Summary of Study ST002262

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001443. The data can be accessed directly via it's Project DOI: 10.21228/M86H66 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002262
Study Title	Metabolomics and metagenomics of pediatric obesity (Feces)
Study Summary	Pediatric obesity has grown as important global health problem in the world. The pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. However, the mechanism of both the pediatric obesity and treatment of the obesity remains unclear. Therefore, we investigated metabolomic pathways related to the pediatric obesity and the treatment through metabolomics and metagenomics approaches.
Institute	Seoul National University College of Medicine and Hospital
Department	Department of Clinical Pharmacology and Therapeutics
Last Name	Lee
First Name	Yujin
Address	101 Daehak-ro, Jongno-gu, Seoul 110-799, Korea, Seoul, Seoul, 03080, Korea, South
Email	yoojinlee@snu.ac.kr
Phone	+82-10-3380-4686
Submit Date	2022-07-10
Raw Data Available	Yes
Raw Data File Type(s)	smp
Analysis Type Detail	GC-MS
Release Date	2023-01-04
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001443
Project DOI:	doi: 10.21228/M86H66
Project Title:	Mechanistic study of pediatric obesity through metabolomics and metagenomics
Project Summary:	Pediatric obesity has grown as an important global health problem in the world. Pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. Although many researchers reported results about the pediatric obesity study, the mechanism of both pediatric obesity and its treatment remains unclear. Therefore, we investigated the metabolomic pathways related to pediatric obesity and the treatment through metabolomics and metagenomics approaches.
Institute:	Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine and Hospital
Last Name:	Lee
First Name:	Yujin
Address:	101 Daehak-ro, Jongno-gu, Seoul 110-799, Korea, Seoul, Seoul, 03080, Korea, South
Email:	yoojinlee@snu.ac.kr
Phone:	+82-10-3380-4686

Subject:

Subject ID:	SU002348
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Gender	Group
SA217332	N10	F	Normal
SA217333	N12	F	Normal
SA217334	N14	F	Normal
SA217335	N08	F	Normal
SA217336	N19	F	Normal
SA217337	N04	F	Normal
SA217338	N22	F	Normal
SA217339	M10	F	Obe
SA217340	M11	F	Obe
SA217341	M06	F	Obe
SA217342	M07	F	Obe
SA217343	M09	F	Obe
SA217344	M14	F	Obe
SA217345	M19	F	Obe
SA217346	M32	F	Obe
SA217347	M41	F	Obe
SA217348	M31	F	Obe
SA217349	M29	F	Obe
SA217350	M02	F	Obe
SA217351	M25	F	Obe
SA217352	M16	F	Obe
SA217353	M08	F	Obe
SA217354	N11	M	Normal
SA217355	N13	M	Normal
SA217356	N16	M	Normal
SA217357	N05	M	Normal
SA217358	N06	M	Normal
SA217359	N09	M	Normal
SA217360	N07	M	Normal
SA217361	N17	M	Normal
SA217362	N15	M	Normal
SA217363	N01	M	Normal
SA217364	N03	M	Normal
SA217365	N21	M	Normal
SA217366	N20	M	Normal
SA217367	N02	M	Normal
SA217368	N18	M	Normal
SA217369	M42	M	Obe
SA217370	M28	M	Obe
SA217371	M35	M	Obe
SA217372	M40	M	Obe
SA217373	M26	M	Obe
SA217374	M37	M	Obe
SA217375	M36	M	Obe
SA217376	M33	M	Obe
SA217377	M20	M	Obe
SA217378	M12	M	Obe
SA217379	M15	M	Obe
SA217380	M05	M	Obe
SA217381	M04	M	Obe
SA217382	M03	M	Obe
SA217383	M17	M	Obe
SA217384	M18	M	Obe
SA217385	M23	M	Obe
SA217386	M22	M	Obe
SA217387	M21	M	Obe
SA217388	M01	M	Obe
SA217389	M24	M	Obe

Showing results 1 to 58 of 58

Showing results 1 to 58 of 58

Collection:

Collection ID:	CO002341
Collection Summary:	This longitudinal cohort study is an analysis of fecal samples collected from obese children before and after a 2-month weight reduction program that consisted of three visits to the hospital.
Sample Type:	Feces

Treatment:

Treatment ID:	TR002360
Treatment Summary:	Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit.

Treatment ID:

TR002360

Treatment Summary:

Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit.

Sample Preparation:

Sampleprep ID:	SP002354
Sampleprep Summary:	Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Sampleprep ID:

SP002354

Sampleprep Summary:

Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Combined analysis:

Analysis ID	AN003695
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890B
Column	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type	EI
MS instrument type	GC-TOF
MS instrument name	Leco Pegasus HT TOF
Ion Mode	NEGATIVE
Units	intensity

Chromatography:

Chromatography ID:	CH002738
Instrument Name:	Agilent 7890B
Column Name:	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:	GC

MS:

MS ID:	MS003446
Analysis ID:	AN003695
Instrument Name:	Leco Pegasus HT TOF
Instrument Type:	GC-TOF
MS Type:	EI
MS Comments:	The LECO Corporation ChromaTOF®-GC software (v4.72) was used for data acquisition and extraction. This included computation of baseline (offset of 1; above the noise) and automatic entering the number of data points for smoothing. The software's Statistical Compare was used to align peaks. The MS spectral matching was performed using the NIST and Wiley9 libraries. Also, retention index method was used for identification.
Ion Mode:	NEGATIVE