Summary of Study ST002212

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001413. The data can be accessed directly via it's Project DOI: 10.21228/M82X3Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002212
Study TitleHuman fecal metabolome profiles under 3 different dietary terms
Study SummaryThe gut microbiota produce numerous metabolites that affect host physiology, although the effects of the daily diet on human intestinal metabolome profiles and robustness are not well understood. Here we investigated the robustness of the human intestinal environment through gut microbiome and metabolome profiles in response to daily dietary fluctuations. We analyzed 176 fecal samples from 25 healthy Japanese individuals under three dietary conditions, including heterogeneous and homogeneous diets. Human intestinal metabolome and microbiome profiles were unique to each individual and were robust under daily dietary fluctuation in most cases. Our findings provide insight into the use of intestinal environment information for clinical studies.
Institute
Keio University
DepartmentInstitute for Advanced Biosciences
Last NameFukuda
First NameShinji
AddressKakuganji 246-2, Mizukami, Tsuruoka City Yamagata,Japan
Emailsfukuda@sfc.keio.ac.jp
Phone+81-235-29-800
Submit Date2022-06-29
Raw Data AvailableYes
Raw Data File Type(s)OTHER
Analysis Type DetailLC-MS
Release Date2023-07-28
Release Version1
Shinji Fukuda Shinji Fukuda
https://dx.doi.org/10.21228/M82X3Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001413
Project DOI:doi: 10.21228/M82X3Z
Project Title:Human fecal metabolome profiles under 3 different dietary terms
Project Summary:The gut microbiota produce numerous metabolites that affect host physiology, although the effects of the daily diet on human intestinal metabolome profiles and robustness are not well understood. Here we investigated the robustness of the human intestinal environment through gut microbiome and metabolome profiles in response to daily dietary fluctuations. We analyzed 176 fecal samples from 25 healthy Japanese individuals under three dietary conditions, including heterogeneous and homogeneous diets. Human intestinal metabolome and microbiome profiles were unique to each individual and were robust under daily dietary fluctuation in most cases. Our findings provide insight into the use of intestinal environment information for clinical studies.
Institute:Keio University
Department:Institute for Advanced Biosciences
Last Name:Fukuda
First Name:Shinji
Address:Kakuganji 246-2, Mizukami, Tsuruoka City Yamagata,Japan
Email:sfukuda@sfc.keio.ac.jp
Phone:+81-235-29-800

Subject:

Subject ID:SU002298
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diet_phase Timepoint
SA21165610A1A(Term1) A1
SA21165724A1A(Term1) A1
SA21165821A1A(Term1) A1
SA21165904A2A(Term1) A2
SA21166020A2A(Term1) A2
SA21166118A2A(Term1) A2
SA21166223A2A(Term1) A2
SA21166324A2A(Term1) A2
SA21166432A2A(Term1) A2
SA21166514A2A(Term1) A2
SA21166608A2A(Term1) A2
SA21166730A2A(Term1) A2
SA21166804A3A(Term1) A3
SA21166921A3A(Term1) A3
SA21167032A3A(Term1) A3
SA21167130A3A(Term1) A3
SA21167220A3A(Term1) A3
SA21167314A3A(Term1) A3
SA21167415A3A(Term1) A3
SA21167502A3A(Term1) A3
SA21167610A4A(Term1) A4
SA21167709A4A(Term1) A4
SA21167814A4A(Term1) A4
SA21167922A4A(Term1) A4
SA21168011A4A(Term1) A4
SA21168127A4A(Term1) A4
SA21168205A4A(Term1) A4
SA21168324A4A(Term1) A4
SA21168401A5A(Term1) A5
SA21168522A5A(Term1) A5
SA21168614A5A(Term1) A5
SA21168716A5A(Term1) A5
SA21168832A5A(Term1) A5
SA21168930A5A(Term1) A5
SA21169020A5-1A(Term1) A5-1
SA21169120A5-2A(Term1) A5-2
SA21169210A6A(Term1) A6
SA21169316A6A(Term1) A6
SA21169402A6A(Term1) A6
SA21169501A6A(Term1) A6
SA21169628A6A(Term1) A6
SA21169714A6A(Term1) A6
SA21169826A6A(Term1) A6
SA21169928A7A(Term1) A7
SA21170015A7A(Term1) A7
SA21170104A7A(Term1) A7
SA21170224A7A(Term1) A7
SA21170307A7A(Term1) A7
SA21170406A7A(Term1) A7
SA21170520A7A(Term1) A7
SA21170601A7A(Term1) A7
SA21170703A7A(Term1) A7
SA21170830A7A(Term1) A7
SA21170911A7A(Term1) A7
SA21171016B1B(Term2) B1
SA21171101B1B(Term2) B1
SA21171210B1B(Term2) B1
SA21171323B1B(Term2) B1
SA21171418B1B(Term2) B1
SA21171522B1B(Term2) B1
SA21171624B1B(Term2) B1
SA21171732B1B(Term2) B1
SA21171830B1B(Term2) B1
SA21171914B1B(Term2) B1
SA21172021B2B(Term2) B2
SA21172124B2B(Term2) B2
SA21172214B2B(Term2) B2
SA21172320B2B(Term2) B2
SA21172411B2B(Term2) B2
SA21172505B2B(Term2) B2
SA21172610B2B(Term2) B2
SA21172704B2B(Term2) B2
SA21172828B2B(Term2) B2
SA21172932B2B(Term2) B2
SA21173016B3B(Term2) B3
SA21173115B3B(Term2) B3
SA21173208B3B(Term2) B3
SA21173314B3B(Term2) B3
SA21173422B3B(Term2) B3
SA21173506B4B(Term2) B4
SA21173609B4B(Term2) B4
SA21173720B4B(Term2) B4
SA21173807B4B(Term2) B4
SA21173924B4B(Term2) B4
SA21174028B4B(Term2) B4
SA21174122B4B(Term2) B4
SA21174214B4B(Term2) B4
SA21174304B4B(Term2) B4
SA21174410B4B(Term2) B4
SA21174527B5B(Term2) B5
SA21174632B5B(Term2) B5
SA21174730B5B(Term2) B5
SA21174826B5B(Term2) B5
SA21174914B5B(Term2) B5
SA21175011B5B(Term2) B5
SA21175101B5B(Term2) B5
SA21175208B5B(Term2) B5
SA21175304B6B(Term2) B6
SA21175421B6B(Term2) B6
SA21175501B6B(Term2) B6
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Collection:

