Summary of Study ST002380

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001530. The data can be accessed directly via it's Project DOI: 10.21228/M8XH73 This work is supported by NIH grant, U2C- DK119886.

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Study IDST002380
Study TitleMetabolomic and Cultivation Insights into the Tolerance of the Spacecraft-Associated Acinetobacter Towards Kleenol 30, a Cleanroom Floor Detergent
Study Typeresearch
Study SummaryTo ensure cleanliness, NASA spacecraft are assembled in cleanroom facilities, where floors are routinely cleansed with Kleenol 30 (K30), an alkaline detergent. Through metabolomic and cultivation approaches, we show that cultures of spacecraft-associated Acinetobacter tolerate up to 1% v/v K30 and are fully inhibited at ≥2%; in comparison, NASA cleanrooms are cleansed with 0.8% K30. For A. johnsonii 2P08AA (isolated from a cleanroom floor), cultivations with 0.1% v/v K30 yield (1) limited changes in the intracellular metabolome and (2) increases in extracellular sugar acids, monosaccharides, organic acids, and fatty acids. For A. radioresistens 50v1 (isolated from a spacecraft surface), cultivations yield (1) differential changes in intracellular amino acids, compatible solutes, nucleotide-related metabolites, dicarboxylic acids, and saturated fatty acids and (2) substantial yet differential impacts to extracellular sugar acids, monosaccharides, and organic acids. These combined results suggest that (1) K30 manifests strain-dependent impacts on the intracellular metabolomes and (2) K30 influences extracellular trace element acquisition in both strains. Hence, this work lends support towards the hypothesis that repeated cleansing during spacecraft assembly serve as selective pressures that promote tolerances towards the cleaning conditions.
Institute
California State Polytechnic University, Pomona
DepartmentChemistry & Biochemistry
Last Namemogul
First Namerakesh
Address3801 w. temple ave.
Emailrmogul@cpp.edu
Phone9098694309
Submit Date2022-11-04
Analysis Type DetailGC-MS
Release Date2022-12-06
Release Version1
rakesh mogul rakesh mogul
https://dx.doi.org/10.21228/M8XH73
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001530
Project DOI:doi: 10.21228/M8XH73
Project Title:Metabolomic Profiling of the Spacecraft-Associated Microorganisms.
Project Type:biochemistry
Project Summary:Maintaining low biological contamination in cleanroom-type facilities are critical components to spacecraft assembly (Rummel, 1992; NASA, 2011; Frick et al., 2014) and crewed spacecraft exploration (Spry et al., 2020). In this project we are profiling the metabolomes of cleanroom-associated microorganisms.
Institute:California State Polytechnic University, Pomona
Department:Chemistry & Biochemistry
Laboratory:mogul lab
Last Name:mogul
First Name:rakesh
Address:3801 w. temple ave.
Email:rmogul@cpp.edu
Phone:9098694309
Funding Source:NASA

Subject:

Subject ID:SU002469
Subject Type:Bacteria
Subject Species:Acinetobacter
Taxonomy ID:Acinetobacter
Gender:Not applicable

Factors:

Subject type: Bacteria; Subject species: Acinetobacter (Factor headings shown in green)

mb_sample_id local_sample_id strain type treatment
SA23744623A. johnsonii 2P08AA broth 0.1% K30
SA23744724A. johnsonii 2P08AA broth 0.1% K30
SA23744822A. johnsonii 2P08AA broth 0.1% K30
SA23744319A. johnsonii 2P08AA broth 0% K30
SA23744420A. johnsonii 2P08AA broth 0% K31
SA23744521A. johnsonii 2P08AA broth 0% K32
SA23745212A. johnsonii 2P08AA cell 0.1% K30
SA23745311A. johnsonii 2P08AA cell 0.1% K30
SA23745410A. johnsonii 2P08AA cell 0.1% K30
SA2374497A. johnsonii 2P08AA cell 0% K30
SA2374508A. johnsonii 2P08AA cell 0% K31
SA2374519A. johnsonii 2P08AA cell 0% K32
SA23745816A. radioresistens 50v1 broth 0.1% K30
SA23745918A. radioresistens 50v1 broth 0.1% K30
SA23746017A. radioresistens 50v1 broth 0.1% K30
SA23745515A. radioresistens 50v1 broth 0% K30
SA23745613A. radioresistens 50v1 broth 0% K30
SA23745714A. radioresistens 50v1 broth 0% K30
SA2374646A. radioresistens 50v1 cell 0.1% K30
SA2374655A. radioresistens 50v1 cell 0.1% K30
SA2374664A. radioresistens 50v1 cell 0.1% K30
SA2374612A. radioresistens 50v1 cell 0% K30
SA2374623A. radioresistens 50v1 cell 0% K30
SA2374631A. radioresistens 50v1 cell 0% K30
Showing results 1 to 24 of 24

Collection:

Collection ID:CO002462
Collection Summary:see attached
Collection Protocol Filename:cultivation_methods.pdf
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR002481
Treatment Summary:0% vs. 0.1% v/v kleenol 30, 0.2x M9, 25 µM Fe, 28˚C

Sample Preparation:

Sampleprep ID:SP002475
Sampleprep Summary:see attached
Sampleprep Protocol Filename:GCTOFMS_methods.pdf

Combined analysis:

Analysis ID AN003879
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode POSITIVE
Units counts

Chromatography:

Chromatography ID:CH002874
Instrument Name:Agilent 6890N
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS003620
Analysis ID:AN003879
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:Electron ionization was conducted as 70 eV with an ion source temperature of 250°C. Spectra were recorded using an acquisition rate of 17 scans/second across a mass range of 85-500 Da.
Ion Mode:POSITIVE
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