Summary of Study ST002028

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001287. The data can be accessed directly via it's Project DOI: 10.21228/M8BX22 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002028
Study Title	Metabolomics Analysis of Blood Plasma and Stool from Six Week Flaxseed Dietary Intervention in Postmenopausal Women (Stool/HILIC)
Study Summary	: Samples were taken before and after 6 weeks of flaxseed dietary intervention. Serum and fecal metabolomics were performed to characterize the metabolomic profiles of serum and stool metabolites pre- and post-intervention.
Institute	University of California, Davis
Last Name	folz
First Name	jake
Address	451 Health Science Drive
Email	jfolz@ucdavis.edu
Phone	7155636311
Submit Date	2021-12-21
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-01-02
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001287
Project DOI:	doi: 10.21228/M8BX22
Project Title:	Metabolomics Analysis of Blood Plasma and Stool from Six Week Flaxseed Dietary Intervention in Postmenopausal Women
Project Summary:	Samples were taken before and after 6 weeks of flaxseed dietary intervention. Serum and fecal metabolomics were performed to characterize the metabolomic profiles of serum and stool metabolites pre- and post-intervention.
Institute:	University of California, Davis
Last Name:	Folz
First Name:	jake
Address:	451 Health Science Drive
Email:	jfolz@ucdavis.edu
Phone:	7155636311

Subject:

Subject ID:	SU002110
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Treatment
SA190602	RS-01920609	Post-intervention
SA190603	RS-01916436	Post-intervention
SA190604	RS-02219083	Post-intervention
SA190605	RS-02217669	Post-intervention
SA190606	RS-01912456	Post-intervention
SA190607	RS-01923384	Post-intervention
SA190608	RS-01940439	Post-intervention
SA190609	RS-01933527	Post-intervention
SA190610	RS-02210756	Post-intervention
SA190611	RS-01909498	Post-intervention
SA190612	RS-02220151	Post-intervention
SA190613	RS-02254825	Post-intervention
SA190614	RS-02269483	Post-intervention
SA190615	RS-01865119	Post-intervention
SA190616	RS-01851707	Post-intervention
SA190617	RS-02248450	Post-intervention
SA190618	RS-01891737	Post-intervention
SA190619	RS-01950714	Post-intervention
SA190620	RS-02221392	Post-intervention
SA190621	RS-01899167	Post-intervention
SA190622	RS-01906962	Post-intervention
SA190623	RS-02209055	Post-intervention
SA190624	RS-02177788	Post-intervention
SA190625	RS-02099628	Post-intervention
SA190626	RS-02097213	Post-intervention
SA190627	RS-02179395	Post-intervention
SA190628	RS-02119956	Post-intervention
SA190629	RS-02129908	Post-intervention
SA190630	RS-02139810	Post-intervention
SA190631	RS-02136575	Post-intervention
SA190632	RS-02135615	Post-intervention
SA190633	RS-02186686	Post-intervention
SA190634	RS-02055701	Post-intervention
SA190635	RS-02207959	Post-intervention
SA190636	RS-02208413	Post-intervention
SA190637	RS-01975227	Post-intervention
SA190638	RS-02019533	Post-intervention
SA190639	RS-02193478	Post-intervention
SA190640	RS-02055250	Post-intervention
SA190641	RS-02045482	Post-intervention
SA190642	RS-02040176	Post-intervention
SA190643	RS-01838033	Post-intervention
SA190644	RS-01871547	Post-intervention
SA190645	RS-02622760	Post-intervention
SA190646	RS-01713712	Post-intervention
SA190647	RS-01707130	Post-intervention
SA190648	RS-01704567	Post-intervention
SA190649	RS-02309902	Post-intervention
SA190650	RS-02621442	Post-intervention
SA190651	RS-01735982	Post-intervention
SA190652	RS-01730343	Post-intervention
SA190653	RS-02583618	Post-intervention
SA190654	RS-02637716	Post-intervention
SA190655	RS-01694989	Post-intervention
SA190656	RS-01607340	Post-intervention
SA190657	RS-02756031	Post-intervention
SA190658	RS-01586115	Post-intervention
SA190659	RS-01574184	Post-intervention
SA190660	RS-01617214	Post-intervention
SA190661	RS-02750185	Post-intervention
SA190662	RS-02674498	Post-intervention
SA190663	RS-01686993	Post-intervention
SA190664	RS-01648850	Post-intervention
SA190665	RS-02354063	Post-intervention
SA190666	RS-01718537	Post-intervention
SA190667	RS-01776131	Post-intervention
SA190668	RS-01775777	Post-intervention
SA190669	RS-01747171	Post-intervention
SA190670	RS-01807614	Post-intervention
SA190671	RS-01830763	Post-intervention
SA190672	RS-01770486	Post-intervention
SA190673	RS-01803066	Post-intervention
SA190674	RS-01759730	Post-intervention
SA190675	RS-01764367	Post-intervention
SA190676	RS-02159560	Pre-intervention
SA190677	RS-02173570	Pre-intervention
SA190678	RS-02174019	Pre-intervention
SA190679	RS-02176985	Pre-intervention
SA190680	RS-02343753	Pre-intervention
SA190681	RS-02328916	Pre-intervention
SA190682	RS-02281190	Pre-intervention
SA190683	RS-02163124	Pre-intervention
SA190684	RS-02171917	Pre-intervention
SA190685	RS-02282708	Pre-intervention
SA190686	RS-02734868	Pre-intervention
SA190687	RS-02505149	Pre-intervention
SA190688	RS-02349304	Pre-intervention
SA190689	RS-02186695	Pre-intervention
SA190690	RS-02232959	Pre-intervention
SA190691	RS-02189596	Pre-intervention
SA190692	RS-02224703	Pre-intervention
SA190693	RS-02636728	Pre-intervention
SA190694	RS-02245717	Pre-intervention
SA190695	RS-02348498	Pre-intervention
SA190696	RS-02348451	Pre-intervention
SA190697	RS-02184855	Pre-intervention
SA190698	RS-02724295	Pre-intervention
SA190699	RS-02681370	Pre-intervention
SA190700	RS-02731752	Pre-intervention
SA190701	RS-01975879	Pre-intervention

