Summary of Study ST002254

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001442. The data can be accessed directly via it's Project DOI: 10.21228/M8B71S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002254
Study TitleMaternal obesity alters offspring liver and skeletal muscle metabolism in early post-puberty despite maintaining a normal post-weaning dietary lifestyle
Study SummaryMaternal obesity (MO) during pregnancy is linked to increased and premature risk of age-related metabolic diseases in the offspring. However, the underlying molecular mechanisms still remain not fully understood. Using a well-established baboon model of MO, we analyzed tissue biopsies and plasma samples obtained from post-pubertal offspring (3-6.5y at sample collection) of MO mothers (n=19) and from control animals born to mothers fed a standard diet (CON, n=13). All offspring ate normal chow diet after weaning. With an untargeted gas chromatography-mass spectrometry metabolomics profiling, we quantified a total of 351 liver, 316 skeletal muscle and 423 plasma metabolites. We found 58 metabolites significantly altered in liver and 46 in skeletal muscle of MO offspring, including 8 metabolites shared between both tissues. Male and female-specific metabolites in opposite direction of change were found in liver and skeletal muscle of MO offspring. Several tissue-specific and 4 shared metabolic pathways were identified from these dysregulated metabolites. Interestingly, none of the tissue-specific metabolic alterations reflected in plasma. Our results identify tissue metabolites and pathways in post-pubertal MO offspring in a sex-specific manner.
Institute
Wake Forest School of Medicine
Last NameAmpong
First NameIsaac
AddressCenter for Precision Medicine, Department of Internal Medicine, Section on Molecular Medicine, Wake Forest University, Winston-Salem, North Carolina, United States
Emailiampong@wakehealth.edu
Phone3367162091
Submit Date2022-08-02
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC-MS
Release Date2022-08-31
Release Version1
Isaac Ampong Isaac Ampong
https://dx.doi.org/10.21228/M8B71S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001442
Project DOI:doi: 10.21228/M8B71S
Project Title:Maternal obesity alters offspring liver and skeletal muscle metabolism in early post-puberty despite maintaining a normal post-weaning dietary lifestyle
Project Summary:Maternal obesity (MO) during pregnancy is linked to increased and premature risk of age-related metabolic diseases in the offspring. However, the underlying molecular mechanisms still remain not fully understood. Using a well-established baboon model of MO, we analyzed tissue biopsies and plasma samples obtained from post-pubertal offspring (3-6.5y at sample collection) of MO mothers (n=19) and from control animals born to mothers fed a standard diet (CON, n=13). All offspring ate normal chow diet after weaning. With an untargeted gas chromatography-mass spectrometry metabolomics profiling, we quantified a total of 351 liver, 316 skeletal muscle and 423 plasma metabolites. We found 58 metabolites significantly altered in liver and 46 in skeletal muscle of MO offspring, including 8 metabolites shared between both tissues. Male and female-specific metabolites in opposite direction of change were found in liver and skeletal muscle of MO offspring. Several tissue-specific and 4 shared metabolic pathways were identified from these dysregulated metabolites. Interestingly, none of the tissue-specific metabolic alterations reflected in plasma. Our results identify tissue metabolites and pathways in post-pubertal MO offspring in a sex-specific manner.
Institute:Wake Forest School of Medicine
Department:Department of Internal Medicine
Laboratory:Olivier Lab
Last Name:Ampong
First Name:Isaac
Address:Center for Precision Medicine, Department of Internal Medicine, Section on Molecular Medicine, Wake Forest University, Winston-Salem, North Carolina, United States
Email:iampong@wakehealth.edu
Phone:3367162091

Subject:

Subject ID:SU002340
Subject Type:Mammal
Subject Species:Papio hamadryas
Taxonomy ID:9557

Factors:

Subject type: Mammal; Subject species: Papio hamadryas (Factor headings shown in green)

