Summary of Study ST002384

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001534. The data can be accessed directly via it's Project DOI: 10.21228/M8DH62 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002384
Study TitleLipidomics study of curcumin and epigallocatechin gallate on IgE-mediated degranulation of RBL-2H3 cells
Study SummaryThis study provides novel insights into the potential anti-allergic mechanism of food-derived curcumin and EGCG, and then facilitates the discovery of drug target and the development of diagnostic methods for allergic diseases.
Institute
College of Marine Food and Biological Engineering, Jimei University
Last NameYang
First NameZhiqiang
AddressNo. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email201911832016@jmu.edu.cn
Phone0592-6181487
Submit Date2022-07-19
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2022-12-27
Release Version1
Zhiqiang Yang Zhiqiang Yang
https://dx.doi.org/10.21228/M8DH62
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001534
Project DOI:doi: 10.21228/M8DH62
Project Title:Lipidomics Investigation of Curcumin and Epigallocatechin Gallate on IgE-Mediated Degranulation of RBL-2H3 Cells
Project Summary:In this study, an IgE-mediated RBL-2H3 cell degranulation model was firstly developed. Then, ultra-high-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) was used to perform the non-targeted lipidomics study, in order to investigate the effects and mechanism of curcumin/EGCG on IgE-mediated RBL-2H3 cells degranulation.
Institute:College of Marine Food and Biological Engineering, Jimei University
Last Name:Yang
First Name:Zhiqiang
Address:No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China
Email:201911832016@jmu.edu.cn
Phone:0592-6181487

Subject:

Subject ID:SU002473
Subject Type:Cultured cells
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Cultured cells; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA237898C10-1CAfter allergy, 10uM curcumin interventionfor 1h
SA237899C10-1BAfter allergy, 10uM curcumin interventionfor 1h
SA237900C10-1DAfter allergy, 10uM curcumin interventionfor 1h
SA237901C10-1B-NAfter allergy, 10uM curcumin interventionfor 1h
SA237902C10-1A-NAfter allergy, 10uM curcumin interventionfor 1h
SA237903C10-1AAfter allergy, 10uM curcumin interventionfor 1h
SA237904C10-1D-NAfter allergy, 10uM curcumin interventionfor 1h
SA237905C10-1C-NAfter allergy, 10uM curcumin interventionfor 1h
SA237906C10-3C-NAfter allergy, 10uM curcumin interventionfor 3h
SA237907C10-3B-NAfter allergy, 10uM curcumin interventionfor 3h
SA237908C10-3A-NAfter allergy, 10uM curcumin interventionfor 3h
SA237909C10-3AAfter allergy, 10uM curcumin interventionfor 3h
SA237910C10-3D-NAfter allergy, 10uM curcumin interventionfor 3h
SA237911C10-3BAfter allergy, 10uM curcumin interventionfor 3h
SA237912C10-3CAfter allergy, 10uM curcumin interventionfor 3h
SA237913C10-3DAfter allergy, 10uM curcumin interventionfor 3h
SA237914E200-1B-NAfter allergy, 200uM EGCG interventionfor 1h
SA237915E200-1DAfter allergy, 200uM EGCG interventionfor 1h
SA237916E200-1A-NAfter allergy, 200uM EGCG interventionfor 1h
SA237917E200-1CAfter allergy, 200uM EGCG interventionfor 1h
SA237918E200-1D-NAfter allergy, 200uM EGCG interventionfor 1h
SA237919E200-1C-NAfter allergy, 200uM EGCG interventionfor 1h
SA237920E200-1BAfter allergy, 200uM EGCG interventionfor 1h
SA237921E200-1AAfter allergy, 200uM EGCG interventionfor 1h
SA237922E200-3CAfter allergy, 200uM EGCG interventionfor 3h
SA237923E200-3A-NAfter allergy, 200uM EGCG interventionfor 3h
SA237924E200-3B-NAfter allergy, 200uM EGCG interventionfor 3h
SA237925E200-3DAfter allergy, 200uM EGCG interventionfor 3h
SA237926E200-3BAfter allergy, 200uM EGCG interventionfor 3h
SA237927E200-3AAfter allergy, 200uM EGCG interventionfor 3h
SA237928E200-3C-NAfter allergy, 200uM EGCG interventionfor 3h
SA237929E200-3D-NAfter allergy, 200uM EGCG interventionfor 3h
SA237930C30-1A-NAfter allergy, 30uM curcumin interventionfor 1h
SA237931C30-1BAfter allergy, 30uM curcumin interventionfor 1h
SA237932C30-1DAfter allergy, 30uM curcumin interventionfor 1h
SA237933C30-1CAfter allergy, 30uM curcumin interventionfor 1h
SA237934C30-1B-NAfter allergy, 30uM curcumin interventionfor 1h
SA237935C30-1C-NAfter allergy, 30uM curcumin interventionfor 1h
SA237936C30-1D-NAfter allergy, 30uM curcumin interventionfor 1h
SA237937C30-1AAfter allergy, 30uM curcumin interventionfor 1h
SA237938C30-3B-NAfter allergy, 30uM curcumin interventionfor 3h
SA237939C30-3A-NAfter allergy, 30uM curcumin interventionfor 3h
SA237940C30-3C-NAfter allergy, 30uM curcumin interventionfor 3h
SA237941C30-3D-NAfter allergy, 30uM curcumin interventionfor 3h
SA237942C30-3BAfter allergy, 30uM curcumin interventionfor 3h
SA237943C30-3CAfter allergy, 30uM curcumin interventionfor 3h
SA237944C30-3DAfter allergy, 30uM curcumin interventionfor 3h
SA237945C30-3AAfter allergy, 30uM curcumin interventionfor 3h
SA237946E500-1D-NAfter allergy, 500uM EGCG interventionfor 1h
SA237947E500-1B-NAfter allergy, 500uM EGCG interventionfor 1h
SA237948E500-1A-NAfter allergy, 500uM EGCG interventionfor 1h
SA237949E500-1AAfter allergy, 500uM EGCG interventionfor 1h
SA237950E500-1DAfter allergy, 500uM EGCG interventionfor 1h
SA237951E500-1C-NAfter allergy, 500uM EGCG interventionfor 1h
SA237952E500-1BAfter allergy, 500uM EGCG interventionfor 1h
SA237953E500-1CAfter allergy, 500uM EGCG interventionfor 1h
SA237954E500-3CAfter allergy, 500uM EGCG interventionfor 3h
SA237955E500-3DAfter allergy, 500uM EGCG interventionfor 3h
SA237956E500-3D-NAfter allergy, 500uM EGCG interventionfor 3h
SA237957E500-3C-NAfter allergy, 500uM EGCG interventionfor 3h
SA237958E500-3AAfter allergy, 500uM EGCG interventionfor 3h
SA237959E500-3BAfter allergy, 500uM EGCG interventionfor 3h
SA237960E500-3A-NAfter allergy, 500uM EGCG interventionfor 3h
SA237961E500-3B-NAfter allergy, 500uM EGCG interventionfor 3h
SA2379620-1AAllergy group
SA2379630-3A-NAllergy group
SA2379640-3D-NAllergy group
SA2379650-3C-NAllergy group
SA2379660-3B-NAllergy group
SA2379670-1D-NAllergy group
SA2379680-1A-NAllergy group
SA2379690-3AAllergy group
SA2379700-3BAllergy group
SA2379710-3CAllergy group
SA2379720-1B-NAllergy group
SA2379730-1C-NAllergy group
SA2379740-1CAllergy group
SA2379750-1DAllergy group
SA2379760-3DAllergy group
SA2379770-1BAllergy group
SA237978PBS-1A-NegatveControl
SA237979PBS-3BControl
SA237980PBS-3CControl
SA237981PBS-3AControl
SA237982PBS-1DControl
SA237983PBS-1BControl
SA237984PBS-1CControl
SA237985PBS-3DControl
SA237986PBS-1AControl
SA237987PBS-3B-NControl
SA237988PBS-3C-NControl
SA237989PBS-3A-NControl
SA237990PBS-1D-NControl
SA237991PBS-1B-NControl
SA237992PBS-1C-NControl
SA237993PBS-3D-NControl
Showing results 1 to 96 of 96

