Summary of Study ST002439

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001571. The data can be accessed directly via it's Project DOI: 10.21228/M8MX49 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002439
Study TitleCharacterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (PETALS Cohort)
Study TypeProspective Cohort Study
Study SummaryPETALS is a prospective cohort of multi-racial/ethnic pregnant women recruited in early pregnancy at Kaiser Permanente Northern California which aimed to examine environmental factors in association with pregnancy, perinatal, and childhood outcomes. Please contact the cohort PI Assiamira Ferrara (Assiamira.Ferrara@kp.org) and Co-I Yeyi Zhu (yeyi.zhu@kp.org) for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. PETALS is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Kaiser Permanente
DepartmentDivision of Research
Last NameFerrara
First NameAssiamira
Address2000 Broadway, Oakland, CA 94612
Emailassiamira.ferrara@kp.org
Phone(510) 891-3588
Submit Date2023-01-12
Total Subjects292
Study CommentsHHEAR Project EM19-0009, ECHO Project EC0374
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2024-01-12
Release Version1
Assiamira Ferrara Assiamira Ferrara
https://dx.doi.org/10.21228/M8MX49
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001571
Project DOI:doi: 10.21228/M8MX49
Project Title:Characterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (PETALS Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project Viva, Atlanta ECHO, ARCH Cohort, and PETALS. This project will generate new untargeted metabolomics profiling data at two time-points in pregnancy for all women in order to identify trimester-specific metabolomics signatures and longitudinal metabolomics trajectories associated with offspring birth size; identify the contribution of maternal sociodemographic and perinatal characteristics to the metabolomics signatures.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:(984) 377-0693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The PETALS ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002528
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA243700PZFe_HHEAR_22HHEAR Pool
SA243701PZFe_HHEAR_23HHEAR Pool
SA243702PZFe_HHEAR_21HHEAR Pool
SA243703PZFe_HHEAR_19HHEAR Pool
SA243704PZFe_HHEAR_18HHEAR Pool
SA243705PZFe_HHEAR_24HHEAR Pool
SA243706PZFe_HHEAR_20HHEAR Pool
SA243707PZFe_HHEAR_25HHEAR Pool
SA243708PZFe_HHEAR_30HHEAR Pool
SA243709PZFe_HHEAR_1HHEAR Pool
SA243710PZFe_HHEAR_28HHEAR Pool
SA243711PZFe_HHEAR_27HHEAR Pool
SA243712PZFe_HHEAR_26HHEAR Pool
SA243713PZFe_HHEAR_17HHEAR Pool
SA243714PZFe_HHEAR_29HHEAR Pool
SA243715PZFe_HHEAR_6HHEAR Pool
SA243716PZFe_HHEAR_16HHEAR Pool
SA243717PZFe_HHEAR_5HHEAR Pool
SA243718PZFe_HHEAR_2HHEAR Pool
SA243719PZFe_HHEAR_3HHEAR Pool
SA243720PZFe_HHEAR_4HHEAR Pool
SA243721PZFe_HHEAR_8HHEAR Pool
SA243722PZFe_HHEAR_7HHEAR Pool
SA243723PZFe_HHEAR_9HHEAR Pool
SA243724PZFe_HHEAR_15HHEAR Pool
SA243725PZFe_HHEAR_13HHEAR Pool
SA243726PZFe_HHEAR_14HHEAR Pool
SA243727PZFe_HHEAR_12HHEAR Pool
SA243728PZFe_HHEAR_10HHEAR Pool
SA243729PZFe_HHEAR_11HHEAR Pool
SA243730PZFe_NIST_8NIST Pool
SA243731PZFe_NIST_9NIST Pool
SA243732PZFe_NIST_12NIST Pool
SA243733PZFe_NIST_11NIST Pool
SA243734PZFe_NIST_10NIST Pool
SA243735PZFe_NIST_7NIST Pool
SA243736PZFe_NIST_6NIST Pool
SA243737PZFe_NIST_1NIST Pool
SA243738PZFe_NIST_3NIST Pool
SA243739PZFe_NIST_2NIST Pool
SA243740PZFe_NIST_4NIST Pool
SA243741PZFe_NIST_5NIST Pool
SA243742PZFe_SP_11Study Pool
SA243743PZFe_SP_9Study Pool
SA243744PZFe_SP_10Study Pool
SA243745PZFe_SP_14Study Pool
SA243746PZFe_SP_8Study Pool
SA243747PZFe_SP_13Study Pool
SA243748PZFe_SP_12Study Pool
SA243749PZFe_SP_3Study Pool
SA243750PZFe_SP_1Study Pool
SA243751PZFe_SP_15Study Pool
SA243752PZFe_SP_2Study Pool
SA243753PZFe_SP_4Study Pool
SA243754PZFe_SP_6Study Pool
SA243755PZFe_SP_7Study Pool
SA243756PZFe_SP_5Study Pool
SA243757PZFe_SP_26Study Pool
SA243758PZFe_SP_25Study Pool
SA243759PZFe_SP_27Study Pool
SA243760PZFe_SP_28Study Pool
SA243761PZFe_SP_30Study Pool
SA243762PZFe_SP_16Study Pool
SA243763PZFe_SP_24Study Pool
SA243764PZFe_SP_29Study Pool
SA243765PZFe_SP_19Study Pool
SA243766PZFe_SP_23Study Pool
SA243767PZFe_SP_17Study Pool
SA243768PZFe_SP_20Study Pool
SA243769PZFe_SP_21Study Pool
SA243770PZFe_SP_18Study Pool
SA243771PZFe_SP_22Study Pool
SA243772PZFe_S_204Study Sample
SA243773PZFe_S_205Study Sample
SA243774PZFe_S_208Study Sample
SA243775PZFe_S_209Study Sample
SA243776PZFe_S_203Study Sample
SA243777PZFe_S_207Study Sample
SA243778PZFe_S_206Study Sample
SA243779PZFe_S_200Study Sample
SA243780PZFe_S_210Study Sample
SA243781PZFe_S_196Study Sample
SA243782PZFe_S_197Study Sample
SA243783PZFe_S_198Study Sample
SA243784PZFe_S_201Study Sample
SA243785PZFe_S_199Study Sample
SA243786PZFe_S_202Study Sample
SA243787PZFe_S_217Study Sample
SA243788PZFe_S_222Study Sample
SA243789PZFe_S_221Study Sample
SA243790PZFe_S_220Study Sample
SA243791PZFe_S_223Study Sample
SA243792PZFe_S_224Study Sample
SA243793PZFe_S_226Study Sample
SA243794PZFe_S_225Study Sample
SA243795PZFe_S_219Study Sample
SA243796PZFe_S_218Study Sample
SA243797PZFe_S_213Study Sample
SA243798PZFe_S_212Study Sample
SA243799PZFe_S_214Study Sample
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Collection:

