Summary of Study ST001955

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001242. The data can be accessed directly via it's Project DOI: 10.21228/M85H78 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001955
Study TitleMetabonomics analysis reveals the physiological mechanism of promoting maize shoots growth under negative pressure to stabilize soil water content
Study SummaryThe purpose of this study is to analyze maize shoots growth under negative pressure to stabilize soil water content，Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.
Institute
Heilongjiang Bayi Agricultural University
Last NameZhang
First NameJili
AddressHigh tech Zone, Longfei District, Daqing, Heilongjiang, 163319, China
Emailzhangjili12@163.com
Phone+86-13504899312
Submit Date2021-10-03
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailAPCI-MS
Release Date2021-12-15
Release Version1
Jili Zhang Jili Zhang
https://dx.doi.org/10.21228/M85H78
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001242
Project DOI:doi: 10.21228/M85H78
Project Title:Metabonomics analysis reveals the physiological mechanism of promoting maize shoots growth under negative pressure to stabilize soil water content
Project Summary:The purpose of this study is to analyze maize shoots growth under negative pressure to stabilize soil water content，Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.
Institute:Heilongjiang Bayi Agricultural University
Last Name:Zhang
First Name:Jili
Address:High tech Zone, Longfei District, Daqing, Heilongjiang, 163319, China
Email:zhangjili12@163.com
Phone:86-13504899312

Subject:

Subject ID:SU002033
Subject Type:Plant
Subject Species:Zea mays
Taxonomy ID:4577

Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA184114DW3control
SA184115DW2control
SA184116DW4control
SA184117DW5control
SA184118DW6control
SA184119DW1control
SA184108SW3Moisture treatment
SA184109SW4Moisture treatment
SA184110SW6Moisture treatment
SA184111SW2Moisture treatment
SA184112SW5Moisture treatment
SA184113SW1Moisture treatment
Showing results 1 to 12 of 12

Collection:

Collection ID:CO002026
Collection Summary:A total of 121 differential metabolites (75 were increased and 46 were decreased) were identified in maize leaves cultivated under conditions of stable soil water content and dry-wet cycles.
Sample Type:new

Treatment:

Treatment ID:TR002045
Treatment Summary:Maize plants were subjected to two irrigation treatments. The first treatment was soil moisture dry-wet cycles, which was obtained using drip irrigation (control, DW). The second treatment was negative pressure to stabilize soil water content treatment (SW), which was obtained using the negative pressure irrigation (NPI) system.

Sample Preparation:

Sampleprep ID:SP002039
Sampleprep Summary:Collected samples were thawed on ice, and metabolites were extracted from 20 µL of each sample using 120 µL of precooled 50% methanol buffer. Next, the mixture of metabolites was vortexed for 1 min, followed by incubation for 10 min at room temperature, and storing at -20°C overnight. On the next day, the mixture was centrifuged at 4,000 g for 20 min and the supernatant was subsequently transferred to 96 well plates. Samples were stored at ‐80°C prior to the LC‐MS analysis. Pooled quality control (QC) samples were also prepared by combining 10 μL of each extraction mixture

Combined analysis:

Analysis ID AN003180 AN003181
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system TripleTOF 5600 TripleTOF 5600
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type APCI APCI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units μg/100ml μg/100ml

Chromatography:

Chromatography ID:CH002352
Instrument Name:TripleTOF 5600
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS002958
Analysis ID:AN003180
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:APCI
MS Comments:XCMS software (verison 3.4.1）
Ion Mode:NEGATIVE
  
MS ID:MS002959
Analysis ID:AN003181
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:APCI
MS Comments:XCMS software (verison 3.4.1）
Ion Mode:POSITIVE
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