Summary of Study ST001927
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001217. The data can be accessed directly via it's Project DOI: 10.21228/M8D693 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001927 |
| Study Title | Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach. |
| Study Type | Untargeted Metabolomics |
| Study Summary | Entomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previosly. |
| Institute | Universidade Federal do Paraná |
| Department | Patologia Básica |
| Laboratory | Laboratório de Microbiologia e Biologia Molecular |
| Last Name | Stuart |
| First Name | Andressa |
| Address | Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil |
| andressa.katiski@gmail.com | |
| Phone | 5541991922779 |
| Submit Date | 2021-09-29 |
| Num Groups | 3 |
| Total Subjects | 15 |
| Study Comments | Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, raw(Waters) |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2022-10-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001217 |
| Project DOI: | doi: 10.21228/M8D693 |
| Project Title: | Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach. |
| Project Type: | Untargeted Metabolomics |
| Project Summary: | Entomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previously. |
| Institute: | Universidade Federal do Paraná |
| Department: | Patologia Básica |
| Laboratory: | Laboratório de Microbiologia e Biologia Molecular |
| Last Name: | Stuart |
| First Name: | Andressa |
| Address: | Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil |
| Email: | andressa.katiski@gmail.com |
| Phone: | 5541991922779 |
| Project Comments: | Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control. |
| Contributors: | Jason Lee Furuie, Thais Regiani Cataldi, Rodrigo Makowiecky Stuart, Maria Aparecida Cassilha Zawadneak, Carlos Alberto Labate, Ida Chapaval Pimentel |
Subject:
| Subject ID: | SU002005 |
| Subject Type: | Fungi |
| Subject Species: | Beauveria bassiana |
| Genotype Strain: | GenBank: KU751847; KU751848 |
Factors:
Subject type: Fungi; Subject species: Beauveria bassiana (Factor headings shown in green)
| mb_sample_id | local_sample_id | Fungal species |
|---|---|---|
| SA178405 | Bov2_1 | Beauveria bassiana Strain Bov2 |
| SA178406 | Bov2_5 | Beauveria bassiana Strain Bov2 |
| SA178407 | Bov2_4 | Beauveria bassiana Strain Bov2 |
| SA178408 | Bov2_2 | Beauveria bassiana Strain Bov2 |
| SA178409 | Bov2_3 | Beauveria bassiana Strain Bov2 |
| SA178410 | Bov3_4 | Beauveria bassiana Strain Bov3 |
| SA178411 | Bov3_5 | Beauveria bassiana Strain Bov3 |
| SA178412 | Bov3_3 | Beauveria bassiana Strain Bov3 |
| SA178413 | Bov3_2 | Beauveria bassiana Strain Bov3 |
| SA178414 | Bov3_1 | Beauveria bassiana Strain Bov3 |
| SA178415 | Consortium_4 | Consortium formed by Bov2 and Bov3 strains |
| SA178416 | Consortium_5 | Consortium formed by Bov2 and Bov3 strains |
| SA178417 | Consortium_3 | Consortium formed by Bov2 and Bov3 strains |
| SA178418 | Consortium_1 | Consortium formed by Bov2 and Bov3 strains |
| SA178419 | Consortium_2 | Consortium formed by Bov2 and Bov3 strains |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO001998 |
| Collection Summary: | Two genetically distinct strains of Beauveria bassiana (Bov 3 and Bov 2) were cultivated both separately and co-cultivated to form a fungal consortium. After the colonies had grown, the mycelium of each treatment (Bov 2, Bov 3, and the fungal consortium) was scraped from the culture medium with a spatula and then macerated separately in liquid nitrogen (N2) |
| Collection Protocol Filename: | MetaboliteExtraction |
| Sample Type: | Fungal mycelium |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002017 |
| Treatment Summary: | Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Petri dishes containing Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The cultures were incubated in the dark in a biological oxygen demand (BOD) oven for 14 days at 28°C. |
Sample Preparation:
| Sampleprep ID: | SP002011 |
| Sampleprep Summary: | Extraction was performed in microtubes, from 200 mg of fungal macerate to which 1 mL of 6:2:2 methanol:chloroform:water ice-cold extraction solution was added. These extraction microtubes were vigorously vortexed and placed in an ultrasonic low-temperature bath at 20 Hz s-1 for 15 min. The samples were then centrifuged (Eppendorf, Germany) at 4°C for 10 min at 14,000 rpm. Then, the supernatant was filtered using a 0.22 μm Whatman® filter (Merck, Germany) and transferred to a chromatographic vial where the extracts were lyophilized (Thermo Fischer Scientific, MA, USA) until completely dry. Finally, the lyophilized samples were resuspended in 200 μL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003133 | AN003134 | AN003135 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Waters Acquity UPLC | Waters Acquity UPLC |
| Column | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | GC x GC-TOF | QTOF | QTOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF | Waters Ultima QTOF | Waters Ultima QTOF |
| Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE |
| Units | Relative intensity | Relative intensity |
Chromatography:
| Chromatography ID: | CH002315 |
| Chromatography Summary: | (.cdf) |
| Methods Filename: | LCMSMS |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) |
| Column Temperature: | 70 - 320 ºC |
| Flow Rate: | 1 mL.min-1 |
| Injection Temperature: | 280 ºC |
| Sample Injection: | 1 uL |
| Oven Temperature: | 70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then held at this temperature for 4 min. |
| Chromatography Type: | GC |
| Chromatography ID: | CH002316 |
| Chromatography Summary: | Positive Mode (.raw) |
| Methods Filename: | LCMSMS |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| Column Temperature: | 35 ºC |
| Flow Gradient: | 95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min |
| Flow Rate: | 0.5 mL·min-1 |
| Solvent A: | Water; formic acid |
| Solvent B: | 100% acetonitrile; formic acid. |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002317 |
| Chromatography Summary: | Negative mode (.raw) |
| Methods Filename: | LCMSMS |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| Column Temperature: | 35 ºC |
| Flow Gradient: | 95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min |
| Flow Rate: | 0.5 mL·min-1 |
| Solvent A: | Water; formic acid |
| Solvent B: | 100% acetonitrile; formic acid. |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002913 |
| Analysis ID: | AN003133 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| MS Comments: | Data from GC-MS was processed using ChromaTOF 4.32 software. NIST library version 11 was used for the identification of metabolites. |
| Ion Mode: | UNSPECIFIED |
| Ion Source Temperature: | 250 ºC |
| Ionization Energy: | 70 eV |
| MS ID: | MS002914 |
| Analysis ID: | AN003134 |
| Instrument Name: | Waters Ultima QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Generated data were pre-processed using MassLynx 4.1 software |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3 kV |
| Source Temperature: | 150 ºC |
| Desolvation Gas Flow: | 550 L/hr. |
| MS ID: | MS002915 |
| Analysis ID: | AN003135 |
| Instrument Name: | Waters Ultima QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Generated data were pre-processed using MassLynx 4.1 software |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3 kV |
| Source Temperature: | 150 ºC |
| Desolvation Gas Flow: | 550 L/hr. |
