Summary of Study ST002437
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001569. The data can be accessed directly via it's Project DOI: 10.21228/M8WD9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002437 |
| Study Title | Metabolomic analysis in wildtype and Isg15 knockout mice after sham or transverse aortic constriction surgery |
| Study Summary | Left ventricular tissues were isolated from wildtype and Isg15 knockout mice 8 weeks after sham or transverse aortic constriction and the metabolomic differences were compared. |
| Institute | St. Michael's Hospital |
| Last Name | Advani |
| First Name | Andrew |
| Address | 6-151, 61 Queen Street East, Toronto, Ontario. M5C 2t2 |
| andrew.advani@unityhealth.to | |
| Phone | 4163604000 |
| Submit Date | 2023-01-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001569 |
| Project DOI: | doi: 10.21228/M8WD9H |
| Project Title: | Metabolomic analysis in wildtype and Isg15 knockout mice after sham or transverse aortic constriction surgery |
| Project Type: | Untargeted metabolomic analysis |
| Project Summary: | Left ventricular tissue was isolated from wildtype and Isg15 knockout mice 8 weeks after sham or transverse aortic constriction surgery and the metabolomic differences across the samples were compared. |
| Institute: | St. Michael's Hospital |
| Department: | Keenan Research Centre for Biomedical Science |
| Laboratory: | Advani Lab |
| Last Name: | Advani |
| First Name: | Andrew |
| Address: | 6-151, 61 Queen St. East, Toronto, Ontario. M5C 2T2 |
| Email: | andrew.advani@unityhealth.to |
| Phone: | 4163604000 |
Subject:
| Subject ID: | SU002526 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Surgical condition |
|---|---|---|---|
| SA243600 | Sample 10 | ISG15 KO | Sham |
| SA243601 | Sample 7 | ISG15 KO | Sham |
| SA243602 | Sample 8 | ISG15 KO | Sham |
| SA243603 | Sample 6 | ISG15 KO | Sham |
| SA243604 | Sample 18 | ISG15 KO | TAC |
| SA243605 | Sample 16 | ISG15 KO | TAC |
| SA243606 | Sample 17 | ISG15 KO | TAC |
| SA243607 | Sample 19 | ISG15 KO | TAC |
| SA243608 | Sample 2 | Wild type | Sham |
| SA243609 | Sample 4 | Wild type | Sham |
| SA243610 | Sample 3 | Wild type | Sham |
| SA243611 | Sample 11 | Wild type | TAC |
| SA243612 | Sample 12 | Wild type | TAC |
| SA243613 | Sample 13 | Wild type | TAC |
| SA243614 | Sample 14 | Wild type | TAC |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO002519 |
| Collection Summary: | Mouse tissue frozen samples were reconstituted in 500 uL 80% MeCN containing 1 uM caffeine-d9 internal standard. The samples were homogenized, vortexed and centrifuged 21000 x g for two minutes at room temperature (23°C). |
| Sample Type: | Cardiac tissue |
Treatment:
| Treatment ID: | TR002538 |
| Treatment Summary: | Wildtype and ISG15 knockout mice underwent sham or transverse aortic constriction surgery. |
Sample Preparation:
| Sampleprep ID: | SP002532 |
| Sampleprep Summary: | 200 uL of each sample supernatants were diluted with 200 uL H2O and 100 uL used for the LC-MS analysis. LC-MS analyses were performed using Vanquish UPLC with 0-100% 10 minutes gradient (0.1% FA - 0.1%FA/MeCN) at 200 uL/min flow rate on Phenomenex Luna Omega 3 um Polar C18 100A 50x2.1 mm (RP-LC-MS). 10 uL injection volumes were used for each run. Mass spectra were acquired using Thermo Orbitrap QE+ spectrometer in positive and negative modes in separate runs. |
Chromatography:
| Chromatography ID: | CH002936 |
| Chromatography Summary: | LC-MS analyses were performed using Vanquish UPLC with 0-100% 10 minutes gradient (0.1% FA - 0.1%FA/MeCN) at 200 uL/min flow rate on Phenomenex Luna Omega 3 um Polar C18 100A 50x2.1 mm (RP-LC-MS). 10 uL injection volumes were used for each run. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Luna Omega Polar C18(50 x 2.1mm, 3um. 100A) |
| Column Temperature: | 23 |
| Flow Gradient: | 0-100% 10 minutes gradient |
| Flow Rate: | 200 uL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN003970 |
| Analysis Type: | MS |
| Chromatography ID: | CH002936 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST002437_AN003970_Results.txt |
| Units: | Peak Area |
| Analysis ID: | AN003971 |
| Analysis Type: | MS |
| Chromatography ID: | CH002936 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Results File: | ST002437_AN003971_Results.txt |
| Units: | Peak Area |
