Summary of Study ST001953

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001240. The data can be accessed directly via it's Project DOI: 10.21228/M8F121 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001953
Study TitleIdentifying metabolite changes in human islets treated with Phospho-BAD mimicry and Inflammatory cytokines
Study SummaryThe goal of this study was to associate metabolite changes with protection of human islets from cell death induced by the diabetogenic stress of inflammatory cytokines. Protection of human islet viability was accomplished via enhanced glucose metabolism using phospho-BAD mimicry peptide treatment.
Institute
Harvard University
DepartmentDana-Farber Cancer Institute
LaboratoryNika N. Danial
Last NameFu
First NameAccalia
Address360 Longwood Ave., Boston, MA, 02215
Emailaccalia_fu@dfci.harvard.edu
Phone617-632-3000
Submit Date2021-10-18
Num Groups4
Total Subjects32
PublicationsFu et al. 2020, Fu et al. in press
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailMS(Dir. Inf.)
Release Date2021-11-12
Release Version1
Accalia Fu Accalia Fu
https://dx.doi.org/10.21228/M8F121
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001240
Project DOI:doi: 10.21228/M8F121
Project Title:Identifying metabolite changes in human islets treated with Phospho-BAD mimicry and Inflammatory cytokines
Project Type:Untargeted MS
Project Summary:The goal of this study was to associate metabolite changes with protection of human islets from cell death induced by the diabetogenic stress of inflammatory cytokines. Protection of human islet viability was accomplished via enhanced glucose metabolism using phospho-BAD mimicry peptide treatment.
Institute:Harvard University
Department:Dana-Farber Cancer Institute
Laboratory:Nika N. Danial
Last Name:Fu
First Name:Accalia
Address:360 Longwood Ave., Boston, MA, USA, 02215
Email:accalia_fu@dfci.harvard.edu
Phone:617-632-3000
Funding Source:R01DK078081
Publications:Fu et al. 2020, Fu et al. in press

Subject:

Subject ID:SU002031
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:20-53
Gender:Male and female
Cell Strain Details:Primary isolated human pancreatic islets
Cell Primary Immortalized:primary

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group Treatment
SA184056S12BAD SAHBA SD Cytokines
SA184057S10BAD SAHBA SD Cytokines
SA184058S9BAD SAHBA SD Cytokines
SA184059S13BAD SAHBA SD Cytokines
SA184060S11BAD SAHBA SD Cytokines
SA184061S14BAD SAHBA SD Cytokines
SA184062S16BAD SAHBA SD Cytokines
SA184063S15BAD SAHBA SD Cytokines
SA184064S4BAD SAHBA SD PBS
SA184065S3BAD SAHBA SD PBS
SA184066S5BAD SAHBA SD PBS
SA184067S8BAD SAHBA SD PBS
SA184068S2BAD SAHBA SD PBS
SA184069S7BAD SAHBA SD PBS
SA184070S6BAD SAHBA SD PBS
SA184071S1BAD SAHBA SD PBS
SA184072V12Vehicle Cytokines
SA184073V11Vehicle Cytokines
SA184074V9Vehicle Cytokines
SA184075V13Vehicle Cytokines
SA184076V10Vehicle Cytokines
SA184077V14Vehicle Cytokines
SA184078V16Vehicle Cytokines
SA184079V15Vehicle Cytokines
SA184080V4Vehicle PBS
SA184081V3Vehicle PBS
SA184082V5Vehicle PBS
SA184083V2Vehicle PBS
SA184084V7Vehicle PBS
SA184085V1Vehicle PBS
SA184086V8Vehicle PBS
SA184087V6Vehicle PBS
Showing results 1 to 32 of 32

Collection:

Collection ID:CO002024
Collection Summary:Untargeted MS with annotated ions and their signal intensities
Collection Protocol ID:Methods Fu et al. FIA_MS
Collection Protocol Filename:Methods Fu et al. FIA_MS
Sample Type:Cultured cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002043
Treatment Summary:Human islets were treated with vehicle DMSO 0.5% or 5uM peptide BAD SAHBa SD for 5 hours, then treated with PBS as control and inflammatory cytokines as experimental groups.
Treatment Protocol Filename:Methods Fu et al. FIA_MS
Treatment Compound:BAD SAHB SD peptide, inflammatory cytokines
Treatment Dose:5 uM peptide, 10 ng/ml TNF-α, 10 ng/ml IL-1β, and 100 ng/ml IFN
Treatment Doseduration:24 hours
Treatment Vehicle:DMSO and PBS
Cell Media:PIMS, Prodo Labs

Sample Preparation:

Sampleprep ID:SP002037
Sampleprep Summary:150 human islets per replicate were extracted in hot 70% ethanol at 75˚C for 3 min, placed on ice, and clarified by centrifugation. Extracts from all donors were pooled and analyzed in 8 replicates by flow-injections.
Sampleprep Protocol Filename:Methods Fu et al. FIA_MS
Extraction Method:Islet were extracted with hot 70% ethanol at 75˚C for 3 min

Combined analysis:

Analysis ID AN003178
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system Agilent 6550 Quadrupole TOF MS
Column none
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6550 QTOF
Ion Mode NEGATIVE
Units Ion signal intensity

Chromatography:

Chromatography ID:CH002350
Instrument Name:Agilent 6550 Quadrupole TOF MS
Column Name:none
Sample Injection:1.5 ul
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002956
Analysis ID:AN003178
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Data acquisitions performed as a service of General Metabolics, processing and ion identification/ annotations performed with proprietary of General Metabolics.
Ion Mode:NEGATIVE
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