

Subject:

Subject ID:SU001984
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diagnosis
SA176673C09Control
SA176674C10Control
SA176675C08Control
SA176676C28Control
SA176677C26Control
SA176678C17Control
SA176679C23Control
SA176680C25Control
SA176681C03Control
SA176682C18Control
SA176683C24Control
SA176684C04Control
SA176685C14Control
SA176686C12Control
SA176687C05Control
SA176688C11Control
SA176689C06Control
SA176690C19Control
SA176691C29Control
SA176692C07Control
SA176693C16Control
SA176694C20Control
SA176695C30Control
SA176696C13Control
SA176697C22Control
SA176698C01Control
SA176699C27Control
SA176700C02Control
SA176701C15Control
SA176702C21Control
SA176703P14BDiabetes
SA176704P09BDiabetes
SA176705P27BDiabetes
SA176706P31BDiabetes
SA176707P03BDiabetes
SA176708P33BDiabetes
SA176709P04BDiabetes
SA176710P05BDiabetes
SA176711P02BDiabetes
SA176712P21BDiabetes
SA176713P26BDiabetes
SA176714P07BDiabetes
SA176715P12BDiabetes
SA176716P24BDiabetes
SA176717P20BDiabetes
SA176718P23BDiabetes
SA176719P08BDiabetes
SA176720P30BDiabetes
SA176721P29BDiabetes
SA176722P19BDiabetes
SA176723P22BDiabetes
SA176724P10BDiabetes
SA176725P01BDiabetes
SA176726P25BDiabetes
SA176727P17BDiabetes
SA176728P11BDiabetes
SA176729P15BDiabetes
SA176730P16BDiabetes
SA176731P13BDiabetes
SA176732P06BDiabetes
SA176733P32BDiabetes
Showing results 1 to 61 of 61

Collection:

Collection ID:CO001977
Collection Summary:Fasting plasma used for metabolomics was collected, kept at 4℃ in a freezer (CubeCooler®; Forte Grow Medical, Tochigi, Japan), and then subsequently frozen at −80℃
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001996
Treatment Summary:Systemically healthy controls and T2D patients were recruited.

Sample Preparation:

Sampleprep ID:SP001990
Sampleprep Summary:At first, 50 µL of plasma was mixed with 150 µL of deaerated H2O containing 0.2 mg/mL of ribitol, which was used as an internal standard, (1200 rpm, 10 min, 4°C). Then, 800 µL of deaerated MeCN was added (1200 rpm, 10 min, 4°C) and centrifuged (16000×g, 3 min, 4°C). Next, 300 µL of supernatant were transferred to an Eppendorf tube. All the steps up to this point were performed in a cold room at 4°C. The samples were then dried in a vacuum centrifuge dryer for 1 hour and lyophilized overnight. For derivatization, first, 100 µL of methoxyamine hydrochloride in pyridine (20 mg/mL) was added to the samples, and the mixture was incubated (1200 rpm, 90 min, 30°C). A second derivatizing agent, N-methyl-N-trimethysilyl-trifuroacetamide (MSTFA), was then added and the mixture was incubated (1200 rpm, 30 min, 37°C). After centrifucation (16000×g, 3 min), 100 µL of derivatized samples were transferred to glass vials.

Combined analysis:

Analysis ID AN003103
Analysis type MS
Chromatography type GC
Chromatography system GC-MS/MS-TQ8040
Column InertCap 5MS/NP capillary
MS Type EI
MS instrument type Triple quadrupole
MS instrument name Shimazu TQ8040
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH002290
Instrument Name:GC-MS/MS-TQ8040
Column Name:InertCap 5MS/NP capillary
Chromatography Type:GC

MS:

MS ID:MS002885
Analysis ID:AN003103
Instrument Name:Shimazu TQ8040
Instrument Type:Triple quadrupole
MS Type:EI
MS Comments:GC-MS data were converted into ABF format using an ABF converter, then processed using MS-DIAL (version 3.90) to perform feature detection, spectra deconvolution, metabolite identification, and peak alignment (Tsugawa et al. 2015). Normalization was then performed based on the internal standard (ribitol) as well as the LOWESS algorithm, whereby metabolic feature signal drift with time was independently corrected by fitting a LOWESS curve to the MS signal measured in QCs.
Ion Mode:POSITIVE
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