Summary of Study ST002144
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001358. The data can be accessed directly via it's Project DOI: 10.21228/M85X2H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002144 |
| Study Title | Structure-based non-targeted mass spectrometry imaging analysis of dried long pepper |
| Study Summary | The following is modified from the abstract of the manuscript by Wisman et al.: The dried long pepper was analyzed by MALDI-MSI IT-TOF for non-targeted analysis. Principal component analysis (PCA) was employed to discriminate between the seed and pericarp regions, and mine potential important compounds related to both regions. Investigation of these peaks revealed slight but potentially important distribution differences between them, despite sharing the same accumulation profile in a region. Furthermore, piperamides such as piperine and piperlonguminine were among the compounds closely associated with the pericarp region. This is the first study to report the utilization of PCA for spatial feature extraction and the localization of piperamides in dried long pepper. |
| Institute | Osaka University |
| Department | Biotechnology |
| Laboratory | Bioresource Engineering |
| Last Name | Shimma |
| First Name | Shuichi |
| Address | Yamadaoka 2-1, Suita, Osaka, Japan |
| sshimma@bio.eng.osaka-u.ac.jp | |
| Phone | +8106-6879-4170 |
| Submit Date | 2022-03-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | lcd |
| Analysis Type Detail | MALDI-MS |
| Release Date | 2022-07-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001358 |
| Project DOI: | doi: 10.21228/M85X2H |
| Project Title: | Structure-based non-targeted mass spectrometry imaging analysis of dried long pepper |
| Project Type: | Non-targeted mass spectrometry imaging of dried long pepper |
| Project Summary: | The attached MSI datasets of dried long pepper sections were analyzed in the manuscript by Wisman et al. Below is taken from the abstract: Many studies have applied multivariate analysis to mass spectrometry imaging (MSI) data to differentiate structures in a sample based on information of compound spatial distribution. However, the actual distribution of individual compounds remains poorly documented, especially in plant samples. Here, a detailed non-targeted approach for plant sample analysis applied for the study of compound distribution in the medicinal species, long pepper, which has complicated internal structure. Principal component analysis (PCA) discriminated between the seed and pericarp regions, resulting in peaks that were closely associated with each region. Investigation of these peaks revealed slight but potentially important distribution differences between them, despite sharing the same accumulation profile in a region. Furthermore, piperamides such as piperine and piperlonguminine were among the compounds closely associated with the pericarp region. This is the first study to report the utilization of PCA for spatial feature extraction and the localization of piperamides in dried long pepper. |
| Institute: | Osaka University |
| Department: | Biotechnology |
| Laboratory: | Bioresource Engineering |
| Last Name: | Shimma |
| First Name: | Shuichi |
| Address: | Yamadaoka 2-1, Suita, Osaka, Japan |
| Email: | sshimma@bio.eng.osaka-u.ac.jp |
| Phone: | +8106-6879-4170 |
Subject:
| Subject ID: | SU002229 |
| Subject Type: | Plant |
| Subject Species: | Piper longum |
| Taxonomy ID: | 49511 |
Factors:
Subject type: Plant; Subject species: Piper longum (Factor headings shown in green)
| mb_sample_id | local_sample_id | Analysis Type |
|---|---|---|
| SA205773 | LP013 | MS/MS |
| SA205774 | LP012 | MS/MS |
| SA205775 | LP011 | MS/MS |
| SA205776 | M01 | Non-targeted |
| SA205777 | LP010 | Non-targeted |
| Showing results 1 to 5 of 5 |
Collection:
| Collection ID: | CO002222 |
| Collection Summary: | As stated in the manuscript: Dried fruits of P. longum were obtained from S&B Foods and kept in a dry container at around 25 °C. |
| Sample Type: | Dried long pepper |
| Storage Conditions: | Room temperature |
Treatment:
| Treatment ID: | TR002241 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP002235 |
| Sampleprep Summary: | As stated in the manuscript: A dried long pepper was appropriately cut to small pieces before embedding horizontally in 4% carboxymethyl cellulose and freezing at −80 °C. The frozen block was then attached to a sample holder with an optimal cutting temperature compound for sectioning inside a cryostat at −20 °C. Longitudinal sections 20 µm thick were collected using an adhesive cryofilm (type II C(10), SECTION-LAB, Hiroshima, Japan). The collected sections were attached using conductive double-sided tape on an indium tin oxide (ITO) glass-slide. The CHCA matrix was deposited at a thickness of 0.7 μm using a vacuum deposition system (iMLayer; Shimadzu, Kyoto, Japan). Recrystallization was performed by exposing the matrix-coated section to 5% methanol vapor at 60 °C, for 30 s. |
Combined analysis:
| Analysis ID | AN003509 | AN003510 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Unspecified | Unspecified |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | MALDI | MALDI |
| MS instrument type | MALDI IT-TOF | MALDI IT-TOF |
| MS instrument name | Shimadzu iMScope TRIO | Shimadzu iMScope TRIO |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Da | Da |
Chromatography:
| Chromatography ID: | CH002591 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS003267 |
| Analysis ID: | AN003509 |
| Instrument Name: | Shimadzu iMScope TRIO |
| Instrument Type: | MALDI IT-TOF |
| MS Type: | MALDI |
| MS Comments: | Shimadzu IMAGEREVEAL software |
| Ion Mode: | POSITIVE |
| Matrix: | CHCA |
| Analysis Protocol File: | lp_scan.pdf |
| MS ID: | MS003268 |
| Analysis ID: | AN003510 |
| Instrument Name: | Shimadzu iMScope TRIO |
| Instrument Type: | MALDI IT-TOF |
| MS Type: | MALDI |
| MS Comments: | Shimadzu IMAGEREVEAL software |
| Ion Mode: | POSITIVE |
| Collision Energy: | 70 |
| Matrix: | CHCA |
| Analysis Protocol File: | lp_ms2.pdf |
