Summary of Study ST002335
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001498. The data can be accessed directly via it's Project DOI: 10.21228/M83D9C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002335 |
| Study Title | Pathogenic Auxilin mutations affect lipids that are critical to Synaptojanin function |
| Study Summary | Mass spectrometry was performed on heads of 15DO flies homogenized in 100 µl D-PBS (Dulbecco’s phosphate-buffered saline without Mg2+ and Ca2+) by Lipotype. Fifteen fly heads were pooled from three independent crosses for each analysis, and mass spectrometry was performed on n=7 for wild-type, n≥4 analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and dAuxRG/F956x and n=3 for dAuxWT/F956x and dAuxRG/F956x overexpressing Synj. |
| Institute | VIB-KU Leuven |
| Last Name | Jacquemyn |
| First Name | Julie |
| Address | Medical Sciences Building 7-25, Edmonton, Alberta, T6H 0L2, Canada |
| jacquemynjulie@ualberta.ca | |
| Phone | +1 (587) 3409325 |
| Submit Date | 2022-10-27 |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2022-11-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001498 |
| Project DOI: | doi: 10.21228/M83D9C |
| Project Title: | Parkinsonism mutations in DNAJC6 cause lipid defects and neurodegeneration that are rescued by Synj1 |
| Project Summary: | Recent evidence links dysfunctional lipid metabolism to the pathogenesis of Parkinson’s disease, but the mechanisms are not resolved. Here, we generated a new Drosophila knock-in model of DNAJC6/Auxilin and find that the pathogenic mutation causes synaptic dysfunction, neurological defects and neurodegeneration, as well as specific lipid metabolism alterations. In these mutants, membrane lipids containing long-chain polyunsaturated fatty acids, including phosphatidylinositol lipid species that are key for synaptic vesicle recycling and organelle function, are reduced. Overexpression of another protein mutated in Parkinson’s disease, Synaptojanin-1, known to bind and metabolize specific phosphoinositides, rescues the DNAJC6/Auxilin lipid alterations, the neuronal function defects and neurodegeneration. Our work reveals a functional relation between two proteins mutated in Parkinsonism and implicates deregulated phosphoinositide metabolism in the maintenance of neuronal integrity and neuronal survival. |
| Institute: | VIB-KU Leuven |
| Department: | Department of Neurosciences |
| Laboratory: | Laboratory of Neuronal Communication - Dr. Patrik Verstreken |
| Last Name: | Jacquemyn |
| First Name: | Julie |
| Address: | Medical Sciences Building 7-25, Edmonton, Alberta, T6H 0L2, Canada |
| Email: | jacquemynjulie@ualberta.ca |
| Phone: | 5873409325 |
Subject:
| Subject ID: | SU002423 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | 15 day old flies |
| Gender: | Male and female |
Factors:
Subject type: Invertebrate; Subject species: Drosophila melanogaster (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA232139 | S2 | AuWT/WT |
| SA232140 | S1 | AuWT/WT |
| SA232141 | S4 | AuWT/WT |
| SA232142 | S3 | AuWT/WT |
| SA232143 | S16 | AuxRG/F956x |
| SA232144 | S15 | AuxRG/F956x |
| SA232145 | S19 | AuxRG/F956x |
| SA232146 | S18 | AuxRG/F956x |
| SA232147 | S17 | AuxRG/F956x |
| SA232148 | S7 | AuxRG/RG |
| SA232149 | S6 | AuxRG/RG |
| SA232150 | S5 | AuxRG/RG |
| SA232151 | S9 | AuxRG/RG |
| SA232152 | S8 | AuxRG/RG |
| SA232153 | S12 | AuxWT/F956x |
| SA232154 | S11 | AuxWT/F956x |
| SA232155 | S10 | AuxWT/F956x |
| SA232156 | S13 | AuxWT/F956x |
| SA232157 | S14 | AuxWT/F956x |
| SA232158 | S23 | UAS-synj-AuxRG/F956x |
| SA232159 | S25 | UAS-synj-AuxRG/F956x |
| SA232160 | S24 | UAS-synj-AuxRG/F956x |
| SA232161 | S22 | UAS-synj-AuxWT/F956x |
| SA232162 | S21 | UAS-synj-AuxWT/F956x |
| SA232163 | S20 | UAS-synj-AuxWT/F956x |
| SA232164 | S31 | w1118 |
| SA232165 | S32 | w1118 |
| SA232166 | S30 | w1118 |
| SA232167 | S29 | w1118 |
| SA232168 | S26 | w1118 |
| SA232169 | S27 | w1118 |
| SA232170 | S28 | w1118 |
| Showing results 1 to 32 of 32 |
Collection:
| Collection ID: | CO002416 |
| Collection Summary: | Heads of 15DO flies were homogenized in 100 µl D-PBS (Dulbecco’s phosphate-buffered saline without Mg2+ and Ca2+) and stored at -80°C until processed for MS analysis by Lipotype. Fifteen fly heads were pooled from three independent crosses for each analysis, and mass spectrometry was performed on n=7 for wild-type, n≥4 analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and dAuxRG/F956x and n=3 for dAuxWT/F956x and dAuxRG/F956x overexpressing Synj. |
| Sample Type: | Insect tissue |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002435 |
| Treatment Summary: | In this study we did not apply any additional treatment(s) to the samples. |
Sample Preparation:
| Sampleprep ID: | SP002429 |
| Sampleprep Summary: | Lipids were extracted using chloroform and methanol (1). Prior to lipid extraction, samples were spiked with lipid class-specific internal standards. After drying and resuspending in an appropriate MS acquisition mixture, lipid extracts were infused directly in QExactive mass spectrometer (Thermo Fisher Scientific) with TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Sample preparations and analysis were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011). |
Combined analysis:
| Analysis ID | AN003814 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | pMol |
Chromatography:
| Chromatography ID: | CH002821 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS003556 |
| Analysis ID: | AN003814 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Acquired data was processed using lipid identification software based on LipidXplorer (1). Data post-processing and normalization were performed by Lipotype using a developed data management system. Data analysis was performed using GraphPad Prism software 9.3.1. Pmol values (obtained by MS) of individual lipid species were transformed into a fraction of the total PA, DAG, PI, PC, PE, and PS lipids in the sample. (1)Herzog, R. et al. Lipidxplorer: A software for consensual cross-platform lipidomics. PLoS ONE 7, e29851 (2012). |
| Ion Mode: | POSITIVE |