Collection:

Collection ID:CO002466
Collection Summary:RBL-2H3 cells were incubated with 200 ng/mL of IgE for 18 h, followed by co-stimulation with 500 ng/mL DNP-BSA for the Alg group, polyphenolic substances (curcumin or EGCG) and 500 ng/mL DNP-BSA for the intervention group, and the same volume of PBS solution for the PBS group. The cell samples were collected at two different time points, 1 h and 3 h after the effect, respectively.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR002485
Treatment Summary:RBL-2H3 cells were incubated with 200 ng/mL of IgE for 18 h, followed by co-stimulation with 500 ng/mL DNP-BSA for the Alg group, polyphenolic substances (curcumin or EGCG) and 500 ng/mL DNP-BSA for the intervention group, and the same volume of PBS solution for the PBS group.

Sample Preparation:

Sampleprep ID:SP002479
Sampleprep Summary:The MeOH/MTBE/H2O system was used for the extraction of lipid metabolites from cell samples. Cell culture dishes stored at -80 °C were first removed on ice and 1 mL of methanolic solution containing internal standards was added, consisting of 1 μg/mL FFA (C16:0)-d3 and FFA (C18:0)-d3, 0.87 μg/mL LPC (19:0) and SM (d18:1/12:0), 1.12 μg/mL PC (38: 0), 0.92 μg/mL PE (30:0), 0.67 μg/mL TG (45:0), and 0.47 μg/mL Cer (d18:1/17:0). The cells were transferred to 5 mL Eppendorf centrifuge tubes by scraping with a cell scraper, adding 2.5 mL of MTBE and vortexing for 30 s. The cells were further shaken for 30 min at 10 °C and 1000 rpm using a thermostatic mixer. 750 μL of ultrapure water was added, vortexed for 60 s and allowed to stand for 5 min, and then centrifuged at 12,000 g for 10 min at 6 °C. After separation of the two phases, 400 μL of the hydrophobic phase was collected and dried in a cryogenic vacuum centrifuge concentrator, and the resulting lyophilized sample was stored at -80 °C.

Combined analysis:

Analysis ID AN003883 AN003884
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters Acquity BEH C8 (100 x 2.1mm,1.7um) Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH002877
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH002878
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C8 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003623
Analysis ID:AN003883
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibular
Ion Mode:POSITIVE
  
MS ID:MS003624
Analysis ID:AN003884
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibular
Ion Mode:NEGATIVE
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