Summary of Study ST002475

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001598. The data can be accessed directly via it's Project DOI: 10.21228/M84Q58 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002475
Study TitlePsychobiotic Lactobacillus plantarum JYLP-326 relieves anxiety, depression, and insomnia symptoms in test anxious college students via modulating the gut microbiota and its metabolism
Study SummaryTest anxiety frequently occurs in college students and harms their physical and psychological health, but suitable interventions or therapeutical strategies are still missing. The present study aims to evaluate the potential effects of Lactobacillus plantarum JYLP-326 on test anxious college students. Sixty anxious students were enrolled and randomly allocated to the placebo group and the probiotic group, which were instructed to take placebo and JYLP-326 products twice per day for three weeks, respectively. Thirty unanxious students with no treatments were assigned to a regular control group. The anxiety, depression, and insomnia questionnaires were used to measure students’ mental states at the baseline and the end of this study. 16S rRNA sequencing and untargeted metabolomics were performed to analyze the changes in the gut microbiota and fecal metabolism. The questionnaire results suggested that JYLP-326 administration could relieve the symptoms of anxiety, depression, and insomnia in test anxious students. The gut microbiomes of the placebo group showed a significantly greater α diversity index than the control group (p < 0.05). An increased abundance of Bacteroides and Roseburia was observed in the placebo group, and the relative abundance of Prevotella and Bifidobacterium decreased. Whereas JYLP-326 administration could partly restore the disturbed gut microbiota. Additionally, test anxiety was correlation with disordered fecal metabolomics such as a higher Ethyl sulfate and a lower Cyclohexylamine, which could be reversed after taking JYLP-326. Furthermore, the changed microbiota and fecal metabolites were significantly associated with anxiety-related symptoms. These results indicated that the intervention of L. plantarum JYLP-326 could be an effective strategy to alleviate anxiety, depression and insomnia in test anxious college students.
Institute
Nanchang university
Last NameRuizhe
First NameZhu
AddressNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, China
Emailarthurzhu233@outlook.com
Phone18779116254
Submit Date2023-02-10
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2023-02-16
Release Version1
Zhu Ruizhe Zhu Ruizhe
https://dx.doi.org/10.21228/M84Q58
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001598
Project DOI:doi: 10.21228/M84Q58
Project Title:Psychobiotic Lactobacillus plantarum JYLP-326 relieves anxiety, depression, and insomnia symptoms in test anxious college students via modulating the gut microbiota and its metabolism
Project Summary:Test anxiety frequently occurs in college students and harms their physical and psychological health, but suitable interventions or therapeutical strategies are still missing. The present study aims to evaluate the potential effects of Lactobacillus plantarum JYLP-326 on test anxious college students. Sixty anxious students were enrolled and randomly allocated to the placebo group and the probiotic group, which were instructed to take placebo and JYLP-326 products twice per day for three weeks, respectively. Thirty unanxious students with no treatments were assigned to a regular control group. The anxiety, depression, and insomnia questionnaires were used to measure students’ mental states at the baseline and the end of this study. 16S rRNA sequencing and untargeted metabolomics were performed to analyze the changes in the gut microbiota and fecal metabolism. The questionnaire results suggested that JYLP-326 administration could relieve the symptoms of anxiety, depression, and insomnia in test anxious students. The gut microbiomes of the placebo group showed a significantly greater α diversity index than the control group (p < 0.05). An increased abundance of Bacteroides and Roseburia was observed in the placebo group, and the relative abundance of Prevotella and Bifidobacterium decreased. Whereas JYLP-326 administration could partly restore the disturbed gut microbiota. Additionally, test anxiety was correlation with disordered fecal metabolomics such as a higher Ethyl sulfate and a lower Cyclohexylamine, which could be reversed after taking JYLP-326. Furthermore, the changed microbiota and fecal metabolites were significantly associated with anxiety-related symptoms. These results indicated that the intervention of L. plantarum JYLP-326 could be an effective strategy to alleviate anxiety, depression and insomnia in test anxious college students.
Institute:Nanchang university
Last Name:Ruizhe
First Name:Zhu
Address:National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, China
Email:arthurzhu233@outlook.com
Phone:18779116254

Subject:

