Summary of Study ST001980
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001256. The data can be accessed directly via it's Project DOI: 10.21228/M8C412 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001980 |
| Study Title | Metabolomic profiles in S. mutans, S. gordonii, and S. oralis cells treated with D-tagatose |
| Study Summary | Recent studies have shown phenotypic and metabolic heterogeneity in related species including Streptococcus oralis, a typical oral commensal bacterium, Streptococcus mutans, a cariogenic bacterium, and Streptococcus gordonii, which functions as an accessory pathogen in periodontopathic biofilm. In this study, metabolites characteristically contained in the saliva of individuals with good oral hygiene were determined, after which the effects of an identified prebiotic candidate, D-tagatose, on phenotype, gene expression, and metabolic profiles of those three key bacterial species were investigated. Examinations of the saliva metabolome of 18 systemically healthy volunteers identified salivary D-tagatose as associated with lower dental biofilm abundance in the oral cavity (Spearman’s correlation coefficient; r = -0.603, p = 0.008), then the effects of D-tagatose on oral streptococci were analyzed in vitro. In chemically defined medium (CDM) containing D-tagatose as the sole carbohydrate source, S. mutans and S. gordonii each showed negligible biofilm formation, whereas significant biofilms were formed in cultures of S. oralis. Furthermore, even in the presence of glucose, S. mutans and S. gordonii showed growth suppression and decreases in the final viable cell count in a D-tagatose concentration-dependent manner. In contrast, no inhibitory effects of D-tagatose on the growth of S. oralis were observed. To investigate species-specific inhibition by D-tagatose, the metabolomic profiles of D-tagatose-treated S. mutans, S. gordonii, and S. oralis cells were examined. The intracellular amounts of pyruvate-derived amino acids in S. mutans and S. gordonii, but not in S. oralis, such as branched-chain amino acids and alanine, tended to decrease in the presence of D-tagatose. This phenomenon indicates that D-tagatose inhibits growth of those bacteria by affecting glycolysis and its downstream metabolism. In conclusion, the present study provides evidence that D-tagatose is abundant in saliva of individuals with good oral health. Additionally, experimental results demonstrated that D-tagatose selectively inhibits growth of the oral pathogens S. mutans and S. gordonii. In contrast, the oral commensal S. oralis seemed to be negligibly affected, thus highlighting the potential of administration of D-tagatose as an oral prebiotic for its ability to manipulate the metabolism of those targeted oral streptococci. |
| Institute | Osaka University |
| Last Name | Mayumi |
| First Name | Shota |
| Address | 1-8, Yamadaoka |
| mayumi@dent.osaka-u.ac.jp | |
| Phone | +81-6-6879-2922 |
| Submit Date | 2021-09-05 |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-09-11 |
| Release Version | 1 |
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Project:
| Project ID: | PR001256 |
| Project DOI: | doi: 10.21228/M8C412 |
| Project Title: | Metabolomic profiles in S. mutans, S. gordonii, and S. oralis cells treated with D-tagatose |
| Project Summary: | Metabolomics data for Streptococcus mutans, Streptococcus gordonii, and Streptococcus oralis in the presence or absence of D-tagatose. |
| Institute: | Osaka University |
| Last Name: | Mayumi |
| First Name: | Shota |
| Address: | 1-8, Yamadaoka |
| Email: | mayumi@dent.osaka-u.ac.jp |
| Phone: | +81-6-6879-2922 |
Subject:
| Subject ID: | SU002060 |
| Subject Type: | Bacteria |
| Subject Species: | Streptococcus gordonii DL1 Challis ; Streptococcus mutans UA159 ; Streptococcus oralis ATCC9811 |
| Taxonomy ID: | - |
Factors:
Subject type: Bacteria; Subject species: Streptococcus gordonii DL1 Challis ; Streptococcus mutans UA159 ; Streptococcus oralis ATCC9811 (Factor headings shown in green)
| mb_sample_id | local_sample_id | Hours | D-tagatose (%) |
|---|---|---|---|
| SA185548 | So_control_1 | 3 | - |
| SA185549 | Sm_control_3 | 3 | - |
| SA185550 | Sm_control_4 | 3 | - |
| SA185551 | Sg_contorol_4 | 3 | - |
| SA185552 | Sg_contorol_1 | 3 | - |
| SA185553 | Sg_contorol_3 | 3 | - |
| SA185554 | Sm_control_2 | 3 | - |
| SA185555 | Sm_control_1 | 3 | - |
| SA185556 | Sg_contorol_2 | 3 | - |
| SA185557 | So_control_3 | 3 | - |
| SA185558 | So_control_2 | 3 | - |
| SA185559 | So_control_4 | 3 | - |
| SA185560 | Sg_tagatose_4 | 3 | 5 |
| SA185561 | Sg_tagatose_2 | 3 | 5 |
| SA185562 | Sg_tagatose_3 | 3 | 5 |
| SA185563 | Sg_tagatose_1 | 3 | 5 |
| SA185564 | Sm_tagatose_1 | 3 | 5 |
| SA185565 | So_tagatose_2 | 3 | 5 |
| SA185566 | So_tagatose_1 | 3 | 5 |
| SA185567 | So_tagatose_3 | 3 | 5 |
| SA185568 | So_tagatose_4 | 3 | 5 |
| SA185569 | Sm_tagatose_3 | 3 | 5 |
| SA185570 | Sm_tagatose_2 | 3 | 5 |
| SA185571 | Sm_tagatose_4 | 3 | 5 |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO002053 |
| Collection Summary: | Cells were collected and washed with Milli-Q water by centrifugation and then flash-frozen in liquid N2. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR002072 |
| Treatment Summary: | S. mutans, S. gordonii, and S. oralis cells were incubated aerobically at 37℃ in chemically defined medium containing 0.2% D-glucose without or with 5% D-tagatose. |
Sample Preparation:
| Sampleprep ID: | SP002066 |
| Sampleprep Summary: | Ribitol was added as an internal standard for normalization, then acetonitrile was added to remove protein. After thoroughly mixing the samples, centrifugation was performed. The upper 1.6 ml was evaporated in a centrifugal concentrator and freeze-dried overnight. Methoxyamine hydrochloride in pyridine and N-methyl-N-trimethylsilyltrifluoroacetamide were added to the samples as derivatizing agents, then they were thoroughly mixed and centrifuged, after which the upper 50 μl of each sample was transferred to a vial. |
Combined analysis:
| Analysis ID | AN003230 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GC-MS/MS-TQ8040 |
| Column | GL Sciences InertCap 5MS/NP capillary column (0.25 mm × 30 m, 0.25 μm) |
| MS Type | EI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu GC-MS/MS-TQ8040 |
| Ion Mode | POSITIVE |
| Units | intensity |
Chromatography:
| Chromatography ID: | CH002382 |
| Instrument Name: | Shimadzu GC-MS/MS-TQ8040 |
| Column Name: | GL Sciences InertCap 5MS/NP capillary column (0.25 mm × 30 m, 0.25 μm) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003004 |
| Analysis ID: | AN003230 |
| Instrument Name: | Shimadzu GC-MS/MS-TQ8040 |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | The selected mass range was set to 85-500 m/z with electron impact ionization. Obtained GC/MS data were analyzed with an ABF converter (https://www.reifycs.com/AbfConverter/index.html) and the MS-DIAL software package, ver. 4.60 (http://prime.psc.riken.jp/Metabolomics_Software/MS-DIAL/index.html). |
| Ion Mode: | POSITIVE |