mb_sample_id local_sample_id Sample type
SA216995L34776LIVER
SA216996L34832LIVER
SA216997L34210LIVER
SA216998L34163LIVER
SA216999L34155LIVER
SA217000L34867LIVER
SA217001L34165LIVER
SA217002L34913LIVER
SA217003L35315LIVER
SA217004L36492LIVER
SA217005L34984LIVER
SA217006L34953LIVER
SA217007L33895LIVER
SA217008L34952LIVER
SA217009L32650LIVER
SA217010L34906LIVER
SA217011L32927LIVER
SA217012L34156LIVER
SA217013L34160LIVER
SA217014L32862LIVER
SA217015L32798LIVER
SA217016L33893LIVER
SA217017L32771LIVER
SA217018L34172LIVER
SA217019L34166LIVER
SA217020L33857LIVER
SA217021L33873LIVER
SA217022L33601LIVER
SA217023L33472LIVER
SA217024L34777LIVER
SA217025L32732LIVER
SA217026L33308LIVER
SA217027LQC22NIST PLASMA
SA217028LQC11NIST PLASMA
SA217029LQC33NIST PLASMA
SA217030LQC66NIST PLASMA
SA217031LQC44NIST PLASMA
SA217032LQC55NIST PLASMA
SA217033P34832PLASMA
SA217034P34867PLASMA
SA217035P34776PLASMA
SA217036P34210PLASMA
SA217037P34906PLASMA
SA217038P34777PLASMA
SA217039P34952PLASMA
SA217040P36492PLASMA
SA217041P35315PLASMA
SA217042P34984PLASMA
SA217043P34953PLASMA
SA217044P34913PLASMA
SA217045P34172PLASMA
SA217046P32732PLASMA
SA217047P32650PLASMA
SA217048P33472PLASMA
SA217049P32771PLASMA
SA217050P32862PLASMA
SA217051P34166PLASMA
SA217052P33308PLASMA
SA217053P32927PLASMA
SA217054P33601PLASMA
SA217055P32798PLASMA
SA217056P34160PLASMA
SA217057P34163PLASMA
SA217058P33857PLASMA
SA217059P34165PLASMA
SA217060P34156PLASMA
SA217061P33873PLASMA
SA217062P33893PLASMA
SA217063P33895PLASMA
SA217064P34155PLASMA
SA21706534906SKELETAL MUSCLE
SA21706634867SKELETAL MUSCLE
SA21706734777SKELETAL MUSCLE
SA21706834776SKELETAL MUSCLE
SA21706934832SKELETAL MUSCLE
SA21707035315SKELETAL MUSCLE
SA21707136492SKELETAL MUSCLE
SA21707234210SKELETAL MUSCLE
SA21707334984SKELETAL MUSCLE
SA21707434953SKELETAL MUSCLE
SA21707534952SKELETAL MUSCLE
SA21707634913SKELETAL MUSCLE
SA21707734155SKELETAL MUSCLE
SA21707832927SKELETAL MUSCLE
SA21707933308SKELETAL MUSCLE
SA21708033472SKELETAL MUSCLE
SA21708132862SKELETAL MUSCLE
SA21708232798SKELETAL MUSCLE
SA21708332650SKELETAL MUSCLE
SA21708432732SKELETAL MUSCLE
SA21708532771SKELETAL MUSCLE
SA21708633601SKELETAL MUSCLE
SA21708733857SKELETAL MUSCLE
SA21708834163SKELETAL MUSCLE
SA21708934165SKELETAL MUSCLE
SA21709034166SKELETAL MUSCLE
SA21709134160SKELETAL MUSCLE
SA21709234156SKELETAL MUSCLE
SA21709333873SKELETAL MUSCLE
SA21709433893SKELETAL MUSCLE
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Collection:

Collection ID:CO002333
Collection Summary:The liver, skeletal muscle and plasma samples consisted of 32 samples each which were obtained from post-pubertal offspring (3-6.5y at sample collection) of MO mothers (n=19) and from control animals born to mothers fed a standard diet (CON, n=13). The datasets were generated from metabolic profiling of these tissues and plasma samples collected from 17 females in the age range of 3-6.5 years and 13 males in the same age range. All were analyzed using an untargeted EI-GC-MS approach as described above.
Sample Type:Liver

Treatment:

Treatment ID:TR002352
Treatment Summary:We analyzed liver and muscle biopsies and plasma samples obtained from the post-pubertal offspring (3-6.5 years old at sample collection) of MO mothers (n=19, 10 females and 9 males) and from control animals born to mothers fed a standard diet (n=13, 6 females and 7 males). All offspring animals ate normal chow diet after weaning so differences between groups are due to the impact of the maternal intrauterine environment.

Sample Preparation:

Sampleprep ID:SP002346
Sampleprep Summary:15 μL of plasma, liver or skeletal muscle samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA.

Combined analysis:

Analysis ID AN003682
Analysis type MS
Chromatography type GC
Chromatography system Thermo Trace 1310
Column Thermo Scientific Trace GOLD TG-5SIL-MS
MS Type EI
MS instrument type QTRAP
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units Normalized Peak Intensities

Chromatography:

Chromatography ID:CH002730
Instrument Name:Thermo Trace 1310
Column Name:Thermo Scientific Trace GOLD TG-5SIL-MS
Chromatography Type:GC

MS:

MS ID:MS003433
Analysis ID:AN003682
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:QTRAP
MS Type:EI
MS Comments:Data acquisition and instrument control were carried out using Xcalibur 4.3 and Trace-Finder 4.1 softwares MS-DIAL
Ion Mode:POSITIVE
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