Summary of Study ST002311

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001482. The data can be accessed directly via it's Project DOI: 10.21228/M8598K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002311
Study TitleCecal Microbiota in Phage-treated Salmonella-infected broilers
Study SummaryAnalysis of the gastrointestinal microbiota of Salmonella-infected broilers has been carried out after the application of encapsulated bacteriophages in the feed, during the production cycle under farm conditions
Institute
Universidad Cardenal Herrera CEU
Last NameLorenzo-Rebenaque
First NameLaura
AddressEd. Seminario. S/n, Moncada, Valencia, 46113, Spain
Emaillaura.lorenzorebenaque@uchceu.es
Phone615056012
Submit Date2022-09-13
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-10-18
Release Version1
Laura Lorenzo-Rebenaque Laura Lorenzo-Rebenaque
https://dx.doi.org/10.21228/M8598K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001482
Project DOI:doi: 10.21228/M8598K
Project Title:Cecal Microbiota in Phage-treated Salmonella-infected broilers
Project Summary:Analysis of the gastrointestinal metabolome of Salmonella-infected broilers has been carried out after the application of encapsulated bacteriophages in the feed, during the production cycle under farm conditions
Institute:Universidad Cardenal Herrera CEU
Last Name:Lorenzo-Rebenaque
First Name:Laura
Address:Ed. Seminario. S/n, Moncada, Valencia, 46113, Spain
Email:laura.lorenzorebenaque@uchceu.es
Phone:615056012

Subject:

Subject ID:SU002397
Subject Type:Bird
Subject Species:Gallus gallus
Taxonomy ID:9031
Species Group:Birds

Factors:

Subject type: Bird; Subject species: Gallus gallus (Factor headings shown in green)

mb_sample_id local_sample_id Group Moment
SA22701733Phage Week 4
SA22701835Phage Week 4
SA22701936Phage Week 4
SA22702034Phage Week 4
SA22702143Phage Week 5
SA22702244Phage Week 5
SA22702342Phage Week 5
SA22702441Phage Week 5
SA22702550Phage Week 6
SA22702652Phage Week 6
SA22702751Phage Week 6
SA22702849Phage Week 6
SA22702930Salmonella Week 4
SA22703031Salmonella Week 4
SA22703132Salmonella Week 4
SA22703229Salmonella Week 4
SA22703339Salmonella Week 5
SA22703440Salmonella Week 5
SA22703538Salmonella Week 5
SA22703637Salmonella Week 5
SA22703745Salmonella Week 6
SA22703846Salmonella Week 6
SA22703947Salmonella Week 6
SA22704048Salmonella Week 6
Showing results 1 to 24 of 24

Collection:

Collection ID:CO002390
Collection Summary:Cecal sample were collected and immediately snap frozen for metabolomics analysis at the end of experiment
Sample Type:Cecum

Treatment:

Treatment ID:TR002409
Treatment Summary:Half of the animals in each experimental group were challenged with Salmonella Enteritidis (105 CFU/bird) at 24 h after placing. During the first 21 days of the chicken rearing period, Φ-treated group received 0.1% microencapsulated Salmonella-phage (108 PFU/g) encapsulated in Eudragit®L100 (a pH-responsive formulation) with the starter diet feed, and the control group did not receive any phage

Sample Preparation:

Sampleprep ID:SP002403
Sampleprep Summary:Cecal contents were lyophilized and homogenated. Then, 10 mg of the sample were mixed with 0.75mL of cold 75% (v/v) methanol, 0.1% (v/v) formic acid, spiked with 10μg/ml formononetin as internal standard. Then, the mix were shaked for 40’ at 20Hz using a Mixer Mill 300 (Qiagen) and centrifuged at 20,000 xg for 15 min at 4°C. The suspension (600μL) was transferred to a new 2-mL conical tube.

Chromatography:

Chromatography ID:CH002792
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Phenomenex Luna (100 x 2.1mm,2.5um)
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN003775
Analysis Type:MS
Chromatography ID:CH002792
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST002311_AN003775_Results.txt
Units:m/z ratio
  
Analysis ID:AN003776
Analysis Type:MS
Chromatography ID:CH002792
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST002311_AN003776_Results.txt
Units:m/z ratio
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