Summary of Study ST002299

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001473. The data can be accessed directly via it's Project DOI: 10.21228/M8B40C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002299
Study TitleCharacterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Healthy Start Cohort)-Part 1
Study TypeProspective cohort study
Study SummaryHealthy Start is a prospective, pre-birth cohort study that recruited pregnant participants from outpatient prenatal clinics at the University of Colorado Hospital between 2009 and 2014. Eligible participants were 16 years or older with singleton pregnancies, no history of stillbirth or extremely preterm birth (<25 weeks of gestation) and no serious medical conditions, and had not yet completed 24 weeks of gestation at the time of enrollment. Mothers completed two study visits during pregnancy (median gestational ages 17 and 27 weeks). Mother-child pairs were assessed at birth for neonatal outcomes, and are currently still being followed through 8-10 years postpartum (NB: outcomes for this proposal are at birth). For the present project, we will use data from 1,297 mother-child pairs with adequate maternal plasma at 17 and 27 gestational weeks for untargeted metabolomics profiling. Please contact Wei Perng at wei.perng@cuanschutz.edu for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
University of Colorado Anschutz Medical Campus
DepartmentEpidemiology; Lifecourse Epidemiology of Adiposity and Diabetes (LEAD) Center
Last NamePerng
First NameWei
Address1890 North Revere Court, Campus Box F426, Rm 1014, Aurora, CO 80010
EmailWei.Perng@CUAnschutz.edu
Phone734-717-0982
Submit Date2022-09-08
Total Subjects2123
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2023-10-02
Release Version1
Wei Perng Wei Perng
https://dx.doi.org/10.21228/M8B40C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001473
Project DOI:doi: 10.21228/M8B40C
Project Title:Characterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Healthy Start Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project Viva, Atlanta ECHO, Paneth Cohort, and PETALS. This project will generate new untargeted metabolomics profiling data at two time-points in pregnancy for all women in order to identify trimester-specific metabolomics signatures and longitudinal metabolomics trajectories associated with offspring birth size; identify the contribution of maternal sociodemographic and perinatal characteristics to the metabolomics signatures.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:9843770693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002385
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA221056PZDa_HHEAR_152HHEAR Pool
SA221057PZDa_HHEAR_151HHEAR Pool
SA221058PZDa_HHEAR_153HHEAR Pool
SA221059PZDa_HHEAR_154HHEAR Pool
SA221060PZDa_HHEAR_155HHEAR Pool
SA221061PZDa_HHEAR_150HHEAR Pool
SA221062PZDa_HHEAR_148HHEAR Pool
SA221063PZDa_HHEAR_145HHEAR Pool
SA221064PZDa_HHEAR_144HHEAR Pool
SA221065PZDa_HHEAR_146HHEAR Pool
SA221066PZDa_HHEAR_147HHEAR Pool
SA221067PZDa_HHEAR_156HHEAR Pool
SA221068PZDa_HHEAR_149HHEAR Pool
SA221069PZDa_HHEAR_157HHEAR Pool
SA221070PZDa_HHEAR_165HHEAR Pool
SA221071PZDa_HHEAR_164HHEAR Pool
SA221072PZDa_HHEAR_166HHEAR Pool
SA221073PZDa_HHEAR_167HHEAR Pool
SA221074PZDa_HHEAR_168HHEAR Pool
SA221075PZDa_HHEAR_163HHEAR Pool
SA221076PZDa_HHEAR_162HHEAR Pool
SA221077PZDa_HHEAR_158HHEAR Pool
SA221078PZDa_HHEAR_159HHEAR Pool
SA221079PZDa_HHEAR_160HHEAR Pool
SA221080PZDa_HHEAR_161HHEAR Pool
SA221081PZDa_HHEAR_143HHEAR Pool
SA221082PZDa_HHEAR_141HHEAR Pool
SA221083PZDa_HHEAR_125HHEAR Pool
SA221084PZDa_HHEAR_124HHEAR Pool
SA221085PZDa_HHEAR_126HHEAR Pool
SA221086PZDa_HHEAR_127HHEAR Pool
SA221087PZDa_HHEAR_128HHEAR Pool
SA221088PZDa_HHEAR_123HHEAR Pool
SA221089PZDa_HHEAR_122HHEAR Pool
SA221090PZDa_HHEAR_118HHEAR Pool
SA221091PZDa_HHEAR_117HHEAR Pool
SA221092PZDa_HHEAR_119HHEAR Pool
SA221093PZDa_HHEAR_120HHEAR Pool
SA221094PZDa_HHEAR_121HHEAR Pool
SA221095PZDa_HHEAR_129HHEAR Pool
SA221096PZDa_HHEAR_130HHEAR Pool
SA221097PZDa_HHEAR_138HHEAR Pool
SA221098PZDa_HHEAR_137HHEAR Pool
SA221099PZDa_HHEAR_139HHEAR Pool
SA221100PZDa_HHEAR_140HHEAR Pool
SA221101PZDa_HHEAR_169HHEAR Pool
SA221102PZDa_HHEAR_136HHEAR Pool
SA221103PZDa_HHEAR_135HHEAR Pool
SA221104PZDa_HHEAR_131HHEAR Pool
SA221105PZDa_HHEAR_132HHEAR Pool
SA221106PZDa_HHEAR_133HHEAR Pool
SA221107PZDa_HHEAR_134HHEAR Pool
SA221108PZDa_HHEAR_142HHEAR Pool
SA221109PZDa_HHEAR_170HHEAR Pool
SA221110PZDa_HHEAR_205HHEAR Pool
SA221111PZDa_HHEAR_204HHEAR Pool
SA221112PZDa_HHEAR_206HHEAR Pool
SA221113PZDa_HHEAR_207HHEAR Pool
SA221114PZDa_HHEAR_208HHEAR Pool
SA221115PZDa_HHEAR_203HHEAR Pool
SA221116PZDa_HHEAR_202HHEAR Pool
SA221117PZDa_HHEAR_198HHEAR Pool
SA221118PZDa_HHEAR_197HHEAR Pool
SA221119PZDa_HHEAR_199HHEAR Pool
SA221120PZDa_HHEAR_200HHEAR Pool
SA221121PZDa_HHEAR_201HHEAR Pool
SA221122PZDa_HHEAR_210HHEAR Pool
SA221123PZDa_HHEAR_211HHEAR Pool
SA221124PZDa_HHEAR_219HHEAR Pool
SA221125PZDa_HHEAR_218HHEAR Pool
SA221126PZDa_HHEAR_220HHEAR Pool
SA221127PZDa_HHEAR_221HHEAR Pool
SA221128PZDa_HHEAR_9HHEAR Pool
SA221129PZDa_HHEAR_217HHEAR Pool
SA221130PZDa_HHEAR_216HHEAR Pool
SA221131PZDa_HHEAR_212HHEAR Pool
SA221132PZDa_HHEAR_213HHEAR Pool
SA221133PZDa_HHEAR_214HHEAR Pool
SA221134PZDa_HHEAR_215HHEAR Pool
SA221135PZDa_HHEAR_196HHEAR Pool
SA221136PZDa_HHEAR_195HHEAR Pool
SA221137PZDa_HHEAR_178HHEAR Pool
SA221138PZDa_HHEAR_177HHEAR Pool
SA221139PZDa_HHEAR_179HHEAR Pool
SA221140PZDa_HHEAR_180HHEAR Pool
SA221141PZDa_HHEAR_181HHEAR Pool
SA221142PZDa_HHEAR_176HHEAR Pool
SA221143PZDa_HHEAR_175HHEAR Pool
SA221144PZDa_HHEAR_171HHEAR Pool
SA221145PZDa_HHEAR_172HHEAR Pool
SA221146PZDa_HHEAR_173HHEAR Pool
SA221147PZDa_HHEAR_174HHEAR Pool
SA221148PZDa_HHEAR_182HHEAR Pool
SA221149PZDa_HHEAR_183HHEAR Pool
SA221150PZDa_HHEAR_191HHEAR Pool
SA221151PZDa_HHEAR_190HHEAR Pool
SA221152PZDa_HHEAR_192HHEAR Pool
SA221153PZDa_HHEAR_193HHEAR Pool
SA221154PZDa_HHEAR_194HHEAR Pool
SA221155PZDa_HHEAR_189HHEAR Pool
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Collection:

Collection ID:CO002378
Collection Summary:Healthy Start is a prospective, pre-birth cohort study that recruited pregnant participants from outpatient prenatal clinics at the University of Colorado Hospital between 2009 and 2014. Eligible participants were 16 years or older with singleton pregnancies, no history of stillbirth or extremely preterm birth (<25 weeks of gestation) and no serious medical conditions, and had not yet completed 24 weeks of gestation at the time of enrollment. Mothers completed two study visits during pregnancy (median gestational ages 17 and 27 weeks). Mother-child pairs were assessed at birth for neonatal outcomes, and are currently still being followed through 8-10 years postpartum (NB: outcomes for this proposal are at birth). For the present project, we will use data from 1,297 mother-child pairs with adequate maternal plasma at 17 and 27 gestational weeks for untargeted metabolomics profiling.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002397
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002391
Sampleprep Summary:The LEAD Center of the University of Colorado Denver Anschutz Medical Campus aliquoted the study samples (50 µL) and made the total study pool sample according to the statement of work provided by UNC. These samples were shipped to the NC HHEAR Hub on dry ice. Before sample preparation, the NC HHEAR Hub thawed and mixed the total study pool samples and distributed them with 50 µL per aliquot, which were used as QC study pools throughout the whole study. HHEAR reference plasma (50 µL each) and NIST serum (909C) reference material (50 µL each) were provided by the NC HHEAR Hub. The LC-MS grade water (50 µL) was used as blank. Sample preparation was conducted by batch and all samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference serum, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm in room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN003755
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific™ Vanquish™ UPHPLC
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Normalized intensity

Chromatography:

Chromatography ID:CH002778
Chromatography Summary:Reverse phase
Instrument Name:Thermo Scientific™ Vanquish™ UPHPLC
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003498
Analysis ID:AN003755
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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