Collection ID:CO002291
Collection Summary:Human fecal metabolome profiles under 3 different dietary terms. During the first phase (term 1), there was no restriction on diet, but consumption of medicinal drugs and dietary supplements were prohibited. In the second phase (term 2), subjects ate provided meals. The menus of meals were different every time. For the last phase (term 3), subjects continuously ate an identical meal 15 times, three times a day over 5 days. Commercially available box meals, frozen foods, fruits, sandwiches, rice balls, retort foods were provided in term 2 and 3. Throughout the trial, subjects collected their feces and froze them as soon as they could. After which, the frozen fecal samples were shipped frozen to the Institute for Advanced Biosciences, Keio University, Yamagata, Japan, for metabolome and metagenome analysis.
Sample Type:Feces

Treatment:

Treatment ID:TR002310
Treatment Summary:Human fecal metabolome profiles under 3 different dietary terms. During the first phase (term 1), there was no restriction on diet, but consumption of medicinal drugs and dietary supplements were prohibited. In the second phase (term 2), subjects ate provided meals. The menus of meals were different every time. For the last phase (term 3), subjects continuously ate an identical meal 15 times, three times a day over 5 days. Commercially available box meals, frozen foods, fruits, sandwiches, rice balls, retort foods were provided in term 2 and 3. Throughout the trial, subjects collected their feces and froze them as soon as they could. After which, the frozen fecal samples were shipped frozen to the Institute for Advanced Biosciences, Keio University, Yamagata, Japan, for metabolome and metagenome analysis.

Sample Preparation:

Sampleprep ID:SP002304
Sampleprep Summary:Fecal samples were freeze-dried, and then metabolites were extracted using the methanol:chloroform:water extraction protocol.

Combined analysis:

Analysis ID AN003616 AN003617
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent CE Agilent CE
Column Fused-silica,50um(cation) COSMO(+),50um(anion
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Ion Mode POSITIVE NEGATIVE
Units μmol/g μmol/g

Chromatography:

Chromatography ID:CH002671
Instrument Name:Agilent CE
Column Name:Fused-silica,50um(cation)
Internal Standard:Methionine sulfone
Chromatography Type:CE
  
Chromatography ID:CH002672
Instrument Name:Agilent CE
Column Name:COSMO(+),50um(anion
Internal Standard:D-camphor-10-sulfonic acid
Chromatography Type:CE

MS:

MS ID:MS003367
Analysis ID:AN003616
Instrument Name:Agilent CE-TOFMS
Instrument Type:TOF
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:POSITIVE
  
MS ID:MS003368
Analysis ID:AN003617
Instrument Name:Agilent CE-TOFMS
Instrument Type:TOF
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:NEGATIVE
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