Summary of Study ST001915

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001208. The data can be accessed directly via it's Project DOI: 10.21228/M8JX2X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001915
Study TitleMyocardial Rev-erb-mediated diurnal metabolic rhythm( Part1/3)
Study SummaryAgonists and antagonists of nuclear receptor Rev-erbα/β, key components of the circadian clock, can benefit the heart. Here, we show that mice with cardiomyocyte-specific knockout (KO) of Rev-erbα/β display progressive cardiac dilation and lethal heart failure. Inducible ablation of Rev-erbα/β in adult hearts causes similar phenotypes. Impaired fatty acid oxidation in the KO myocardium, particularly in the light cycle, precedes contractile dysfunctions with a reciprocal overreliance on carbohydrate utilization, particularly in the dark cycle. These findings delineate temporal coordination between clock-mediated anticipation and nutrient-induced response in myocardial metabolism.
Institute
Baylor College of Medicine
Last NameSong
First NameShiyang
AddressOne Baylor Plaza, Houston, Texas, 77030, USA
Emailshiyangs@bcm.edu
Phone7137983159
Submit Date2021-09-10
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2022-12-24
Release Version1
Shiyang Song Shiyang Song
https://dx.doi.org/10.21228/M8JX2X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001208
Project DOI:doi: 10.21228/M8JX2X
Project Title:Myocardial Rev-erb-mediated diurnal metabolic rhythm
Project Summary:Agonists and antagonists of nuclear receptor Rev-erbα/β, key components of the circadian clock, can benefit the heart. Here, we show that mice with cardiomyocyte-specific knockout (KO) of Rev-erbα/β display progressive cardiac dilation and lethal heart failure. Inducible ablation of Rev-erbα/β in adult hearts causes similar phenotypes. Impaired fatty acid oxidation in the KO myocardium, particularly in the light cycle, precedes contractile dysfunctions with a reciprocal overreliance on carbohydrate utilization, particularly in the dark cycle. These findings delineate temporal coordination between clock-mediated anticipation and nutrient-induced response in myocardial metabolism.
Institute:Baylor College of Medicine
Last Name:Song
First Name:Shiyang
Address:One Baylor Plaza, Houston, Texas, 77030, USA
Email:shiyangs@bcm.edu
Phone:7137983159

Subject:

Subject ID:SU001993
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Group Genotype Experimental variables
SA177703KO_ZT10_3KO_ZT10 Rev-erb cardiomyocyte specific knock out KO mouse heart ventrcle harvest at ZT10
SA177704KO_ZT10_1KO_ZT10 Rev-erb cardiomyocyte specific knock out KO mouse heart ventrcle harvest at ZT10
SA177705KO_ZT10_2KO_ZT10 Rev-erb cardiomyocyte specific knock out KO mouse heart ventrcle harvest at ZT10
SA177706KO_ZT22_2KO_ZT22 Rev-erb cardiomyocyte specific knock out KO mouse heart ventrcle harvest at ZT22
SA177707KO_ZT22_1KO_ZT22 Rev-erb cardiomyocyte specific knock out KO mouse heart ventrcle harvest at ZT22
SA177708KO_ZT22_3KO_ZT22 Rev-erb cardiomyocyte specific knock out KO mouse heart ventrcle harvest at ZT22
SA177709WT_ZT10_1WT_ZT10 wild type control WT mouse heart ventrcle harvest at ZT10
SA177710WT_ZT10_3WT_ZT10 wild type control WT mouse heart ventrcle harvest at ZT10
SA177711WT_ZT10_2WT_ZT10 wild type control WT mouse heart ventrcle harvest at ZT10
SA177712WT_ZT22_1WT_ZT22 wild type control WT mouse heart ventrcle harvest at ZT22
SA177713WT_ZT22_2WT_ZT22 wild type control WT mouse heart ventrcle harvest at ZT22
SA177714WT_ZT22_3WT_ZT22 wild type control WT mouse heart ventrcle harvest at ZT22
Showing results 1 to 12 of 12

Collection:

Collection ID:CO001986
Collection Summary:Snap-frozen heart ventricles were collected from both WT and Rev-erb cardiomyocyte-specific KO mice at ZT10 or ZT22
Sample Type:Heart

Treatment:

Treatment ID:TR002005
Treatment Summary:no special treatment

Sample Preparation:

Sampleprep ID:SP001999
Sampleprep Summary:Heart ventricle tissues were harvested from male mice at 3 months old (n = 3 at each condition) and were snap-frozen in liquid nitrogen. Lipids were extracted as previously described 55–57. Lipids were separated on a Shimadzu CTO-20A Nexera X2 UHPLC systems, 1.8 μm particle 50 × 2.1 mm Acquity HSS UPLC T3 column (Waters, Milford, MA). Lipidomics acquisition performed in both pos and neg mode ionization. Pos mode lipids were normalized by Internal Standard PC 17:0; [M+H]+, Neg mode lipids were normalized by Internal Standard PC 34:0.

Combined analysis:

Analysis ID AN003113
Analysis type MS
Chromatography type GC
Chromatography system Shimadzu Nexera X2
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type EI
MS instrument type Other
MS instrument name Shimadzu QP2010 Plus
Ion Mode UNSPECIFIED
Units pmoles/l

Chromatography:

Chromatography ID:CH002298
Instrument Name:Shimadzu Nexera X2
Column Name:Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:GC

MS:

MS ID:MS002894
Analysis ID:AN003113
Instrument Name:Shimadzu QP2010 Plus
Instrument Type:Other
MS Type:EI
MS Comments:Statistical analyses were performed with either ANOVA or t-test in R Studio (R Studio Inc., Boston, MA). Differential metabolites were identified by adjusting the p-values for multiple testing at an FDR (Benjamini Hochberg method) threshold of <0.25.
Ion Mode:UNSPECIFIED
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