Collection ID:CO002521
Collection Summary:NA
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002540
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP002534
Sampleprep Summary:Serum samples (600 µL per tube) were shipped from Kaiser Permanente Mosswood Research Lab to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the study samples to a new set of tubes and used them for the analysis. An additional 10 µL was taken from the original study sample and transferred to another tube to make the total study pool for this project, and then distributed with 50 µL per aliquot, used as Quality control study pools (QC study pools) throughout the whole analysis. All sample aliquots (50 µL each) and QC study pools (50 µL each) were stored at -80° C until the day for sample preparation. HHEAR reference plasma (50 µL each) and NIST serum (909c) reference material (50 µL each) were provided by the NC HHEAR Hub. The LC-MS grade water (50 µL) was used as blank. Sample preparation was conducted by batch, and all samples (except blank) were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference serum, and blanks, were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:4℃
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN003976
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units m/z

Chromatography:

Chromatography ID:CH002939
Chromatography Summary:Reverse phase
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50 oC
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8oC
Solvent A:Water with 0.1% Formic Acid
Solvent B:Methanol with 0.1% Formic Acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003710
Analysis ID:AN003976
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragment Voltage:ES+
Fragmentation Method:CID
Ion Spray Voltage:3.5kV
Ionization:ES+
Mass Accuracy:5 ppm
Dataformat:Profile
Desolvation Gas Flow:55
Desolvation Temperature:400 oC
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