Summary of Study ST002419

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001557. The data can be accessed directly via it's Project DOI: 10.21228/M8FD7Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002419
Study TitleMetabolomic changes in growth of E. colik at four timespoints in MOPS rich and minimum medium
Study TypeMS quantitative analysis
Study SummaryMetabolomic changes were profiled using untargeted metabolomics in E. coli following its growth in two medium types. E. coli strain was grown in MOPS rich and minimum medium and samples were taken at four timepoints: the beginning of lag phase, end of lag phase, mid-log phase, and beginning of the stationary phase.
Institute
Duke Kunshan University
Last NameCao
First NameHuansheng
Address8 Duke Ave, Kunshan, 215316, China
Emailhc284@duke.edu
Phone04803885366
Submit Date2022-10-24
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2023-09-06
Release Version1
Huansheng Cao Huansheng Cao
https://dx.doi.org/10.21228/M8FD7Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001557
Project DOI:doi: 10.21228/M8FD7Q
Project Title:Metabolomic changes in growth of E. coli at four timepoints in MOPS rich and minimum medium
Project Type:MS quantitative analysis
Project Summary:Metabolomic changes were profiled using untargeted metabolomics in E coli following its growth in two medium types: MOPS rich and minimum medium. the samples were taken at the beginning of lag phase, end of lag phase, middle log phase, beginning of stationary phase. the goal was to follow the metabolic changes during a complete growth cycle.
Institute:Duke Kunshan University
Department:Division of Natural and Applied Sciences
Last Name:Cao
First Name:Huansheng
Address:8 Duke Ave, Kunshan, 215316, China; City: Kunshan
Email:hc284@duke.edu
Phone:36657030
Funding Source:NSF of China (NSFC)

Subject:

Subject ID:SU002508
Subject Type:Bacteria
Subject Species:Escherichia coli
Taxonomy ID:562

Factors:

Subject type: Bacteria; Subject species: Escherichia coli (Factor headings shown in green)

mb_sample_id local_sample_id treatment
SA242269383235MOPS minimal medium - E. coli cells
SA242270383234MOPS minimal medium - E. coli cells
SA242271383241MOPS minimal medium - E. coli cells
SA242272383243MOPS minimal medium - E. coli cells
SA242273383237MOPS minimal medium - E. coli cells
SA242274383240MOPS minimal medium - E. coli cells
SA242275383247MOPS minimal medium - E. coli cells
SA242276383246MOPS minimal medium - E. coli cells
SA242277383242MOPS minimal medium - E. coli cells
SA242278383238MOPS minimal medium - E. coli cells
SA242279383236MOPS minimal medium - E. coli cells
SA242280383239MOPS minimal medium - E. coli cells
SA242257383224MOPS Rich medium - E. coli cells
SA242258383230MOPS Rich medium - E. coli cells
SA242259383229MOPS Rich medium - E. coli cells
SA242260383226MOPS Rich medium - E. coli cells
SA242261383232MOPS Rich medium - E. coli cells
SA242262383228MOPS Rich medium - E. coli cells
SA242263383231MOPS Rich medium - E. coli cells
SA242264383225MOPS Rich medium - E. coli cells
SA242265383223MOPS Rich medium - E. coli cells
SA242266383245MOPS Rich medium - E. coli cells
SA242267383244MOPS Rich medium - E. coli cells
SA242268383227MOPS Rich medium - E. coli cells
Showing results 1 to 24 of 24

Collection:

Collection ID:CO002501
Collection Summary:metabolomic changes were profiled using untargeted metabolomics in E coli following its growth in two medium types
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR002520
Treatment Summary:two growth conditions are set up: minimal and rich MOPS medium

Sample Preparation:

Sampleprep ID:SP002514
Sampleprep Summary:metabolomic changes were profiled using untargeted metabolomics in E coli following its growth in two medium types

Combined analysis:

Analysis ID AN003940
Analysis type MS
Chromatography type Normal phase
Chromatography system Leco Pegasus IV GC
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode POSITIVE
Units mmol/L

Chromatography:

Chromatography ID:CH002917
Chromatography Summary:GCTOF MS
Methods Filename:Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf
Instrument Name:Leco Pegasus IV GC
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Chromatography Type:Normal phase

MS:

MS ID:MS003676
Analysis ID:AN003940
Instrument Name:Leco Pegasus IV TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:see protocol file
Ion Mode:POSITIVE
Analysis Protocol File:Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf
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