Summary of Study ST001910

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001204. The data can be accessed directly via it's Project DOI: 10.21228/M82X2K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001910
Study TitleImpact of host dietary BCAA to gut symbiont-derived lipid profile
Study SummaryWe analyzed mouse feces samples of C57BL/6 mice from diet intervention experiment. Briefly, mice were fed with BCAA-sufficient formulated diet (Testdiet 5CC7) for seven days, followed by BCAA-deficient formulated diet (Testdiet 58ZX) for the following seven days. Fecal sample were collected from mice at baseline (n=11), at day 7 (n=10) and at day 14 (n=10).
Institute
Brigham and Women's Hospital
Last NameSungwhan
First NameOh
Address60 Fenwood Rd, BTM 3016
Emailsungwhan_oh@hms.harvard.edu
Phone6175255009
Submit Date2021-07-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-01-18
Release Version1
Oh Sungwhan Oh Sungwhan
https://dx.doi.org/10.21228/M82X2K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001204
Project DOI:doi: 10.21228/M82X2K
Project Title:Metabolomic and lipidomic analysis of mouse microbiome-associated samples
Project Summary:This project includes metabolomic and lipidomic analysis of samples originated from gnotobiotic mice.
Institute:Brigham and Women's Hospital
Last Name:Sungwhan
First Name:Oh
Address:60 Fenwood Rd, BTM 3016
Email:sungwhan_oh@hms.harvard.edu
Phone:6175255009

Subject:

Subject ID:SU001988
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Swiss-Webster

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Datapoint
SA176918baseline_#5base
SA176919baseline_#4base
SA176920baseline_#2base
SA176921baseline_#6base
SA176922baseline_#3base
SA176923baseline_#9base
SA176924baseline_#11base
SA176925baseline_#10base
SA176926baseline_#1base
SA176927baseline_#8base
SA176928baseline_#7base
SA176898BCAA_D14_#4Day 14
SA176899BCAA_D14_#3Day 14
SA176900BCAA_D14_#1Day 14
SA176901BCAA_D14_#6Day 14
SA176902BCAA_D14_#2Day 14
SA176903BCAA_D14_#5Day 14
SA176904BCAA_D14_#9Day 14
SA176905BCAA_D14_#8Day 14
SA176906BCAA_D14_#10Day 14
SA176907BCAA_D14_#7Day 14
SA176908BCAA_D7_#7Day 7
SA176909BCAA_D7_#8Day 7
SA176910BCAA_D7_#10Day 7
SA176911BCAA_D7_#6Day 7
SA176912BCAA_D7_#9Day 7
SA176913BCAA_D7_#5Day 7
SA176914BCAA_D7_#2Day 7
SA176915BCAA_D7_#3Day 7
SA176916BCAA_D7_#1Day 7
SA176917BCAA_D7_#4Day 7
Showing results 1 to 31 of 31

Collection:

Collection ID:CO001981
Collection Summary:Fecal pellets (20-40mg) were collected and extracted by two phase extraction of MTBE-MeOH-water.
Sample Type:Feces

Treatment:

Treatment ID:TR002000
Treatment Summary:BCAA-sufficient (2.7% combined Leu, Ile and Val), formulated diet was given for first 7 days, followed by BCAA-deficient formulated diet.

Sample Preparation:

Sampleprep ID:SP001994
Sampleprep Summary:Samples were extracted by two phase extraction of MTBE-MeOH-water.

Combined analysis:

Analysis ID AN003107
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Agilent Eclipse C18 (75 x 4.6mm, 1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE
Units count per second

Chromatography:

Chromatography ID:CH002294
Chromatography Summary:Reverse-phase C18
Instrument Name:Thermo Vanquish
Column Name:Agilent Eclipse C18 (75 x 4.6mm, 1.8um)
Column Temperature:40
Flow Gradient:ACN:2-propanol:water=65:25:10 to 10:80:10, 0.05% formic acid
Flow Rate:600uL/min
Solvent A:65% acetonitrile/25% isopropanol/10% water; 0.05% formic acid
Solvent B:10% acetonitrile/80% isopropanol/10% water; 0.05% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS002889
Analysis ID:AN003107
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:spray voltage, 3kV sheath gas, 60AU auxiliary gas, 15AU capillary temperature, 320°C probe heater temperature, 400°C mean collision energy, 22.5AU.
Ion Mode:NEGATIVE
  logo