Subject ID:SU002565
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id treatment
SA247814PHP9Control
SA247815PHP8Control
SA247816PHP10Control
SA247817PHP11Control
SA247818PHP12Control
SA247819PHP7Control
SA247820PHP6Control
SA247821PHP2Control
SA247822PHP3Control
SA247823PHP4Control
SA247824PHP5Control
SA247825PHP13Control
SA247826PHP15Control
SA247827PHP22Control
SA247828PHP21Control
SA247829PHP23Control
SA247830PHP24Control
SA247831PHP25Control
SA247832PHP20Control
SA247833PHP19Control
SA247834NHP1Control
SA247835PHP16Control
SA247836PHP17Control
SA247837PHP18Control
SA247838PHP14Control
SA247839PHP1Control
SA247840NHP18Control
SA247841NHP17Control
SA247842NHP16Control
SA247843NHP15Control
SA247844NHP19Control
SA247845NHP20Control
SA247846NHP24Control
SA247847NHP23Control
SA247848NHP22Control
SA247849NHP21Control
SA247850NHP13Control
SA247851NHP12Control
SA247852NHP5Control
SA247853NHP4Control
SA247854NHP3Control
SA247855NHP2Control
SA247856NHP6Control
SA247857NHP7Control
SA247858NHP11Control
SA247859NHP10Control
SA247860NHP9Control
SA247861NHP8Control
SA247862NHP25Control
SA247863NHP14Control
SA247864PP2Placebo
SA247865NP5Placebo
SA247866PP3Placebo
SA247867PP4Placebo
SA247868PP5Placebo
SA247869NP6Placebo
SA247870NP7Placebo
SA247871NP11Placebo
SA247872NP12Placebo
SA247873NP10Placebo
SA247874NP9Placebo
SA247875NP8Placebo
SA247876PP6Placebo
SA247877PP7Placebo
SA247878PP16Placebo
SA247879PP15Placebo
SA247880PP17Placebo
SA247881PP18Placebo
SA247882NP4Placebo
SA247883PP14Placebo
SA247884PP13Placebo
SA247885PP9Placebo
SA247886PP8Placebo
SA247887PP10Placebo
SA247888PP11Placebo
SA247889PP12Placebo
SA247890NP13Placebo
SA247891PP1Placebo
SA247892NP2Placebo
SA247893NP1Placebo
SA247894NP14Placebo
SA247895NP17Placebo
SA247896NP18Placebo
SA247897NP15Placebo
SA247898NP3Placebo
SA247899NP16Placebo
SA247900PPB9Treatment
SA247901PPB10Treatment
SA247902PPB8Treatment
SA247903PPB5Treatment
SA247904PPB3Treatment
SA247905PPB4Treatment
SA247906PPB11Treatment
SA247907PPB6Treatment
SA247908PPB7Treatment
SA247909PPB13Treatment
SA247910PPB18Treatment
SA247911PPB19Treatment
SA247912NPB10Treatment
SA247913PPB17Treatment
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Collection:

Collection ID:CO002558
Collection Summary:The first pooped fecal sample in a day were collected during 3 days after the indicated time points and stored at -80 oC immediately for further research
Sample Type:Feces

Treatment:

Treatment ID:TR002577
Treatment Summary:Out of those 60 students with anxiety and depression facing UNGEE, 30 were selected randomly as the PB group to receive the probiotic product; another 30 received placebo product were regarded as the P group. The 30 un-anxious students were assigned as the healthy control group (HP group).

Sample Preparation:

Sampleprep ID:SP002571
Sampleprep Summary:Stool weighing 100 mg was added with 400 ml extract of methanol-acetonitrile-water (2:2:1, vol/vol/vol), vortexed and centrifuged at 14,000 g for 20min, and the supernatant was collected for further measurement.

Combined analysis:

Analysis ID AN004042 AN004043
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 6600 TripleTOF ABI Sciex 6600 TripleTOF
Ion Mode POSITIVE NEGATIVE
Units Original peak intensity values Original peak intensity values

Chromatography:

Chromatography ID:CH002990
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um)
Column Temperature:25
Flow Gradient:The gradient elution program was as follows: 0 --- 0.5 min, 95% B; 0.5 --- 7 min, B varied linearly from 95% to 65 %; 7 --- 8 min, B varied linearly from 65% to 40%; 8---9 min, B was maintained at 40%; 9---9.1 min, B varied linearly from 40% to 95%; 9.1--12 min, B maintained at 95%.
Flow Rate:0.5 mL/min
Solvent A:100% water; 25 mM ammonium acetate; 25 mM ammonia
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS003789
Analysis ID:AN004042
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:Ion Source Gas1（Gas1）：60，Ion Source Gas2（Gas2）：60，Curtain gas （CUR）：30，source temperature：600℃，IonSapary Voltage Floating （ISVF） The raw data in Wiff format was converted to .mzXML format by ProteoWizard and then converted to .mzXML format using XCMS software.
Ion Mode:POSITIVE
  
MS ID:MS003790
Analysis ID:AN004043
Instrument Name:ABI Sciex 6600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:Ion Source Gas1（Gas1）：60，Ion Source Gas2（Gas2）：60，Curtain gas （CUR）：30，source temperature：600℃，IonSapary Voltage Floating （ISVF） The raw data in Wiff format was converted to .mzXML format by ProteoWizard and then converted to .mzXML format using XCMS software.
Ion Mode:NEGATIVE
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