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Collection:

Collection ID:	CO002103
Collection Summary:	: Samples were taken before and after 6 weeks of flaxseed dietary intervention. Serum and fecal metabolomics were performed to characterize the metabolomic profiles of serum and stool metabolites pre- and post-intervention.
Sample Type:	Feces

Treatment:

Treatment ID:	TR002122
Treatment Summary:	: Samples were taken before and after 6 weeks of flaxseed dietary intervention. Serum and fecal metabolomics were performed to characterize the metabolomic profiles of serum and stool metabolites pre- and post-intervention.

Sample Preparation:

Sampleprep ID:	SP002116
Sampleprep Summary:	Extraction is carried out using a bi-phasic solvent system of cold methanol, methyl tert-butyl ether (MTBE), and water. In more detail, cold methanol (225 µL is added to a 5mg tissue sample aliquot, which is placed into a 1.5 mL Eppendorf tube. Then, 750 µL of cold MTBE is added, followed by vortexing for 10 s. and shaking for 6 min. at 4ºC. Phase separation is induced by adding 188 µL of mass spec-grade water. After vortexing for 20 s. the sample is centrifuged at 14,000 rpm for 2 min. The upper organic phase is collected in two 300 µL aliquots for lipid analysis polar layer is collected in two 125 µL aliquots for HILIC analysis. One is stored at -20ºC as a backup and the other is evaporated to dryness in a SpeedVac. Dried extracts are resuspended in acetonitrile.

Sampleprep ID:

SP002116

Sampleprep Summary:

Extraction is carried out using a bi-phasic solvent system of cold methanol, methyl tert-butyl ether (MTBE), and water. In more detail, cold methanol (225 µL is added to a 5mg tissue sample aliquot, which is placed into a 1.5 mL Eppendorf tube. Then, 750 µL of cold MTBE is added, followed by vortexing for 10 s. and shaking for 6 min. at 4ºC. Phase separation is induced by adding 188 µL of mass spec-grade water. After vortexing for 20 s. the sample is centrifuged at 14,000 rpm for 2 min. The upper organic phase is collected in two 300 µL aliquots for lipid analysis polar layer is collected in two 125 µL aliquots for HILIC analysis. One is stored at -20ºC as a backup and the other is evaporated to dryness in a SpeedVac. Dried extracts are resuspended in acetonitrile.

Combined analysis:

Analysis ID	AN003298
Analysis type	MS
Chromatography type	HILIC
Chromatography system	Vanquish
Column	Waters Acquity UPLC BEH amide,(150 x 2.1mm,1.7um)
MS Type	ESI
MS instrument type	Orbitrap
MS instrument name	Thermo Q Exactive HF hybrid Orbitrap
Ion Mode	POSITIVE
Units	normalized peak height

Chromatography:

Chromatography ID:	CH002437
Instrument Name:	Vanquish
Column Name:	Waters Acquity UPLC BEH amide,(150 x 2.1mm,1.7um)
Chromatography Type:	HILIC

MS:

MS ID:	MS003068
Analysis ID:	AN003298
Instrument Name:	Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	A Thermo Q-Exactive HF Orbitrap MS instrument was operated in positive and negative ESI mdoes respectively with the following parameters: mass range 60−900 m/z; spray voltage 3.6kV (ESI+) and −3kV (ESI−), sheath gas (nitrogen) flow rate 60 units; auxiliary gas (nitrogen) flow rate 25 units, capillary temperature 320 ◦C, full scan MS1 mass resolving power 60,000, data-dependent MSMS (dd-MSMS) 4 scans per cycle, normalized collision energy at 20%, 30%, and 40%, dd-MSMS mass resolving power 15,000. Thermo Xcalibur 4.0.27.19 was used for data acquisition and analysis. Instruments was tuned and calibrated by manufacturer’s recommendations.
Ion Mode:	POSITIVE