Summary of Study ST002071
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001313. The data can be accessed directly via it's Project DOI: 10.21228/M80H5Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002071 |
| Study Title | Metabolic profiling of mouse CD27+ and CD27- gammadelta T cells |
| Study Summary | Cultured CD27+ and CD27- gammadelta T cells were labeled with 13C-glucose for 24 h. Cells were harvested for LC-MS analysis |
| Institute | University of Louisville |
| Last Name | Zhang |
| First Name | Xiang |
| Address | Shumaker Research Bld 349, Louisville, KY 40292 |
| xiang.zhang@louisville.edu | |
| Phone | 502-852-8878 |
| Submit Date | 2022-01-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-02-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001313 |
| Project DOI: | doi: 10.21228/M80H5Z |
| Project Title: | Metabolic profiling of mouse CD27+ and CD27- gammadelta T cells |
| Project Type: | Research |
| Project Summary: | The objective of this study is to define metabolic profiles of innate mouse CD27+ and CD27- gammadelta T cells. |
| Institute: | University of Louisville |
| Last Name: | Yan |
| First Name: | Jun |
| Address: | 505 S Hancock Street, Louisville, KY 40245 |
| Email: | jun.yan@louisville.edu |
| Phone: | 502 852-3628 |
| Funding Source: | R01AI161106 |
Subject:
| Subject ID: | SU002153 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 6-8 weeks old |
| Weight Or Weight Range: | 15-20 grams |
| Gender: | Male and female |
| Cell Strain Details: | C57Bl/6 |
| Species Group: | Mammals |
Factors:
Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cell type |
|---|---|---|
| SA194771 | 0412_CD27+_Labeled_3 | CD27+ |
| SA194772 | 0426_CD27+_Labeled_1 | CD27+ |
| SA194773 | 0426_CD27+_Labeled_3 | CD27+ |
| SA194774 | 0412_CD27+_Labeled_2 | CD27+ |
| SA194775 | 0426_CD27+_Labeled_2 | CD27+ |
| SA194776 | 0412_CD27+_Labeled_1 | CD27+ |
| SA194777 | 0412_CD27-_Labeled_2 | CD27- |
| SA194778 | 0426_CD27-_Labeled_1 | CD27- |
| SA194779 | 0426_CD27-_Labeled_2 | CD27- |
| SA194780 | 0426_CD27-_Labeled_3 | CD27- |
| SA194781 | 0412_CD27-_Labeled_1 | CD27- |
| Showing results 1 to 11 of 11 |
Collection:
| Collection ID: | CO002146 |
| Collection Summary: | polarized CD27+ and CD27- γδ T cells were cultured in glucose-free DMEM supplemented with 10 mM [U-13C]-glucose and 10% dialyzed FBS for 24 h. The cells were rinsed in cold PBS and quenched using a mixture containing 2 mL acetonitrile and 1.5 mL H2O. After adding 1 mL chloroform, the sample was homogenized and centrifuged at 3,000 rpm, 4 oC for 20 min. The top layer was transferred to a new tube and lyophilized. The dried sample was then dissolved in 100 µL 20% acetonitrile and vigorously vortex-mixed for 3 min. After centrifugation at 14,000 rpm, 4oC for 20 min, the supernatant was collected for two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) analysis. |
| Sample Type: | T-cells |
Treatment:
| Treatment ID: | TR002165 |
| Treatment Summary: | Cells were not treated with any drugs |
Sample Preparation:
| Sampleprep ID: | SP002159 |
| Sampleprep Summary: | The cells were rinsed in cold PBS and quenched using a mixture containing 2 mL acetonitrile and 1.5 mL H2O. After adding 1 mL chloroform, the sample was homogenized and centrifuged at 3,000 rpm, 4 oC for 20 min. The top layer was transferred to a new tube and lyophilized. The dried sample was then dissolved in 100 µL 20% acetonitrile and vigorously vortex-mixed for 3 min. After centrifugation at 14,000 rpm, 4oC for 20 min, the supernatant was collected for two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) analysis. All samples were analyzed on a Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled with a Thermo DIONEX UltiMate 3000 HPLC system (Thermo Fisher Scientific, Waltham, MA, USA). The UltiMate 3000 HPLC system was equipped with a reversed phase chromatography (RPC) column and a hydrophilic interaction chromatography (HILIC) column. The two columns were configured in parallel 2DLC mode. |
Chromatography:
| Chromatography ID: | CH002495 |
| Chromatography Summary: | We use parallel 2DLC-MS, one column is HILIC and another is RP. |
| Instrument Name: | Q Exactive |
| Column Name: | SeQuant ZIC-cHILIC |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN003376 |
| Analysis Type: | MS |
| Chromatography ID: | CH002495 |
| Num Factors: | 2 |
| Num Metabolites: | 21 |
| Units: | None |
