Summary of Study ST001972

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001253. The data can be accessed directly via it's Project DOI: 10.21228/M8R98V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST001972
Study TitleAnalytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS:NMR/feces
Study SummaryMetabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute
INSERM
DepartmentINSERM
Last NameMartias
First NameCecile
Address10 Boulevard tonnelé, Indre et Loire, 37000, France
Emailcecile.martias@univ-tours.fr
Phone0247366351
Submit Date2021-09-27
Analysis Type DetailNMR
Release Date2022-01-02
Release Version1
Cecile Martias Cecile Martias
https://dx.doi.org/10.21228/M8R98V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001253
Project DOI:doi: 10.21228/M8R98V
Project Title:Analytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS
Project Summary:Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute:INSERM
Department:INSERM
Last Name:Martias
First Name:Cecile
Address:10 Boulevard tonneléTours, Indre et Loire, 37000, France
Email:cecile.martias@univ-tours.fr
Phone:0247366351

Subject:

Subject ID:SU002052
Subject Type:Mammal
Subject Species:Capra hircus
Taxonomy ID:9925

Factors:

Subject type: Mammal; Subject species: Capra hircus (Factor headings shown in green)

mb_sample_id local_sample_id Protcole
SA185154Phenomet_C18_POS_F-P1-N8P1
SA185155Phenomet_C18_POS_F-P1-N9P1
SA185156Phenomet_C18_POS_F-P1-N6P1
SA185157Phenomet_C18_POS_F-P1-N1P1
SA185158Phenomet_C18_POS_F-P1-N7P1
SA185159Phenomet_C18_POS_F-P1-N5P1
SA185160Phenomet_C18_POS_F-P1-N10P1
SA185161Phenomet_C18_POS_F-P1-N3P1
SA185162Phenomet_C18_POS_F-P1-N2P1
SA185163Phenomet_C18_POS_F-P1-N4P1
SA185164Phenomet_C18_POS_F-P2-N7P2
SA185165Phenomet_C18_POS_F-P2-N8P2
SA185166Phenomet_C18_POS_F-P2-N6P2
SA185167Phenomet_C18_POS_F-P2-N9P2
SA185168Phenomet_C18_POS_F-P2-N2P2
SA185169Phenomet_C18_POS_F-P2-N5P2
SA185170Phenomet_C18_POS_F-P2-N10P2
SA185171Phenomet_C18_POS_F-P2-N1P2
SA185172Phenomet_C18_POS_F-P2-N3P2
SA185173Phenomet_C18_POS_F-P2-N4P2
SA185174Phenomet_C18_POS_F-P4-N6P4
SA185175Phenomet_C18_POS_F-P4-N7P4
SA185176Phenomet_C18_POS_F-P4-N9P4
SA185177Phenomet_C18_POS_F-P4-N8P4
SA185178Phenomet_C18_POS_F-P4-N5P4
SA185179Phenomet_C18_POS_F-P4-N4P4
SA185180Phenomet_C18_POS_F-P4-N10P4
SA185181Phenomet_C18_POS_F-P4-N1P4
SA185182Phenomet_C18_POS_F-P4-N3P4
SA185183Phenomet_C18_POS_F-P4-N2P4
SA185184Phenomet_C18_POS_F-P5-N6P5
SA185185Phenomet_C18_POS_F-P5-N7P5
SA185186Phenomet_C18_POS_F-P5-N9P5
SA185187Phenomet_C18_POS_F-P5-N5P5
SA185188Phenomet_C18_POS_F-P5-N8P5
SA185189Phenomet_C18_POS_F-P5-N4P5
SA185190Phenomet_C18_POS_F-P5-N10P5
SA185191Phenomet_C18_POS_F-P5-N1P5
SA185192Phenomet_C18_POS_F-P5-N3P5
SA185193Phenomet_C18_POS_F-P5-N2P5
SA185194Phenomet_C18_POS_F-P6-N7P6
SA185195Phenomet_C18_POS_F-P6-N9P6
SA185196Phenomet_C18_POS_F-P6-N6P6
SA185197Phenomet_C18_POS_F-P6-N8P6
SA185198Phenomet_C18_POS_F-P6-N3P6
SA185199Phenomet_C18_POS_F-P6-N1P6
SA185200Phenomet_C18_POS_F-P6-N10P6
SA185201Phenomet_C18_POS_F-P6-N2P6
SA185202Phenomet_C18_POS_F-P6-N4P6
SA185203Phenomet_C18_POS_F-P6-N5P6
Showing results 1 to 50 of 50

Collection:

Collection ID:CO002045
Collection Summary:Development method for the best sample preparation for goat feces
Sample Type:Feces

Treatment:

Treatment ID:TR002064
Treatment Summary:5 sample preparation were tested : P1 : H2O:MEOH:CHCL3 1:1:1 , P2: ACN:H2O 1:1, P4: MeOH:H2O 1:1, MeOH:H2O:ACN 1:1:1

Sample Preparation:

Sampleprep ID:SP002058
Sampleprep Summary:-

Analysis:

Analysis ID:AN003217
Analysis Type:NMR
Num Factors:5
Num Metabolites:28
Units:SI

NMR:

NMR ID:NM000221
Analysis ID:AN003217
Instrument Name:Avance III HD 600 MHz
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz
  logo