Summary of Study ST001969

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001253. The data can be accessed directly via it's Project DOI: 10.21228/M8R98V This work is supported by NIH grant, U2C- DK119886.

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Study IDST001969
Study TitleAnalytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS:NMR/milk
Study SummaryMetabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute
INSERM
DepartmentINSERM
Last NameMartias
First NameCecile
Address10 Boulevard tonnelé, Indre et Loire, 37000, France
Emailcecile.martias@univ-tours.fr
Phone0247366351
Submit Date2021-09-27
Analysis Type DetailNMR
Release Date2022-01-02
Release Version1
Cecile Martias Cecile Martias
https://dx.doi.org/10.21228/M8R98V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001253
Project DOI:doi: 10.21228/M8R98V
Project Title:Analytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS
Project Summary:Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute:INSERM
Department:INSERM
Last Name:Martias
First Name:Cecile
Address:10 Boulevard tonneléTours, Indre et Loire, 37000, France
Email:cecile.martias@univ-tours.fr
Phone:0247366351

Subject:

Subject ID:SU002049
Subject Type:Mammal
Subject Species:Capra hircus
Taxonomy ID:9925

Factors:

Subject type: Mammal; Subject species: Capra hircus (Factor headings shown in green)

mb_sample_id local_sample_id Protocole
SA184999L_200_P1_8P1
SA185000L_200_P1_9P1
SA185001L_200_P1_10P1
SA185002L_200_P1_1P1
SA185003L_200_P1_7P1
SA185004L_200_P1_2P1
SA185005L_200_P1_6P1
SA185006L_200_P1_3P1
SA185007L_200_P1_4P1
SA185008L_200_P1_5P1
SA185028L_200_P3_10p3
SA185009L_200_P3_7P3
SA185010L_200_P3_9P3
SA185011L_200_P3_8P3
SA185012L_200_P3_6P3
SA185013L_200_P3_5P3
SA185014L_200_P3_2P3
SA185015L_200_P3_1P3
SA185016L_200_P3_4P3
SA185017L_200_P3_3P3
SA185018L_100_P4_7P4
SA185019L_100_P4_8P4
SA185020L_100_P4_9P4
SA185021L_100_P4_6P4
SA185022L_100_P4_10P4
SA185023L_100_P4_3P4
SA185024L_100_P4_1P4
SA185025L_100_P4_5P4
SA185026L_100_P4_2P4
SA185027L_100_P4_4P4
Showing results 1 to 30 of 30

Collection:

Collection ID:CO002042
Collection Summary:Development method for the best sample preparation for goat feces
Sample Type:Feces

Treatment:

Treatment ID:TR002061
Treatment Summary:5 sample preparation were tested : P1 : Milk:MEOH:CHCL3 1:1:1 , P3: Milk:MeOH:H2O:CHCl3 1:2.5:2.5, P4: Milk:H2O:MeOH:CHCl3 1:3:4:6

Sample Preparation:

Sampleprep ID:SP002055
Sampleprep Summary:-

Analysis:

Analysis ID:AN003210
Analysis Type:NMR
Num Factors:4
Num Metabolites:35
Units:SI

NMR:

NMR ID:NM000220
Analysis ID:AN003210
Instrument Name:Avance III HD 600 MHz
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz
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