Summary of Study ST001967

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001253. The data can be accessed directly via it's Project DOI: 10.21228/M8R98V This work is supported by NIH grant, U2C- DK119886.

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Study IDST001967
Study TitleAnalytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS:NMR/plasma
Study SummaryMetabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute
INSERM
DepartmentINSERM
Last NameMartias
First NameCecile
Address10 Boulevard tonnelé, Indre et Loire, 37000, France
Emailcecile.martias@univ-tours.fr
Phone0247366351
Submit Date2021-09-27
Analysis Type DetailNMR
Release Date2022-01-02
Release Version1
Cecile Martias Cecile Martias
https://dx.doi.org/10.21228/M8R98V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001253
Project DOI:doi: 10.21228/M8R98V
Project Title:Analytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS
Project Summary:Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute:INSERM
Department:INSERM
Last Name:Martias
First Name:Cecile
Address:10 Boulevard tonneléTours, Indre et Loire, 37000, France
Email:cecile.martias@univ-tours.fr
Phone:0247366351

Subject:

Subject ID:SU002047
Subject Type:Mammal
Subject Species:Capra hircus

Factors:

Subject type: Mammal; Subject species: Capra hircus (Factor headings shown in green)

mb_sample_id local_sample_id Protocole
SA184939P_200_P1_9P1
SA184940P_200_P1_10P1
SA184941P_200_P1_8P1
SA184942P_200_P1_1P1
SA184943P_200_P1_7P1
SA184944P_200_P1_2P1
SA184945P_200_P1_3P1
SA184946P_200_P1_4P1
SA184947P_200_P1_6P1
SA184948P_200_P1_5P1
SA184967P_200_P2_9p2
SA184968P_200_P2_10p2
SA184949P_200_P2_7P2
SA184950P_200_P2_8P2
SA184951P_200_P2_5P2
SA184952P_200_P2_6P2
SA184953P_200_P2_4P2
SA184954P_200_P2_1P2
SA184955P_200_P2_2P2
SA184956P_200_P2_3P2
SA184957P_200_P4_7P4
SA184958P_200_P4_8P4
SA184959P_200_P4_10P4
SA184960P_200_P4_6P4
SA184961P_200_P4_9P4
SA184962P_200_P4_3P4
SA184963P_200_P4_1P4
SA184964P_200_P4_5P4
SA184965P_200_P4_2P4
SA184966P_200_P4_4P4
Showing results 1 to 30 of 30

Collection:

Collection ID:CO002040
Collection Summary:Development method for the best sample preparation for goat feces
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002059
Treatment Summary:5 sample preparation were tested : P1 : Blood:MEOH:CHCL3 1:1:1 , P2: Blood:MeOH:CHCl3 1:2.5:2.5, P4: Blood:MeOH 1:8

Sample Preparation:

Sampleprep ID:SP002053
Sampleprep Summary:-

Analysis:

Analysis ID:AN003205
Analysis Type:NMR
Num Factors:4
Num Metabolites:40
Units:SI

NMR:

NMR ID:NM000219
Analysis ID:AN003205
Instrument Name:Avance III HD 600 MHz
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz
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