Summary of Study ST001961
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001248. The data can be accessed directly via it's Project DOI: 10.21228/M8D41C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001961 |
| Study Title | Metabolomics of brown adipose tissue in murine heart failure model |
| Study Type | untargeted CE-TOF/MS metabolomic profiling |
| Study Summary | Brown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the changes of metabolites, we conducted the CE-TOF/MS analysis using BAT from TAC (thoracic aortic constriction) or sham-operated mice. In BAT from TAC-operated mice, we found increase of choline and glycerophosphorylcholine and a decrease of phosphorylcholine, suggesting that BAT dysfunction induces the disorientation of choline metabolism. |
| Institute | Juntendo University |
| Department | Department of Cardiovascular Biology and Medicine |
| Last Name | Yoshida |
| First Name | Yohko |
| Address | 2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan |
| yohko105@yahoo.co.jp | |
| Phone | +81-3-3813-3111 |
| Submit Date | 2021-10-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2022-11-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001248 |
| Project DOI: | doi: 10.21228/M8D41C |
| Project Title: | Metabolomics of brown adipose tissue in murine heart failure model |
| Project Type: | untargeted CE-TOF/MS metabolomic profiling |
| Project Summary: | Brown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the changes of metabolites, we conducted the CE-TOF/MS analysis using BAT from TAC (thoracic aortic constriction) or sham-operated mice. In BAT from TAC-operated mice, we found increase of choline and glycerophosphorylcholine and a decrease of phosphorylcholine, suggesting that BAT dysfunction induces the disorientation of choline metabolism. |
| Institute: | Juntendo University |
| Department: | Department of Cardiovascular Biology and Medicine |
| Last Name: | Yoshida |
| First Name: | Yohko |
| Address: | 2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan |
| Email: | yohko105@yahoo.co.jp |
| Phone: | +81-3-3813-3111 |
Subject:
| Subject ID: | SU002041 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Group |
|---|---|---|
| SA184620 | - | - |
| SA184621 | 4B | Sham-operated |
| SA184622 | 6B | Sham-operated |
| SA184623 | 3B | Sham-operated |
| SA184624 | 1B | Sham-operated |
| SA184625 | 2B | Sham-operated |
| SA184626 | 12B | TAC-operated |
| SA184627 | 11B | TAC-operated |
| SA184628 | 9B | TAC-operated |
| SA184629 | 7B | TAC-operated |
| SA184630 | 10B | TAC-operated |
| Showing results 1 to 11 of 11 |
Collection:
| Collection ID: | CO002034 |
| Collection Summary: | Brown adipose tissue (BAT) was excised from the mice and immediately frozen in liquid nitrogen. |
| Sample Type: | Adipose tissue |
Treatment:
| Treatment ID: | TR002053 |
| Treatment Summary: | All mice were randomly allocated for surgical procedures under blinded condition. Thoracic aortic constriction (TAC) was performed in 11-week-old male mice, and sample was collected 2 weeks after surgery. Sham-operated mice underwent the same procedures, except for aortic constriction. |
Sample Preparation:
| Sampleprep ID: | SP002047 |
| Sampleprep Summary: | To extract metabolites, 40~50mg snap-frozen samples were completely homogenized by Shake Master NEO (Bio Medical Science) in 500uL of methanol containing L-methionine sulfone (Wako 502-76641), methionine sulfone (MES, Dojindo 349-01623), and D-camphor-10-sulfonic acid sodium salt (CSA, Wako 037-01032) (all at 20 μM). After adding CHCl3 (500 μl) and distilled water (200 μl) with thorough mixing, centrifugation was performed at 4,600g for 15 min at 4°C. Then the aqueous layer (300 μl) was collected and transferred to a 5-kDa cutoff filter (UltrafreeMC-PLHCC; Human Metabolome Technologies, UFC3LCCNB-HMT). Centrifugation was performed at 9,100g for 3 hours at 20°C, after which the filtrate was centrifugally concentrated and dissolved in 50μl distilled water containing reference compounds (200 μM each of 3-aminopyrrolidine and trimesate). |
Combined analysis:
| Analysis ID | AN003196 | AN003197 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent 6224 TOF | Agilent 6224 TOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH002364 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002974 |
| Analysis ID: | AN003196 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([13C isotopic ion of deprotonated acetic acid dimer (2CH3COOH - H)]-, m/z 120.03841), and ([Hexakis + deprotonated acetic acid (CH3COOH - H)]-, m/z 680.03554). Exact mass data were acquired at a rate of 1.5 spectra/s over a 50-1000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002975 |
| Analysis ID: | AN003197 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([2MeOH13Cisotope]-, m/z 66.06306), and ([Hexakis(2,2-difluoroethoxy)phosphazene]-, m/z 622.028963). Exact mass data were acquired at the rate of 1.5 cycles/s over a 50 to 1,000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites. |
| Ion Mode: | POSITIVE |
